FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_60.351000000432ca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_60.351000000432ca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124207
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC24441.967682980830388No Hit
ACAAACATAAGTCTCAGCCCTCAAAAATTTCCTGTTATGTCTTTGATTCTT3850.3099664270129703No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT2450.19725136264461743No Hit
AGATTTGGGAGAAATGTAGAGAAAGAGTCGTTGAAAGAATTACAAAACCGG2210.17792878018147126No Hit
TCCTACAATCATACCGGATGTACAGACAAATAATCATTCCACTATGACGTC1670.13445296963939232No Hit
ATTGTTAAGAACGGAGGTCTACGCAAGGAAGAAGATTATCCTTACTCTATG1440.1159354947788772No Hit
ATCATATTGAGTGATACTGAAGTTGGTCAAGATGATCGTTTGATTGTTGAT1380.11110484916309064No Hit
GAATTGAATGGAAAGGAACTCAATGGAATGGAATGGAATAGAGTGGAATGG1300.10466398834204191No Hit
TCAAAACACATACACACACAGAGAGACGAAGAAGAAGCAATCTAAGATTTT1300.10466398834204191No Hit
AAGATGGTGTAATTACTCACCCATCATTCACTTTACAAGCAAGTGAATTAG1300.10466398834204191No Hit
CATACATTTCTACTGAATCAGATCTGATCTGCTTTGTCAGTTTTTAAACTG1290.10385888073941082No Hit
ATGTAAGACATATGATGCATGAGAAGAAAGAGAAGATATTGAAGCAAGAAC1260.10144355793151755No Hit
AGATAGCTCCATATGACTTAGCCATTTTCAGTTTGGGATATTGTTATCCAA1250.10063845032888645No Hit
AAGTAGAAGATCAACAACAAGGGTACCATAAGGAGAACAATGAGATTTATG1250.10063845032888645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA351.1936027E-745.031831
TCTAGAG206.9845916E-445.031833
ACAGGCT206.9845916E-445.031834
CAGACAC206.9845916E-445.031835
ATCCTAG207.0123834E-444.9955737
TCACCCA207.0123834E-444.9955717
GGTGTCG207.0123834E-444.9955743
CATGCCA207.0123834E-444.9955730
CAGCAGT207.0123834E-444.9955725
TGGAACT207.0123834E-444.9955730
TAGCACG207.0123834E-444.9955722
GTAGCCA207.0123834E-444.9955723
TCTACGC207.0123834E-444.9955718
GAGGTCT207.0123834E-444.9955714
AGCATCG207.0123834E-444.9955730
GTGTCGA207.0123834E-444.9955744
CCATACG207.0123834E-444.9955740
CGGTGTC207.0123834E-444.9955742
GAACGGA253.8729246E-544.995579
GAACGCT207.0123834E-444.995579
Produced by FastQC (version 0.11.2)