FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_55.3510000004321d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_55.3510000004321d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195831
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC101585.18712563383734No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT24201.2357594047929081No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG9600.49021860685999663No Hit
GTGTAATGTTAGTGTTTTGCTGTAGATGCTAGTTACTAACTTTGTATGACG4760.243066725901415No Hit
ATTGAAGTTTGTAAAGTCATTAAATGTCCTATCACTGTCTCTTATACACAT4120.2103854854440819No Hit
ATATAGTGTACCTTGATGAAACTAGCTATTCATCAAGGTTTATGGAAACAG4080.2083429079154986No Hit
CAATAAATCCATAAAAGTCAACCTCTATGGTTTTAAGGGTAAGAAATGTGT3550.1812787556617696No Hit
ACTTATTTCACCTTCTTTTAGTAACCCATCACTGGTAATATTCAAGCTGTC3490.1782148893688946No Hit
GAATAGAATCAAAAAGAATCGAATCGAATCAAATCGAAAACAATCAAATTG3440.17566166745816544No Hit
GTACAAATTATCTGCTTAAGAATACACTCTGAAATGTGGTCAGTGATTTTT3420.1746403786938738No Hit
TACTGTCACACTAAGTAGGATGTGATAAGTTACATGTCTGGTAATTCAGTG3380.17259780116529047No Hit
GTATCATTCTCAGTAGTAAATACAAGCTCATTAACATACTTCTTAGGCTGT3370.17208715678314465No Hit
GAAGAAAAAGAAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAACTGTCTCT3300.16851264610812383No Hit
TTCTACCATAACTGTACCATTCATGTTTTCAAGAAGCTTGTCTTAAGATAT3280.1674913573438322No Hit
ATCCAATACTGATTGTAGGTAGCATTTTGATTAAACTGCTGATTTTCGTAG3220.16442749105095722No Hit
ACTGTATGGTACAAAAGAGACATGTTTGTGATGTTTGACAACAACATTCCA3190.1628955579045197No Hit
GAATCGAATTGATTCGAAAAGAATCGAAATGAAACAAATCAGATCAAATGG3170.16187426914022807No Hit
AACATCATCAGCTCTTTTAACCTGTTAACCAGATAGTTACAGAAGTCTTCA3140.16034233599379058No Hit
TTTCGATTCAATTCGATTCTTTTCGATTCCATTAAATTTGATTCGATTCTT3120.1593210472294989No Hit
GGGTATTGGATACTTCCTATAATAGCTTCGTTTGCATCGTTTACTTTGAAA3050.1557465365544781No Hit
ATACGATGAATAACACTCGCATGAATTTTCAAACTTCTTTCAGATGACATA3050.1557465365544781No Hit
TCATTAAATCAGTTATGGTTCATTTGATCGTATGGTTTACTTCTGTCTCTT3040.15523589217233227No Hit
ATTGAAAACCATTTATGCCCGTATGTAGAAGAACTATTACTCCTGTCTCTT3030.15472524779018643No Hit
GTTAGTAACACATTCAATTATTGTTGTAAGTCCTAAGAGTTGTTTGTGTAT2980.15217202587945727No Hit
GGTGTTTAACCAATGTAATACCTGACTGTGTATTAGTACTGTCTCTTATAC2970.15166138149731145No Hit
ATTTTCAACTAGACTAGCCTTTATGCAGAAGAGAAATGAACATATTCTGTC2850.1455336489115615No Hit
CTAACTAATACTGGCTGTCAAAAGAATACATGAGGTTTTGAAAAGATGAGC2820.14400171576512402No Hit
AGTAACTAGTAGCTAGTAGCTAGAAACTATTTTCCCAAAGCCTTCTTTATC2810.14349107138297817No Hit
TATGTGTTCATCAAGGACAATTGCATGCACTTACAGAGGTAAGACATCAGT2810.14349107138297817No Hit
ATCTTAAGAATAATCATGACTCAGCTACACTATTCTTCCACTTTCTCATCT2800.14298042700083236No Hit
TATCCATACTGGTTCAAACATAATCACTCTATGCTAGGCACTATATAATTG2790.1424697826186865No Hit
GTTATTAGCATTGGTTATGGTGATATCTCAGAGAGTTAAGCTGTTAATCTG2770.14144849385439487No Hit
AGAGGAAACAGATAACCTAAAGAATGGGAGAAAATATTCCTGTCTCTTATA2760.14093784947224905No Hit
CTATTAGATACTGTTGTTACACGCGTTGAAAAAGGTAAAGAAGTAATTGAT2700.13787398317937405No Hit
ACTCGATTCTTTTCGATTCAATTCTTTTCGATTCAATGAAATACGATTCTA2680.13685269441508238No Hit
CTTTTTAACACTCATTCTTCACATGTCTATAGTATGGCACTAAAACACTCA2640.13481011688649908No Hit
ATACAGCTAACAAATTGCTTAGAGGGAAATTTATAGCTGTAAACACCTGTT2610.13327818374006156No Hit
AATTGATGCAAGCTAAAGACGAATATATTGAAAACCTGCAAAATGTATTAA2610.13327818374006156No Hit
ACCATTAATTGCTACTTTTACTGCCATTATAATCTGTCTCTTATACACATC2590.13225689497576992No Hit
AAACAATTCCGTATTCGATACGATTATTTTTTTAGTCGACCATCTATACCT2570.13123560621147828No Hit
ATGCATAACATTAAAGCCTATTGCAGCAATTAATCCACCTAAAATGATACA2570.13123560621147828No Hit
CATTAGGGTTAGAAAGAATATCAATATCAATCACCGAAAGAAGCTAACAGT2550.1302143174471866No Hit
GAATCGAAAAGAATCGAAATGAAACAAATCAGATCAAATGGAAAAGCATCA2550.1302143174471866No Hit
ATTGTAATTGTCAGACCGAAGACGATGTGATAAACACATTAAAAGATATCG2520.1286823843007491No Hit
ATACTCTTCCATTGTTTCTTTCTGAAAAACCAGACCAAATGTTGATATTTC2470.12612916239001995No Hit
AATATGGGTATAGCAATTTTGAAAACGGAGATCATTCAAAAAATACATCTC2460.12561851800787413No Hit
TTACTACACAATCTGTTAAATTTGACGATCTAAGTACCTGTCTCTTATACA2450.1251078736257283No Hit
CTTTGAAAACATCATGCTAAGTTACATAAGTGAGACACAAAAGGACAGATA2440.12459722924358248No Hit
GTATTAATGACCCTCTCTTTATTGACAAATCCTTTTTGTGCAGTAGCAACA2430.12408658486143666No Hit
CTGTATTTCTCAGGTTAAAAAAAAGACACAAAAGTTCTGGTGACATAAAGA2410.12306529609714498No Hit
GAATGTAAAAGTTCATCAAAGTACTTGTCACATAAATGTGAGTGTACTACT2390.12204400733285332No Hit
AGTTAGGATGGCTATTATCAGAAAGAAGAAAAACAAATGTTGGTGAGGTTA2380.1215333629507075No Hit
TTCTAACTGGAAATGTAGTTTTTCTACTAGGAATACCTGTCTCTTATACAC2380.1215333629507075No Hit
ACTTTATGCTAAGTGAAATAAACTAGTCACAGAAGGACAACTACTACATGA2350.12000142980427No Hit
ATTACGTACAAGTGTCCTAGTGCTCATGATGGTAAATTTAAATCCGTGTTG2320.11846949665783252No Hit
GCATAGTATTCCATGGTGTCTATGTACCACATTTTCTTTATCCAGTCTATC2300.11744820789354087No Hit
GTGTAATGGTAATGTGGTAAATGTTCCATTGATATTTTGAGTGCACATAGC2290.11693756351139502No Hit
GGTGATAACATGTACATGAAATTGGGGATCAATAAAATTCCTGAGTTGTTA2290.11693756351139502No Hit
GTTCTAACTTGAAATTAACACATTGCTAAAGCTAGAAAACTGAATATTCGT2270.11591627474710336No Hit
CATCGTATTGTTAAGTCAATTAATCAGAAAGCTCCTGATATTACACCTGTC2260.11540563036495755No Hit
TTTTTACTCCTTTCACTGAATGTCTTACACTAAATCCACAGTATCTGTAAG2260.11540563036495755No Hit
ACGAAAGATGTCATGATATCTTAACAACGCATCAAGAACAATTAACATTAA2240.11438434160066589No Hit
TGATTATTAAATGCCTTGAGATAGTCTTCTTTCTGTTAAAACTGCGTGTCA2240.11438434160066589No Hit
GAATTAATGTTACCATACCTTTTATCACTGATCTTTGTCAGTCACTCTAAG2220.11336305283637421No Hit
AAGTACTATGATAGTTAGCTCTTCTCTTCCTTTTCTTTATCTGTCTCTGTC2210.11285240845422839No Hit
GTAGTGTTTTTACTCTTCCTCCTAAAATAGATGGATAACCAGTTAAGCTTT2210.11285240845422839No Hit
ACTGCATAATATCCACCTAAATACTGACTTTGACTGGATAAATTTGGATCA2200.11234176407208256No Hit
TATTTCAACAGTAGTAGTGGTTGCTTCTTCTTCATCAGTAAGTGTGGGTTT2190.11183111968993674No Hit
TCATATCTACATCTTGAACGAACTTACCATCTCTAAGAAATACCTGTCTCT2170.11080983092564509No Hit
AAGCAACAAGGAAATTTTTGTACACGTTTCTGGCTTAAAAGAACAAATTAC2160.11029918654349924No Hit
CTATATGTTGTGTGCATATGCAAATCTCCATTGATATCATCTAATTCTGTC2140.10927789777920759No Hit
CATAAATTTAGTGGTGAGTTATTCACATGCATGTTTTTAAACTGTCTCTTA2120.10825660901491593No Hit
GAATAGAATCATCGCATGGAATCAAATGGAATTATCATCAAATGGAATCGA2110.1077459646327701No Hit
ATGTTAGATGGAAGAAAGTATCAATCCAACCATCAATATTGGTCCTGTTAC2110.1077459646327701No Hit
GTGTATTGGCAATTTATGCAAAAAATCGGAATTCATACAAACCAGCAAGTA2090.10672467586847842No Hit
ACATTACTGAACCTGAAAAGAAAACTGCATATTGGAGAGACATAAATGCTC2080.1062140314863326No Hit
TAATAGCCTCATTGAGTTGTAGTTCTCTATTTCCAATATTGCTCCCTAATC2070.10570338710418678No Hit
ATGGGTAGTACATTAAGAGGGTCTGAAAGTCATGTTCTCTATAAGACAACA2070.10570338710418678No Hit
ATTAGTATATGACGCTAAAGAGTTGTGGAAAGGCAAGAATACAGTAGAAGA2060.10519274272204095No Hit
AATGGATTCGAATCGAAACAAATTGAATCCAATCGAAAAGAATCTAATTGA2050.10468209833989511No Hit
ATACAAAGTCGTATATTTGCTGATTACTCTATTGCATCTTTTACTACTGGT2050.10468209833989511No Hit
GATCTATACAATGTAGATGGAAAAGATATCATCATTTTAGCTGAAGGTCTG2040.1041714539577493No Hit
TTGAACAGCAACTATGTTGTGAAGTATGAAGGTGTAAATCCTAATAATGGA2040.1041714539577493No Hit
GTGTTACTATCTCTGTTTTACAGATGAGGAGACTGAAATCATTACTGGTGT2020.10315016519345761No Hit
AATCTATCCATGACCAAATTAGGTTTTCTTCCTCAGAATACACTGTCTCTT2000.10212887642916596No Hit
TCCTTACCAACACTTGTTATTTCTTGTATTGTTGATTTTAGCCATTCTGAC1980.10110758766487431No Hit
GACAAATAGTATCTATTCTAACAGGATGCGTAACACGTATGTGTAATAGTA1970.10059694328272846No Hit
TAGCTTGACTGATTAGCTTCACTTCAATATTTAACCTTGATGTGTAGTAAC1970.10059694328272846No Hit
ATACACCAATGTGCAATTTAATGTGGGGAGATGGAAAAAGTGAAAATGTTA1970.10059694328272846No Hit
GGAAAAAAGGGAGACTGTTATGACCGATATTTGATTAGAGTTGAGGAAATG1970.10059694328272846No Hit
TCTCCATATTGTGCAAATAACTGTGTTTTGTTGCAGAAATGTTAATATGTT1970.10059694328272846No Hit
AAGTATCACGGCTACTACGATGAATATCAATCCGATTTTAGAGGCTCTTTC1960.10008629890058264No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAGGG206.988168E-445.0401345
TAGGGTT406.712071E-945.0401344
AGTAGAC206.988168E-445.0401343
AAAGTCG253.8585393E-545.040135
CGAATTG351.196604E-745.040135
AATTCCG253.8585393E-545.040135
CAAAGTC253.8585393E-545.040134
GGGTTAG406.726623E-945.028626
ACAACGA207.0234557E-444.99412521
CAGCGTT207.0234557E-444.99412518
AGACTGA351.206281E-744.99412530
ACACGCG502.1827873E-1144.99412519
CGAACAC207.0234557E-444.99412528
CCCTCTC600.044.99412511
TACTTCG253.88191E-544.9941257
GTCGACC253.88191E-544.99412535
CGAAATC253.88191E-544.99412527
CCTAGTG253.88191E-544.99412516
GGATGAC253.88191E-544.99412518
CCCTAAG207.0234557E-444.9941259
Produced by FastQC (version 0.11.2)