FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_63.3510000004331c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_63.3510000004331c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97827
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC26112.6689973115806476No Hit
AAAGATTTCTGCAGCTAAAACCAAGTATTACAAAAGCTTAAACATCTTCCT5830.5959499933556176No Hit
GTACAAATCCTTACTGATCTTAACCTTACTATCACTAAGGCGTACATATCT5330.5448393592770912No Hit
AAAGAATCCTTTGTACTAGTCGATGTGATTTTGTTTTCAACTGTTCTTGCT4960.5070174900589817No Hit
ACATTATCAATAGCAGCAGTACTTACAAAACGTAGAAATTCCAAACGAAAC4250.4344403896674742No Hit
GTATTCCCAAGAGAGAATAAAAAACACAACAAGATTGCAAAATCTATTCGA3580.3659521400022489No Hit
GACTATAACATGTGAGAAAAAGAATCGTGGTGTGTTCAAAGATAGAACTGA3540.3618632892759667No Hit
CATCTTATGCTTTATCTTGTTGAATGTTCCTTATTTGCATCATAGATACTG3390.34653009905240884No Hit
ATAGTTTACTGAGAATGTTGATTTCCAATTTCATCCATGTCCCTACAAAGG3050.31177486787901093No Hit
GTTGTATCAGTATTGCTATTTGTACTTGCTTCAATAAGGTCTTCGGTAATA3050.31177486787901093No Hit
GGTTTCAATCATTCATTCTTTCATTCATTCCTTCTTCATTCAAGAAATAGC2800.2862195508397477No Hit
GAAATACTCTTGATGTTGGGATAGAAAGGAAATTCTGAGATACTTGGCAAA2790.2851973381581772No Hit
GTCTTAAAGCAACAACAAAATTAAGACTCCAAATGCATAATCCACTAATCC2690.27497521134247194No Hit
ATACTAAAGCACAAGAACCTGTTAAAGATAGTGAGGCTGTTTTGTAAGCCC2660.27190857329776036No Hit
GTTATTTAGAGAATGACAAGTGACTTAACATTACGTTTCCAGATACCAAAA2560.2616864464820551No Hit
GGTTAACATAAACTTGTAAAGGCTATAGATATCTGGTTGGTGAGTCTGAGT2510.2565753830742024No Hit
ACAATAGAATGGTGTATGATCCCTTCTTTTTCATAGCTCTGTTTCTAATCT2470.2524865323479203No Hit
TCAAAACACTAATTCAGAAGAGAAGACTTTAGAGTCGATGGAAAAATTTGG2360.2412421928506445No Hit
GTTCTATTCTATTCAATGGTGATTCCATTCGAGTACATTAGATGATTCCAT2240.22897564067179818No Hit
CTATTAGTGTTAATTCCATTCCATTCCATTCCATTCCTTTCCATTCCCTTC2220.22693121530865712No Hit
AAACATTGAAGTAGAAAATGAAGCTTTTGATCGGTACATGAGTAATGTGAA2210.22590900262708657No Hit
GTATGTGTATTATCATGGTAAAATGTCCCATCAAATCTGCAATAATCATGA2160.22079793921923394No Hit
GATGTTTGGTTTCTTTATCCACCTAAGATGTTTGGCTTCTTTTTCTTATCT2160.22079793921923394No Hit
AGTGATAGATGGTATTGAATCAGATTATTTCATGTTCTTAGATGCTGGAGA2100.21466466312981078No Hit
CTTCAAAATATCTCAAACGTACTAATGTTTGGTACTTTGGAGGCAACATAC2060.21057581240352868No Hit
CGATTCATATTTGTGATAAACGCAAAATATAGATTCGATTCAAACAAAAAG2020.20648696167724656No Hit
ATCCATAACAATCCAACAACAAGGAATAACTTAACTGAAACAAGTTCAGAC1990.20342032363253498No Hit
TCGTTATCTCTGGTGTTGTTTTAAGTTTTTAGTTGGACACAATCAGTATTC1980.20239811095096447No Hit
ACAAAATACAGTAAACACCTTACATCATCTAAGGAGTTCATGCCACTCATT1910.19524262217997077No Hit
TAATCATCCTGCTGTGCAGTTAATAAACTCATCCAGTATGTATGTATGTGT1880.1921759841352592No Hit
AATGTATCCCTGTCTATTAGTTACCGTAATATGCCAAAAGGTAATGTTCCT1830.18706492072740655No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT1740.17786500659327178No Hit
GGAGGATGGTAATTCCTTTAATTAGGTTTGGGATTACCAATGAATGTTCTC1690.17275394318541917No Hit
ATCTTGAAAGGATTGATTAGTTTCAAAGGGTATTTGGCAGAGGAAACTGTA1640.1676428797775665No Hit
AATAAGAAGTGTGTTTGAAAGATCTCCAAACACTGTTTTATAACCTCTTTG1620.16559845441442547No Hit
ATGAAGAGTAAATCAGTCAAGCCTTTTTTATCTCATGCCTCTTGTAGCTTT1610.16457624173285493No Hit
GATAAATCCAATACAAACGAAGAACATAAATCAGAAAACCAAAATCTTTGC1610.16457624173285493No Hit
ATGAAGAGTAGATGGATTTTAACCAAATAACGAGTGATTGATACAATGAAG1590.1625318163697139No Hit
TATAAAGCGAGATATGAAACTTTTCTCTGGGAGTCTTCAGAATCATGTTCA1590.1625318163697139No Hit
GGTTTGGGATTACCAATGAATGTTCTCTCTCTCGCTTGTTATGCTTCTACT1570.16048739100657283No Hit
AATCTGGAGGTAGGAATCAATTTTAAACCAAAACTTATGCTCAAACGTTTA1540.15742075296186125No Hit
GAGTTAGGATATGATTTACGACATTGAATCTTGGACTGATTAATTCTTTGC1480.15128747687243807No Hit
ATTCCATTCCATTCCATTCCATTCCATTCCATTCCGTTCCATTCCATTCCA1460.14924305150929704No Hit
GAGTACATTTAATGATTGCTTTGTTTTCAATTCGAAGATGATTACATTGGA1460.14924305150929704No Hit
GTTATATGTAACGTTCAAGTAGTTTTCTGGAATATCTGAATAAGAAGCGAA1390.14208756273830334No Hit
ACAACATACCAAAACTTTTGGGATGCAATAAAAGTTGCACTAAGGGAGAAA1380.1410653500567328No Hit
GACTCCAAACATTATCAATAGCAGCAGTACTTACAAAACGTAGAAATTCCA1380.1410653500567328No Hit
CATCAAAAAGAGTTGGAGAGGAAACAGAGACATCTATTTTTCTTAACCTAA1370.14004313737516227No Hit
AGTTACAGGAATCAATTTCAAATCAATGTTTATGCTAACGGTAATCTAACA1360.13902092469359176No Hit
CTATCAATTTTTCTCGTCTTTTTCTTGTTATTCCCAGATGATTTACCTTGG1360.13902092469359176No Hit
AGACAGAGAAAGAGAAAGAGAGATCATTCCTATTGAAAACAAGAATAAGTC1360.13902092469359176No Hit
ATTTAGTGTTATTGTAACATGAAAGCGGTCTTGCAACTAGGAAAGAAACAT1350.1379987120120212No Hit
GATCTGTGTGGTTAAGTGAGATTACAGACTTCTTTCAATACCTCAAATCTT1340.1369764993304507No Hit
GAAAACATCCAGTTTATTTAAATTGCAGTTGTCGTAAAGTATGCATGAAGT1320.13493207396730963No Hit
ATTCAAGATCATACGATTCTCTTAAACAAGGGAGCATAAGATTCCGGAAAA1270.129821010559457No Hit
GTTAAGATTGTTCGATCTGAGAAGGTTAAGGATGAGATTATTCTTGAGGGA1260.12879879787788648No Hit
ATCCTAGAGGTAAAGTTTTTAGTCTTTAACCACTGAGTATGATGTTCACTG1220.12470994715160437No Hit
TAGTTTAGCATTGCCTTAAGATAGTAAATCCAGAAAACAACTGAATCAAGA1210.12368773447003382No Hit
TATCTGAATGATTAGATGAGTATAGTGTGAAGGTTAAACTACTGCTACATA1210.12368773447003382No Hit
GAATTATTACTTCGCTAGTCAATAAAACTTCTGGGAATGTCAAAGTATTTG1210.12368773447003382No Hit
GAGCATCATATTTGAAGACAATTCCATCCGAACTTGAAACATATGAAAAAC1200.1226655217884633No Hit
ACCTGATATATCGTTTTCGCTTTTATTTTCAGGTGTTCTGAAACTCATTTG1180.12062109642532225No Hit
GTTTCAAATGGGTTTCTCAATACATCAAGTAAGACAAACACACGCTGTGTT1170.11959888374375172No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCG1160.1185766710621812No Hit
ACATCTAAGGGTCTTAAGAGAAACAAGTGAGAAGAAAAAACAGTTACGGTT1150.11755445838061067No Hit
CATCTATTTAGGTAGAACCTTATTGGTTACCATTTCAAGGGATGATAGTTT1140.11653224569904014No Hit
AGATAATACATCTCTTCATGAATCGAGTTAACAGTATCAAGAAGACTCTTT1130.11551003301746962No Hit
AAAGAACAGAGACTAGACTAGATACAAAAGGTTCCTCAGCATTTGATTTAA1120.11448782033589909No Hit
TAATGATTGTTTGTCTTGGTATGGGTTTCTGTGTTCATTATCTACTGCTAT1110.11346560765432856No Hit
ATACAACGTAGAGAGTTGATTTTCACTTTTACTCATTTTACCATTCCCATC1110.11346560765432856No Hit
ATCAGATACATGATTTGCAAATACTTTGTCCTTGTCTGTTGGTTCTCTTTT1110.11346560765432856No Hit
GAATGGAATCATCTAATGAACTTGAAAGGAATCATCATCAAATGAAGTCAA1100.11244339497275804No Hit
CGTAAAGAGTTATGGTTTCTTGTTGCAAAATCGTTATTATGATTCCTAAGC1080.11039896960961697No Hit
GTCATTTACAGTGATTCTGATGCTCTTACAAAACGGGTTTCATCTTCAAGG1080.11039896960961697No Hit
GGTTAAAGGTAAAGGGATGGAAAAAGATCTATCAAACAAATGGAAAACAAA1070.10937675692804644No Hit
GTTTATGGCATGATAATCTTTCAATCCTGAATGCTAATAAAAATGCCAAGG1060.10835454424647592No Hit
AGAGATGTATGAAAACTCTTGTGTAAGAAGTCTGTTATAATCGGATATTTT1040.10631011888333486No Hit
AATGTATACAGTGTTTCCATAATAGATGCCGATGCTTCTAAATCATCAATA1040.10631011888333486No Hit
ACGTGAAATAGTGAAATTACCTTGGAAAACTTTTTACAAGCTTTGCGTAAA1040.10631011888333486No Hit
GGTGTGTACAGGAAACTTACAGGGAAAGATGTAGTTTTTCGAGTACCCAGT1030.10528790620176434No Hit
GTAAAAGAGGAAGAAGATAAAGGGAATCAGAAGAATGGTGGAGATGAACCT1020.10426569352019381No Hit
GTATATGACTAAGTGGAAGACTTCAAGTTGAAAAGGTTATTTGTTGTATGA1020.10426569352019381No Hit
GAGGAAAACAGAATTAAGGGTCCGTTATGAACAGAATTTGAAGGAAGCTGC1020.10426569352019381No Hit
GAACTGTTAACGAAGTTCCTGTAAAGCTGATTGAATTAAAAGCAGTTTGAG1010.10324348083862328No Hit
CATTAAAACATTACAACACTAAAAACAACAACAAGTACGATGAGTACGAGA990.10119905547548223No Hit
GTAGTTAAAGAGAAATGTTTCTTCCCACACTACTTAGTCAAACATAATGCT990.10119905547548223No Hit
ACTATGTTCCTTCTCTGAAACTGGATATTGTGGTTCGTTCTCTCGGATGTT980.1001768427939117No Hit
GTTTAATTAAGCAGTTTTTGATGGATTATGTAAACGCACTACTTGCATTCT980.1001768427939117No Hit
ATGGTATAGCCACTATTCCTGTATCTGCATTTTATCAATCAGGAGAAGATA980.1001768427939117No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTATC206.984396E-445.0225071
TCTGCAA351.1968586E-744.9994936
AACGTAC207.0020306E-444.9994916
ACAGTTC207.0020306E-444.9994937
GGTACAT207.0020306E-444.9994933
ACAAACG302.1452888E-644.9994913
AGTTTAC551.8189894E-1244.999493
GTTTAGC207.0020306E-444.999493
GGTCCGT207.0020306E-444.9994919
CCAATAC351.1968586E-744.999498
GAATCGT453.765308E-1044.9994922
TAAACGT207.0020306E-444.999494
AGCGGTC351.1968586E-744.9994924
TGGCAGA207.0020306E-444.9994935
ACTAGTC700.044.9994915
TTCTTAG207.0020306E-444.9994935
CTAGTCG700.044.9994916
TAATTCT453.765308E-1044.9994941
AGTGACT351.1968586E-744.9994919
TCATGTG207.0020306E-444.9994939
Produced by FastQC (version 0.11.2)