FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_56.35100000043237.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_56.35100000043237.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49284
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC1020820.71260449638828No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG36247.353299245191137No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT29515.987744501258015No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGC1200.24348672997321646No Hit
CATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTC880.17855693531369207No Hit
GATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTC530.10753997240483729No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10300.044.1252245
AAACGCT250.00208785935.99918736
ATGCCGT13500.033.66590544
AACGCTG300.00510068129.99932137
ATTTGGT300.00510068129.9993213
TATGCCG17400.026.24940943
TCGTATG18000.026.12440940
ATCTCGT18050.026.05204637
TCTCGTA18050.026.05204638
TATCTCG18050.026.05204636
CGTATGC18100.025.9800841
CTCGTAT18100.025.9800839
GTATGCC17750.025.85857242
CTATCTC18150.025.78454235
CCTATCT18200.025.71370534
AACCTAT18250.025.64325532
TAACCTA18250.025.5199731
GACGATA18400.025.43420826
ACCTATC18400.025.43420833
ATAACCT18350.025.38090130
Produced by FastQC (version 0.11.2)