FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_54.351000000431f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_54.351000000431f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199654
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC166378.332915944584132No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT41962.1016358299858755No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG11490.5754956074008033No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC2780.1392408867340499No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC2500.12521662476083625No Hit
GAATGTAATGGAATGCAATGGAATCAAATGAACACGAATGGAATGGAATGT2190.10968976329049256No Hit
GAGTTATGTAGTGAGAAAAACAACTTCTTTTCACTGCTTGTCAGAAATAGT2140.10718543079527583No Hit
GGATTTAGACTTTAGGAATTGTCATCTTTTATGGTTTCAGTTCAGGATTTC2050.10267763230388573No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTAC207.0108066E-445.0110241
GCCCCTG207.019467E-444.9997531
TCGTTGT207.019467E-444.999756
TCGTTAC207.019467E-444.9997513
ATGGCGA207.019467E-444.999757
TGGACGA207.019467E-444.9997522
AGCCCCT207.019467E-444.9997530
ATGGACG207.019467E-444.9997521
GATCCTG253.8793223E-544.9997525
TCTTACG453.8198777E-1044.9997517
ATAGCGG207.019467E-444.9997544
GTCTAGT207.019467E-444.999756
CGACATG253.8793223E-544.9997519
GAGAGGT207.019467E-444.999753
CTGACTC207.019467E-444.9997527
TAACGCA207.019467E-444.999754
ATCACCG253.8793223E-544.9997513
TAGCCAG207.019467E-444.9997530
ACGATGC207.019467E-444.9997530
AAGCCCC207.019467E-444.9997529
Produced by FastQC (version 0.11.2)