FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_61.351000000432d7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_61.351000000432d7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172668
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC23301.3494104292630946No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT7010.4059814209928881No Hit
ATGGTAATGAGAAAAGTGTAAGAGTAGACTAGTCATACCTGTCTCTTATAC2790.16158176384738343No Hit
GAAGTAATGAAGTACTGATACATGCTACAAGATCTGTCTCTTATACACATC2750.15926517941946394No Hit
AAACAGCATACCTTAAGTAAGCCTATAAGCATTATTGGAACTGGTTTACAT2490.14420738063798735No Hit
CATCAATTCTGTCAATGTGTGAATACGTTAGAATAGTTTGTCGTGTGTTGT2440.141311650103088No Hit
CACAACAACAACAAACAAACAAACTCAGTCTAGTGTAAGTGAGAAAAGGAA2270.13146616628443025No Hit
ATAATCCCTGTCATTTACTTTACCATTGATGCCAATATTCCTGTCTCTTAT2240.1297287279634906No Hit
GTCTTTTGGAGTAAGTCATGTCTAAGAAGTAAGTGTATGTTTGTCTTTTGC2230.12914958185651076No Hit
TTGTTACACAAGAACAGACAAATTGGTACTTGATACAACAGAAAGTCTGTC2220.1285704357495309No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCG2220.1285704357495309No Hit
TGTTTTCAAGAAGCTTGTCTTAAGATATCTTCCTGTCTCTTATACACATCT2190.1268329974285913No Hit
GTAGTGTATGGTCTAAAACTCATACAAATGTCGATAGTGTTACAAAACAAG2150.1245164130006718No Hit
ATTCCATTCCATTCAGTTCCATTCCATTCCATTCCATTAAATTTCGTTCCA2100.12162068246577246No Hit
GAGTGATGTTATGGTAATATGCAAATCATTCCTTTGTATTCGAATAGCTCT2060.119304098037853No Hit
TACCAGTACAATCTTGAGATCATGGATGTTTATCATAGGAAATGGTATCTG2050.11872495193087312No Hit
TAAGACTACTGTAGAATAGTGCGTATAGGAATACAACCTGTCTCTTATACA2000.11582922139597378No Hit
AACTTGTTTTGTAACCTAATGACATCATCATCGATGACATCATCAGGTGAC1940.11235434475409456No Hit
ATTGTACTCTTCTTCTATCTTCTCCTTCTTCGCTATTGCTATTCGTTTCCT1910.11061690643315496No Hit
ATATGACACAAAGTCAATTACAAGTAATGAGTTACAGCAATAATACCTGTC1860.10772117589825561No Hit
ATATTCGTCATCTTGTTCCATACTTACTGTCATATCCCTGTCTCTTATACA1800.10424629925637639No Hit
GATGATACATACACACATACGAAGTAATGACTGTCTCTTATACACATCTCC1760.10192971482845692No Hit
AAATACAAGCTTAGCATATAGTTGAGCTCAACAAGTATCAGTTTTCTTCCT1750.10135056872147706No Hit
GAAATAGACTCAGTGTTTGGTAGAGTTTATGAAGATAGTAAGATGCAAAAG1740.10077142261449719No Hit
GTACTTCCTAGTAATGCCAGTAAGTTTGAGGACACATACATTGTATCTGTC1740.10077142261449719No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTGAC253.8596307E-545.0342062
TGTAGGG206.990393E-445.0342062
TAAGCGT206.990393E-445.0342064
CCTGACT206.990393E-445.0342063
TTACGTG207.0003857E-445.0211525
CGGGTGA207.0204056E-444.9950812
AGCGTAT207.0204056E-444.9950832
GCTGGGA207.0204056E-444.9950843
CCTAGCA207.0204056E-444.9950841
TCGGGTG207.0204056E-444.9950811
CGACAGC302.1563828E-644.9950814
TCTAGCC207.0204056E-444.995087
TCGGCCT207.0204056E-444.9950831
CCGTATG207.0204056E-444.995089
AATGCGA302.1563828E-644.9950810
AGTGCGT302.1563828E-644.9950818
CGCTCTA207.0204056E-444.9950811
ACAGGGA207.0204056E-444.9950813
GCCTGAT207.0204056E-444.9950834
GAGCGTA207.0204056E-444.9950831
Produced by FastQC (version 0.11.2)