FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_57.35100000043251.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_57.35100000043251.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59952
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC37286.218307979717107No Hit
GTTTATTCTTATGATGGCAGATGTAGCTCTATTTTTACATGGTTAAGATGG8141.3577528689618361No Hit
GGTTTTGATTTAGGGTTAGGATTGCACCAAAAATAAGATCCACTTTACCAC7441.2409927942353884No Hit
GACTAGTGAAATCAAGAGCAAGTTTAGGATTGGATGTTTTGAGAAGAGCTA7071.179276754737123No Hit
ATGTTCATTCTCTCTTCCAATAGATAATCCAATATCTTCAGGATGTGATTT5950.9924606351748065No Hit
AACCAATATACTTGTGCTCTTTTAGGTTTTTTTGCTAAAATAGACTTGATG5030.8390045369629037No Hit
ATCAAATGGAGAAATACAATCCGATACAACAGATGACTCTATGTCTGATCA4680.7806244995996797No Hit
GTACATAGTTGATTCTTTTTCAGTTTTGTGTTTGGTATGGAAGATAACAGT4530.7556044835868695No Hit
ATATATGTCCTTACCAAGTTCTTTTTAGTTTTTCATTCTGTCATTCGTTTT4470.7455964771817455No Hit
AACTCAGACATTTGGAGATATGTTGTATTACCATGATTCTCAAGGTTGTCA4110.6855484387510008No Hit
CTACATTCACTGTAAATTCACCTGTACTGTTACCATTACAAAGAATTGTTT4010.6688684280757939No Hit
GTTGGTCTCTTAACAGAAGATCGTTTAAGAAGATACCAAACATAGTCCAAG4010.6688684280757939No Hit
CTCTTTAGCTTAATTAGATACCATTTGACAATTTTGGCTTTTGTTGCAATT3970.6621964238057112No Hit
ACATAAAACAGAGTCCTCACTTTCATTTCTCTCCAAGATAAACTTTAAGGT3570.5954763811048839No Hit
ATATAATCTCCAGACGATGATGAATCTAGAAGATGAAGAAACACACATCTT3560.5938083800373632No Hit
GATTAGACTTATTAGGCCATGGTTATTTCAAAACGTTTAACGTATACAAAA3540.5904723779023219No Hit
ATTATTATCATTCCACTGGAAGTACAAACTCATCACATGGCAGAGTTGCTA3100.5170803309314118No Hit
GTGTTTCAACAGTTTTTAAATGCAGACTTACAAATTATGGTAGGATCGGCA2960.4937283159861222No Hit
AACTTGGAATCCTAATCTTGTCATTCCTGTATCGATTTTGATGTGAACCTT2830.47204430210835335No Hit
AATGAATTCCACAGGTATGATGTGAAGATGAAAAAGTGGTGAAGGATAAAC2490.41533226581265015No Hit
GTTGTTTCCTGACTTTTTAATGATCTCCATTCTAACTGGTGTGAGATGATA2470.4119962636776087No Hit
ATGCTTGGAAGTTAAAATTGCAACTACACACATTGCTTGCAATTTTATGTT2440.40699226047504666No Hit
AAATACTAATGATGATGACGATGATCCTTGTTTTGTTCCTGACTTGAATGA2290.3819722444622365No Hit
GGAACATAAACAACAGAACATAACAAAGCCCAAACAATCTTAGTAATAATC2240.373632239124633No Hit
AGATAAAGTTATGGAAGTAAAACTGATCAATAGCAAAATCCTGTTGAAAGC2200.3669602348545503No Hit
GGTGATATGTATGACCTTTCTAGTCTTTTCCTTCAAATCTGTATTGTATTT2100.35028022417934346No Hit
ACCCAATTATTGCAATCTTCGTCTCCTTTTGTGTGTTCATTGGTACAAACT2080.3469442220443021No Hit
GTATAATGCGTTTAGATTTTCAGGGTCTGTTTTTAATACTTGTTCAAAGTA2080.3469442220443021No Hit
ACATATACTTGGACAAGCAATATATCATTTTCAGTAGTTTTTGTGAAAAGG2060.34360821990926077No Hit
TATCAATACGGCTTATGACAGAATAGTTAAGTCTGATGTTAAATATCGTTT1940.32359220709901254No Hit
AGTCAATAGTTAGAATAATCCTAATGACTACTGACTAATGACCATTGACCT1800.300240192153723No Hit
ATAGTAACTATGTTCCTTCTCTGAAACTGGATATTGTGGTTCGTTCTCTCG1790.2985721910862023No Hit
CATTTATTTCAACATGCTTGACAAAATAAGAGCTCAAAATCTATCTCTGAT1780.2969041900186816No Hit
TTTGAAAAGAGAGTGACCTCTTGAAATTTGCTAGGAATTACAGCATCAACA1760.29356818788364025No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT1750.29190018681611957No Hit
GAATAGAACGGAATTGTCATCGAATGGAATCGAATGGAATCATCTAATGGA1740.2902321857485989No Hit
AACGTAAAGAAGAAGAAGAATCATCATCTCTGGTTTGATATCAACAAAACC1690.28189218041099545No Hit
GTCAATCCATTTGATCCTTGTTTTATGAATTCTTAACCAGAATGCAATCTA1560.2602081665332266No Hit
ATTTAAAGTTTGCTTGTGTCCATTTGGACCATCTTCCTTATATTTGTTGGT1550.2585401654657059No Hit
CCTCAACATAATAAAGACCACATATGACAAACTCATAACAAACATCATATT1480.2468641579930611No Hit
CATATAACTGCTGTCTTTCTTGCTTTCTGTTTTGATACAAACATTCTACAA1460.24352815585801973No Hit
TTCCGATATTGACGCAAAACTTGAAGATGTGGAAAGAATCGAAAAGAAAAT1430.2385241526554577No Hit
ATTTGTAGGGTCTTATAAATTAAGCTTTCTCAACTACTTCAAGCCAAGTAA1430.2385241526554577No Hit
ATCTATACTTGAACCAAACATCTAATGCACCTCCTCAAATTGAATGTGATG1380.2301841473178543No Hit
GAATAGAATGTTACAAAGCTTTGATAAGTTCTTTGCATATAAGTAACTAGT1380.2301841473178543No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1290.2151721377101681No Hit
GTGTTACTTGCATTTATTTTTATGTCGTCTTTGTTTGTTCCACTGTATTGC1290.2151721377101681No Hit
GGTTATAATGAGAAAACTTTTCATATACGAGTTGACAGATGATAATAGCAC1250.20850013344008542No Hit
TATCACTTCTAAATGGGAGAATGAATGGGAAAACTCTTGGAAAATTTTGAG1230.20516413130504405No Hit
ATGTATTATAGGCTAGGAATACAGCAGTAGACAAAAATGATAAACCGTGTT1210.20182812917000267No Hit
CACTTTAACCACGTAATGTGAATGTTCAATGTAAATTTCAGCATTTGTTTT1200.20016012810248196No Hit
CGATGATATCATTCGATTCCATTTGATGATAATTCCATTTGATTCCATGCG1200.20016012810248196No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1190.1984921270349613No Hit
GTTTCAATATGATCTGTAAATTACAGCTTTCACTTTGCTTGTATATTTTCC1190.1984921270349613No Hit
GATCATAACATCAATATACATTTCTCTTCAAGTTCTTGTAACACAAATGTA1120.1868161195623165No Hit
ATACTACTACTACCTGTAATCTTTTTCCCTATCTTTTTTTTGGTCGTATTT1120.1868161195623165No Hit
GAAAAGCCAATAGAATGTGCTGGTTAACTTGATGATCAATAAAGTTTCAAA1110.18514811849479584No Hit
GGGAGGAGAAGATATCAAATTTATTGCACGAAGAATGTTGATATTAGCCAG1110.18514811849479584No Hit
CATTTATATCATCTAGAACCTGACAACTAATTTTTCCCTATGTAACTCTTG1090.18181211635975447No Hit
ATTTTGTCATCATCCAAACAAAATTTTTGTTGAGAGGTTCTGTCAACAGAA1070.1784761142247131No Hit
ACTTTATTCATTTAAATGGAGATGCAACAAAACTCAACTATGAAACAGATG1060.17680811315719241No Hit
TAGTAGCATGTTAGAGTTAAGCATAGTTAGAATAGTCTCCTGAAAGGTAAG1050.17514011208967173No Hit
AACAATGAGATCCCATCTCTTGATTTTGTTAATCTCTCTCTTAATCTTGAA1040.17347211102215104No Hit
GGAATAGAATGGAATGGAATGGAATCAACTCGAGTGGAATGAAATGTAATG1030.17180410995463039No Hit
GTATAGAACCAACCATTGTTCAAAATAGTACTAACCAAAAAACACCACAAA1020.1701361088871097No Hit
GAAATGATGATGAAGACGAAGAGAAAGAAACAATGGACATAGATCTCTAGT1010.168468107819589No Hit
GTACAATACAATTGCACAAAAGCCTTCCAGAGATATTACAACTCCAACAAC980.16346410461702696No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA970.16179610354950627No Hit
ATACATATGAATGCATATGTGGCCATCTGGAAAAAACTAGTAGAAATCACA950.15846010141446493No Hit
AAATCATTGAATGGACTCAAAAGGAATCATCATCGAATGGTATTGAACGGA930.15512409927942356No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA920.15345609821190287No Hit
ATCTTATATTCTCAGGTCTTCTATACTATACTGTGTGTTCTTTCAGGAAAA920.15345609821190287No Hit
CAAATCAGCAAAACCAAAAAGTGTATGTGCATCTTCCTTGATATGCAAAAA910.15178809714438218No Hit
GAAAATAGAGTTTTGTTGCCAAGAATCAAGAACAAACAAAGTATGTCTCTC890.1484520950093408No Hit
GTTTTAAAGATGAAGATTTTGAGTTCCAAAAATTAGGAGACTTACCCAAGA880.14678409394182013No Hit
CCTTTGAATTCCATTTGATGACAATTCCATTCAATACCAATTGATGATGTT880.14678409394182013No Hit
GTATTTAGAGGAAGCATTTCTTACAACAACACACAAAAAGCAAAAGATAAA870.14511609287429944No Hit
GAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA840.14011208967173738No Hit
CATTGTAAAAAAAACTGTGGTTGAAGAACATAGAAGAAGAGGTTGCTGTGG830.13844408860421673No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.13510808646917535No Hit
ATTTAAGAAAATGGGCTTTGATTGTGTTTATCCACCAAATACCCCAATCGA810.13510808646917535No Hit
TTCGTATATTCATGCATAAACCTGCAAATGAATCAGTATTTATCACGATTG800.13344008540165467No Hit
GGATGTTTTAACACTGAAAACAAAGGTAGAAAGAATCAACTTTTAAACTGA800.13344008540165467No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA800.13344008540165467No Hit
ATTAGCATCTTTCTTTCTCTCTGCCCCAAAAACTCCACATTCATTTACATA790.13177208433413398No Hit
CTTATGTTGATTGGACGGATAGTTTATTGAAAAAATGGGTGGCTGATGATA770.1284360821990926No Hit
CATTACATTAAGAGGTACATTTAGTTCCACTTTTATTAAACCTCTCATAAA770.1284360821990926No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCG770.1284360821990926No Hit
ACGATGAGTTGATTGAACTTTCTGAAGCGTTGAAAAGACAAATAGACAATT760.12676808113157192No Hit
GATCATAAGATTAAACAGAGAGATTAGAGATGGTTTTTAATATCATCCTCA750.12510008006405124No Hit
GAGATATTGGATCATGAGTGAGTGAAAAGAGCTGATATTACCGATTTTTAA750.12510008006405124No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA750.12510008006405124No Hit
AACAAGAAGAAGAAGAACAAGACAAGAAGAACAAAGTACCCAAGAAAAACA720.12009607686148918No Hit
AGATATTATGATTCCAGTCATGAACGGAAACTTTTTCACTGAATAATGAAG720.12009607686148918No Hit
ATCTACATCCAGTTTGAAATCGCTAATAAAGAAAAGATGATCCCATAATTT710.1184280757939685No Hit
AGATAAAGGTTTCTAAGTGAACTTATTATTTTGACTTCACTTGATCGATAT710.1184280757939685No Hit
CCAATAAAATGGGAAGGAAAAATCAAGAAGTGGAGTTTAAGAAAGAAGTGA690.11509207365892715No Hit
GTTCCACTGAAAAGATATACATCTTGTTGGACTATTCCAATATTATCTCTT680.11342407259140647No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.11342407259140647No Hit
CTATGATTGTTAACAAGTATAAGCTTAGAGGGAATGTTAAGAGTTTTAACC680.11342407259140647No Hit
ATCTTCACTTATCTTTTTACTTTGCTTTGCCAGTTCAAAAACTTTTTCTTG680.11342407259140647No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.11342407259140647No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA670.11175607152388578No Hit
ATTTTTTAACTCAAAATTGTGATTAAGGCTCACAACAGGTTCAAAAATGAG670.11175607152388578No Hit
CATGTTGTTACCTAGAACGAGTAGGAAATCTCATTGATGGTTGTGATCTTT660.1100880704563651No Hit
ACCATGAATGATATGTAAAGAGAAATACTCATTGGTTGGACATAACAATAA660.1100880704563651No Hit
GAAGACGAGGATGAAGAAGAGATATTGGATCATGAGTGAGTGAAAAGAGCT660.1100880704563651No Hit
ATTATTAGGCAATTTTCCTAACTCTACTTCTACAAGAGTTTTCCTCAATTA660.1100880704563651No Hit
GGACAAAAGAAAAAGTACAAGATACCCAAAAAAAGAGTGTAGAAACTACAT650.10842006938884441No Hit
GAGTTTTATCATCTTTTGTTGTCTTTTGGATTTTGGAAAGAGCGAGATCTC630.10508406725380304No Hit
CAAATATACTAATCCTACTTTGTCTAGTTTGTGTTTTGTGGTGATTTTATT630.10508406725380304No Hit
ACGTAAAGCTCTGTGATTTTCAACCAAACAGAAATTTGTACGCTTGAACAT620.10341606618628235No Hit
GGATAGTTTTGCTGGGTATAATATTCTTGGCTGATAGTTTTTTTTCTTCTC610.10174806511876167No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.10174806511876167No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.10008006405124098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGGT302.1311425E-645.00000435
TGATGGC800.045.00000412
CTTTTCA302.1311425E-645.00000417
GATGGCA800.045.00000413
ACTGTGC206.979856E-445.0000049
GTATGAT206.979856E-445.00000415
GTATGAC406.6302164E-945.0000049
TCCTCAC406.6302164E-945.00000414
AGCTCTA800.045.00000425
AATCCGA453.7107384E-1045.00000418
TAAATGC302.1311425E-645.00000417
CGATACA453.7107384E-1045.00000422
ATCTTCG206.979856E-445.00000415
TATACGA302.1311425E-645.00000424
ACTGATC453.7107384E-1045.00000422
ACATGGT800.045.00000437
TTCGTCT206.979856E-445.00000418
AAATTAT302.1311425E-645.00000432
TGGTAGG302.1311425E-645.00000438
CTCCTGA302.1311425E-645.00000437
Produced by FastQC (version 0.11.2)