FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_53.351000000431d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_53.351000000431d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76659
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC1623221.174291342177696No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT918311.979023989355458No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG46666.086695626084348No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTAT1850.24132848067415438No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGC1800.23480608930458263No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC1580.206107567278467No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC1340.17480008870452263No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCC1070.13957917530883523No Hit
GCATTGAGTAAATGTTCACTGTCTCTTATACACATCTCCGAGCCCACGAGA970.12653439256969176No Hit
GGCATTGAGTAAATGTTCACTGTCTCTTATACACATCTCCGAGCCCACGAG870.11348960983054827No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA780.10174930536531915No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC17650.044.8692745
GCAAAAT250.002095634435.9973919
GCTTCTG352.7972378E-432.1405327
AAAAAGT1500.030.0174061
ACAGCTT300.00511950329.9978268
AAAAGTA1550.029.0491032
ATGCCGT27400.028.98512544
TATGCCG32550.024.39915343
GTATGCC32600.024.3617342
CGTATGC32800.024.28177641
TCGTATG32800.024.28177640
CTCGTAT33050.024.03002739
TCTCGTA33150.023.95753938
TATCTCG33200.023.78592736
ATCTCGT33500.023.77439537
CCTATCT33400.023.57613434
CTATCTC33400.023.57613435
ACCTATC33650.023.53469733
AACCTAT33700.023.36625932
TAACCTA33800.023.29712731
Produced by FastQC (version 0.11.2)