FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_62.351000000432f1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_62.351000000432f1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187937
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC76224.055614381415048No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT15960.8492207495064836No Hit
AGAAGATCCAACATAGCTTGCGTAAATGATCACTGTCTCTTATACACATCT7590.4038587398968803No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCG4650.24742333867200178No Hit
ATATAATTTCTCGCCTAAACATAAACAGATCTTTAGGTACGAACTTGAAAC4160.2213507718011887No Hit
AACAAGGACTGAATAGATATCATGATGACACTAAACTCATACTGTCTCTTA1990.10588654708758787No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACC206.9939037E-445.031694
TGACGTG253.874387E-545.007726
GAAGCGA302.1532378E-645.0077176
ACACCGC207.0122833E-445.0077176
CGACCCT207.0122833E-445.0077176
TCGCCTA600.044.99574311
CGAACGA253.8804843E-544.99574313
GCGAACG253.8804843E-544.99574312
GTCCTAT302.1571886E-644.99574313
AAGCGAG302.1571886E-644.9957437
GCTTGCG900.044.99574316
CGCCTAA600.044.99574312
CTACGTA302.1571886E-644.99574328
CTTGCGT900.044.99574317
TGCGAAC253.8804843E-544.99574311
TCTCGCC600.044.9957439
GAACGTC351.2055898E-744.99574318
CTCGCCT600.044.99574310
GCCTACG302.1571886E-644.99574326
CTCGAGG302.1571886E-644.99574318
Produced by FastQC (version 0.11.2)