FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_59.35100000043294.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_59.35100000043294.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154853
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC27351.7661911619406792No Hit
CTTTTAATTGACACTCTTTTCCACACTTTTCATCCATAGCATGTTAAGCAT4640.2996390124828063No Hit
ATGATAGTTTGATGGAACCCAAATAGCCAACGTAATCTTGAGAACGAAGAA4400.28414044287162665No Hit
CAAAAGAAACAGAGAAAATCTAGTTTGACTTTTATTGGCACACAAGAGAAG3680.23764473403808775No Hit
ATATCAAAGCCAATAAACGAAGCCTTAATCATTTTCTGTTTTTTTCGTGCT3670.23699896030428858No Hit
ACAAACATAAGTCTCAGCCCTCAAAAATTTCCTGTTATGTCTTTGATTCTT3500.226020806829703No Hit
CTGTAAGGAACACTAAACCCATTTCCATTTTCATGTTTTCTTGAAGATGTT3410.22020884322551063No Hit
CCAATATACAGATCACTTTTCAGTATTAATCTCACTTAGGGTACTAGTGAG3290.21245955841992084No Hit
GTACTTAAGAAGATTAAAAAACCAAAAGCAAGAAGAGTCATAGACTCAAAA3210.20729336854952762No Hit
GATTGATTGTAGGAAGAAGATCGTTTGGTAAAGGTTTGGTACAAATGCCTG3160.20406449988053188No Hit
CCTTTCTAAAGCAAGGAGAATAAGAAGTAAGGGCAAGAGGCATTTCTTCTT3050.1969609888087412No Hit
AGTTAATTTACCCTTCATATCATAATCACATCCATAAGTGTAGTGTATGAT3030.1956694413411429No Hit
GTATGGAAATTAGAAGAGGTTTTCTTACAAAGATCAAGGGGTAATATCCCA3010.1943778938735446No Hit
GTCTACATAGTCTACATAAACATGAGTCGTATAGTTACCAATGAGATTCTT3000.19373212013974545No Hit
ATATGTTTCTCTTGTCATATGGTTCGGACTTAAGATGCTCTCTGTTTTACT3000.19373212013974545No Hit
TATTGATGTAGCATCATTTTTACTCTTGCCCGCTGTTCATATGCATTAGTA2960.19114902520454882No Hit
TCCTAGTAGTGTCTGTTATGTTGTTGTTTCCTAATTTCAGTTTTAGTGTCT2760.1782335505285658No Hit
ACGTTGTATGATATCATTTGAACGGAATAACATAGATCAGGATACCCTTGT2760.1782335505285658No Hit
AATAACACCAACAGATTATTCGTACAACAGGAATTCAATGCACCATTTTTC2750.17758777679476664No Hit
GAAAAGACTCATGTGTGTTATACCAAGTGATAAGACTTACAATCCAATAAC2660.17177581319057428No Hit
ATATGCAACAATCTGGTTTTGATATCAGTGCTTATGCAGATGACAATAATG2640.17048426572297598No Hit
CATAATACCCATTTCAGTATGTCTATTTCCTCAAGTTATTAGTCCTACAAC2640.17048426572297598No Hit
AGGTATAGCATGCCTTAAATGAACGATGGTTAAAAGTATTCAACTTAAGTT2610.16854694452157853No Hit
GTTTGAATAAATGCACGTTCTAAATCGTTCATGAATGTTTCTGCTTCAATA2580.16660962332018106No Hit
TTAGTGCCTTACCAGATATGAGATTTGCAAATATTTTCTCATTCAGTAGGT2540.1640265283849845No Hit
AATAGCAGGTTGGTAAAGCAAAAACCAACAATATAAGATCCCAATTGAACA2510.16208920718358702No Hit
ACTGAAAACTCACTTTTACACTGTAAAAGTAGGATACTTTTACATGAAGGA2500.16144343344978787No Hit
GTACTGATGATGCTTATATCTTGCAAATACAAAAGCATTAGATCAAACAAA2500.16144343344978787No Hit
TTCTAATGCTTAAGGAATGTTTTAAATCTCCGACATGAAGAAGATGCTTTC2490.16079765971598872No Hit
GGATAAGCTTTTGACTTGTTTTGTTGTCATTGGTAAAATTGTTATTTTGCT2480.16015188598218957No Hit
ATACATAGTCTTAGTGTCTGGCTTCTTTCACTTGGCATAATGTTTTTGAGG2470.15950611224839042No Hit
ATGACATACCATGCATGCTGTGTGAATATGTGGAACATGAAGTTGATTCAA2420.15627724357939465No Hit
ATATGTTTCACTACCTGGACAATCCTAAAATACCTAAATCCACCAATGGAT2420.15627724357939465No Hit
GTATTATCTAGTCCCTTGGTGTATTTGACCACAATTTATCAACTAGATCCT2390.1543399223779972No Hit
GTATACCATACCAGAAAGAAAGGAAGATAGATGTTCTCATCAATCAATTCT2380.15369414864419806No Hit
GACATAGTAGTACTCATTTGAACAGGTGGTAAACAAAAAGCGATCTAACCT2370.15304837491039888No Hit
GGTAAGTATCAGAATCTTCTGGCATGTCATAGTTGAAAACGATGTTCACTC2360.15240260117659973No Hit
ATGTAAACATTCTCTTCCCTTGTCGACATCTTCGATTCAACAATCTAGAGC2350.15175682744280058No Hit
ACTATGAAGTATGAACAATGTATTTTCACCAGATGAGACAGATACTAAGTC2220.14336176890341162No Hit
CTTTTCGATTCGAATGTTTTCGAATCAATTCGATACTTTTTGATTCGATTC2210.14271599516961248No Hit
GAATTATAGTCCTGTGTACACAGGAAGAAAACAATAAAATGGGATTTGGTG2160.13948712650061673No Hit
AAGCACACTAGTCTAAAAGAGTTATACACAACACACAAAAGCACTTAGATC2150.13884135276681756No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT2150.13884135276681756No Hit
ACTCAGGGTTTAACCTCTATTGCATACAGATGCATGTTGATACGTTTTGTC2150.13884135276681756No Hit
GAATAGAATCGAATTGAATCAAATCGAAAAGAATCGAATGGAATCCAATCG2140.1381955790330184No Hit
GGATGGATGGAGAAATTGACATCCTCATCTGTGATACATTTGAGCTACTGG2130.13754980529921926No Hit
AGATAAACAAATACCTATGTGGAGAAAGTATTTCCACAAACACATATCCTA2130.13754980529921926No Hit
GTACCTTTGTAAACACAGATCTGGTCATGTTATTCCATTACTCAAAAACCT2100.1356124840978218No Hit
CTCTTAAGATTCAACAACATCACACTAAGATTAAAGTGCTCACAGAATACA2090.13496671036402266No Hit
AGAGATGTATGAAAACTCTTGTGTAAGAAGTCTGTTATGATCGGATATTTT2080.13432093663022351No Hit
GAATTATAGAGAGGCTTCATTTATGTTAAATCACATTCACATTCAAGCAAT2070.13367516289642434No Hit
AAATAAGCTTGTCGTTATTAGCATCCATACCTACCAGGTAACAACCGTTTA2050.13238361542882604No Hit
TGAACCTGATAATGGAATTGATACGGCTTATTATTCTGGACTTAGATTTGT2050.13238361542882604No Hit
TATTACAGATTCAGAGGACAACCCTTTAGTTTCAACATGATCAACCAATGA2040.1317378416950269No Hit
GTATAGAACTAAGTTTAGAAAAGCTATGAACCAAGCTGATAATGATGCAAG2040.1317378416950269No Hit
TAAATTGAAGACTCTTTTCCTGACTGATTAATAGTTACAGCTGTGCTTACC2010.12980052049362945No Hit
GAATGGAATGGAATGGAATTGTATGGAATGGAATGGAATGGTACGGAATAG1990.12850897302603112No Hit
ATTATCTGGACAAGACTACACCTCATTCAACTAACAGGTGGATCGGAACAC1990.12850897302603112No Hit
TTCTAGAACATAGGATTTAGGAGCTGAATTTTTAGGAGGAAGTGTAACTGA1980.12786319929223197No Hit
TGAGAAGATCATATGAAATAGAACACGACTCCTTTTTATTCCTACTGAAAA1970.12721742555843282No Hit
TATACACAATATGCTTTCATATGCATACTACACAAATGAGCACATAGGATG1940.1252801043570354No Hit
ATAGAGATCCTTGCGATTCGTTGTATCCGTAACATTTAACAATCTCGTTTT1930.12463433062323624No Hit
AGAATTACCACAGTTATCCAAGTAAACCATACGATCAAATGAACCATAACT1920.1239885568894371No Hit
TCATGTAATAGTTCCCTTGTGAGCAATTTAGAGACTTTAGAGAGCTTTCTC1910.12334278315563793No Hit
ATTGAGAAGATGTGAAATGGATTAGGAACATCTTGAAAACATTATCATCTC1900.12269700942183878No Hit
TTTAAGTACTTTGTGTCTTGTTATGTTTTTGAGTCTATGACTCTTCTTGCT1890.12205123568803963No Hit
GAATAATAGACTTACATCACGATATCTTTTTCTTCCTTATTCTGATTTTGG1890.12205123568803963No Hit
AAGTTGGTCTTTGAAAGGTGTTTACCAAAATTTGAGATATGTTTATCTTTG1890.12205123568803963No Hit
AAGAGGTACATTTAGTTCCACTTTTATTAAACCTCTCATAAAACACAAACC1880.12140546195424048No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATAG1870.12075968822044132No Hit
ATTTCACCCAAGTTACACAAGTGTGTCTAATAATAACAGAACTCAAACTCA1860.12011391448664217No Hit
GTACAATTTAAGAGTAGCAGATTAGAGAAAGACTCACAAACTAAATGATGA1830.11817659328524471No Hit
GTATTCATTCCCAGCTTAAGATGTGTTCATCTTCTTGGTGATGTGGTTTGT1820.11753081955144556No Hit
ATGTAACATTTCGAGCAATAATATTCGGGGAGGAAAGAAATGAAAATGAAC1820.11753081955144556No Hit
ATTAGATGATGACCCCTTTCATTTCCATTCAATGAGGATTCCATTCGGTTC1810.11688504581764642No Hit
CAATATAGTTTGAAGCTCTTTCCAGATCAGTACATAGAGAGCATCCTCATT1810.11688504581764642No Hit
AAATAGTCCTGTCTTTTTCTTTACGCTCTAAACTATCCTTAATCATTGAAA1800.11623927208384727No Hit
CAATCCAATGAAATCCAAAAGAATTGAATCGAATCGAATCGAAAAGATTCG1790.1155934983500481No Hit
CATACGTAGTGAGACAATGATTTGCTTAAATCCTGTCTGTTTCAAACATAG1790.1155934983500481No Hit
ATATTATAGGTCTGAACAACTTTATATGCCTTCTACCTTGATTCCATACCT1780.11494772461624896No Hit
GTACTTTAGCATTCCCATTTTACACCAAAAGAAGTGAAGCTAAGAACATTT1780.11494772461624896No Hit
CTTCTGTGGGTTTCCTAATTTTGATTTGAATGTCAGATGATTGGTATGGAA1770.11430195088244981No Hit
GATTAAGGTTTTATAAAGGGTCATAGTTGTTACACTTGCTCTTTCTTTCTC1760.11365617714865066No Hit
ACTAAAAGCATTGATTAACAAATTCAACATAACGAGATTTGGTTCAATTCC1750.1130104034148515No Hit
ACTCAGTGTGTCATTATTATGATCGGAAATGAGTTTTTCACTCAAGCTCCA1740.11236462968105235No Hit
CTTTTAGTTTCTCTTCTCAAACTTACTCTTGTCATGGTTTAAGTTTCTTAC1740.11236462968105235No Hit
TGTGAGAGTAGTGAATGAAATGTCAAATGTGTATGTGTGTGTGTAACTTGG1710.11042730847965489No Hit
CATCCCATTCTCTCTCTCTTTCTCTAATGGTTCTTAATCTGTTTTAGCATT1700.10978153474585574No Hit
AGAAAACACCAGTCATGGTTTTTAAGTCCATAGTGAAGCACTCAGTAAAAC1700.10978153474585574No Hit
TCATATAACGAGTGTCTCTACCTTCTTCTAACCCACTGAATTTCTTTCCTA1690.10913576101205659No Hit
TATTTTGCAATGTCGACTTATGAGATCGAATAATGAATAGACTCAAGATTC1690.10913576101205659No Hit
TGATTACGGTAAGCACATAAATGCTTGTCTTATTATCTATGGACCAACTAC1690.10913576101205659No Hit
AAGTATTTCTTCAGGTTTTGAACTTCTAATATGGTACTCATAATTGGTATG1680.10848998727825744No Hit
GTAGAGCAATTAATAGTGACGTTTTTCTTAACATCTAGCAACATTTCTGAA1680.10848998727825744No Hit
GTTCAAAGTAGTAACCAAGTACAACAAACAAACATCAATGTGACTCAAATT1680.10848998727825744No Hit
TTATTGGGTTATATCAAACAAGTGAAATGAGTTAGCATTCATTTGGTGACT1670.10784421354445828No Hit
TAGTAGTCCTGAATATACATCAAGCCTAAGTAAATCCCTATAAGCATACCA1670.10784421354445828No Hit
GATTAAAAAACCAAAAGCAAGAAGAGTCATAGACTCAAAAACATAACAAGA1670.10784421354445828No Hit
ATGTTCATCTTCTTTTTCTATTCCATCTCTGTTGACAGTTCTATTTCTGTC1660.10719843981065913No Hit
ACTTGTGTCAAAACACAAACAAGAAAAGTACTAATTGCAAGAAACAGTTAT1650.10655266607685998No Hit
ATTTTGATCTTTCTTTGATAAGTACAACAAAGGGAAGAATGTTGTGAAGTA1640.10590689234306085No Hit
GTTACAACTCAGAATTCTATTTCTCTAAGTTTGCTCATGTAAGCACTCATT1640.10590689234306085No Hit
TAGTGGGTTACATTGACCAGAAGGAGAGTAATCTGGGATAAAAGAAATCCT1640.10590689234306085No Hit
TCATTGCATATGGGAACAGATAGATAAGCAGATAAATAACTGTGTCTCTAT1630.1052611186092617No Hit
TTTGAATGGTTTCTGTTTTATTTCATGATCCTCTTGCTTTGAAGTACGATT1620.10461534487546255No Hit
TGTGATGATTCCATTCAATTCTATTCAATGATGTCTCCATTTGATTCCATT1620.10461534487546255No Hit
GAATACGATGTTGAAGAGTTTGTAGAAAAACAATTGGCAGAAGATGAATTT1610.10396957114166339No Hit
AATTAGTTGTAAGGATGAGTGAGAAAGAAATTGACTATGTACATCTGTCTC1610.10396957114166339No Hit
TGATCAGATAATAACGCAAACAGATTATTCGTACAACAGGAATTCAATGCA1590.10267802367406509No Hit
ACTCCATACAGATCAAACATCGGAATTGGTATTCTTCTATCATGAAACACA1590.10267802367406509No Hit
ATGTAAAGAGGATCAAAGAGTATATGGAGAAGGTTGAGTTAGAGCTTTCTA1590.10267802367406509No Hit
AATGTAGACTATTGGCTTCCTGTAAACGAGATTAATTCCGGGTATTTCAGT1570.10138647620646678No Hit
ATGTGATATCGCTTTCGAATATACATGATTTACATCTTCCAGATAAAGATA1570.10138647620646678No Hit
TGGATCTAGAGGATATAATGCTAAGTGAAATAAGTCAGAGAAAGGCAAATA1560.10074070247266763No Hit
CTTTGATCTTTATGAGTAATAATGGTGTCTTTTGCTTTCGGTTGTTCTTCC1560.10074070247266763No Hit
ATGCAGATGATACTATCCGTTGTTGAATTTGTCGATTAGAATTCTTTTTGG1560.10074070247266763No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCCGT206.9925655E-445.0284351
CTGCTTG253.875464E-544.99935528
GTGCACT207.014868E-444.99935535
GGAATGT253.875464E-544.99935514
AGCAACG207.014868E-444.99935526
CCTTACC207.014868E-444.9993557
TTGGCAC406.7502697E-944.99935535
AGTCCCC207.014868E-444.9993553
GGTACCA253.875464E-544.9993555
GTACCCC253.875464E-544.99935528
TGCCCGC406.7502697E-944.99935527
ACTGCCT207.014868E-444.99935524
CTCGTTG207.014868E-444.99935539
CTACTAT253.875464E-544.99935540
TACCATG502.1827873E-1144.9993557
CTCAAGC253.875464E-544.99935541
CGAGTGC207.014868E-444.99935544
CAACGGT207.014868E-444.99935528
CCGATCC207.014868E-444.99935533
GGCACAC406.7502697E-944.99935537
Produced by FastQC (version 0.11.2)