FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_52.351000000431be.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_52.351000000431be.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80698
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC38184.731220104587474No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT4840.5997670326402141No Hit
CTTAATCCTTTGACACATCTACAAAATCACATAACGTTTGACCAAGTTTCA2230.27633894272472675No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG1610.19950928151874892No Hit
TAATTGAGTCCATTAAGTACTCCATGGACAATGTTTAAGCTGTACTTCCTC1560.193313341098912No Hit
AAACTCTTCTATCTGCAAAATGTAGGAAACCAATGTTAAATTTAGTGTCAA1330.16481201516766214No Hit
AAGTTAAACTCTGCTTCAAAAGCAACTTTAAGGAAATATTCATCCTGTCTC1310.1623336389997274No Hit
ATATACATCCATACACAATGGAATATTACTCAGCCATCAGAAAGAATGAAT1280.15861607474782524No Hit
ACTCCAATCTGTTAAAATGCATGTTGAATGTTGTTACTTGCAATTACCTCT1250.15489851049592307No Hit
TCATAACGGTATCGGAAATTCTCATAAGTCGTCTGGTTAGTACAGATGAGA1220.15118094624402093No Hit
TACAAGTGATGTGCATCTTTCTTAGTGATTAGCATGTATGTGGTCTTTACT1160.1437458177402166No Hit
AGTAACAACTTCACAAGAAACATCTACATCAGTAGTTGCTAAGAAAAGTAC1100.13631068923641232No Hit
ATCATCTTCTTCTTCATCTTATTCTTCTTCATCTTCTTCTTCTTCCACCTC1100.13631068923641232No Hit
TTACTACACTTATCTAAGTTACAGAGGAAACTAGCTTTCTTGTATGAAATG1090.1350715011524449No Hit
GATAAGAGTGTTATGTATGTCCCATGATACTTAATTCTCTTATCCACCCTA1080.13383231306847754No Hit
GAGATATTCTTCATACCATCAACACAAACCAAGGAAATAACAATCACTGTC1080.13383231306847754No Hit
ATTTTACATCGACTTTTTCGTCTGTTTTTTTATCTTCCTTCTTGTCTTCTT1070.13259312498451015No Hit
TTGTTATGTGATCTTTAGGATAATTCCCTTAGTTTCCATCACCAAAACCAT1070.13259312498451015No Hit
GAATTACAAAAAGCCGTATTCCCATTGATTACAGGATTAAGAAATACTGGC1060.13135393690054276No Hit
AGTATATTGTGTGATGTCATAATGTCAATAAGCTTCACGCACCAATGAACA1060.13135393690054276No Hit
AGGATACGCATAACGTTGTTTGTACAATTAAACCTGATACTCCACAACCAT1060.13135393690054276No Hit
GAATGATACTTTGTCTATTTCGGTTGGAAAAATTGTATTCAGAGATCGAAA1050.1301147488165754No Hit
TATTCGAACAGTGTTGATTACAACAAAAGAACAAGGTACGCGGTTATAAAC1050.1301147488165754No Hit
AAGAGGAATTGACCAAAAATGTCACAAAGATTGTTTTGGGGTGAGGAAAAT1030.1276363726486406No Hit
TGTAAGTGCTGTATAAAGCATTTCTCTTGACAGTAAAAAGCAAGGATTAGG1010.12515799648070583No Hit
GTATATCTCATACTCAAGCAAAACAAGTAGTCAATAGAAACTGTCATACTT1000.12391880839673845No Hit
GAAAAAAATGTACAAGGATGCGACAGTTCACTTTGAAATGTAACAAGATAC1000.12391880839673845No Hit
GAAAAGCACAATGTAAAATGTAAGATGGTAAAAAATGTCTGACTGTATCAA970.12020124414483631No Hit
GTTGTTGTTTTGTTCTTTGGGTTTTTTGGTTTTGCTTGTTTGTTTGTTTGT960.11896205606086892No Hit
TACCAATACAGAGAATGACGAAACCAGAGTGACAGAAGAAGTATTTCAAAT960.11896205606086892No Hit
AATGTAGACTTATAGAAACCACAGGTACTTCGAAAAAGAGTGGAACACAAA950.11772286797690154No Hit
CTGCTAATCAGTATGCTTTTGAGAATCATACTCACGATTTTCCAAAAATCA950.11772286797690154No Hit
AACCCTTTATCAGATATGTCATTTGCAAATATCTTCTTCCATTTTGTTGCC950.11772286797690154No Hit
CTATATGATTGTTCTCATTACTACCACTACTATTCCTAAAGCATCTGACAG950.11772286797690154No Hit
GTCATGATACGTTCATAATTGAACCCCTTATCATACATGCTCAATCTAAAG940.11648367989293415No Hit
ATCTGTGCTTACATAAAAGGTATAGCTACTGTCTTTTTGTAACACCAAAAA940.11648367989293415No Hit
CAATTATGTGTATCACAACATGAAAGATTTTAAACAAACACGTTTGTTCAA930.11524449180896677No Hit
AGTATTACCAGCAATTTCCTAATGGATATAGGAAGCAAGAGGTAGTTCTAA920.11400530372499937No Hit
TAAGAACATACTGTGGTTTTCTCAGTGTATCCAAAATGTTATTTCAACATG920.11400530372499937No Hit
ATCGTACTGTGTGTCATTCAATACGATTGAAACCTACAAAATACGGTATTT920.11400530372499937No Hit
GCATACTTGAAAATCATAAATCATTTTCGCCCTTTTGTTCCTGCACAAAAA910.11276611564103199No Hit
TCTTTTACCTTGTCGAAATTATTTGTCATGATAATGTCTTCTAAGGCTGTC900.1115269275570646No Hit
AATATACAGTTGCATAAGCACATCCAAGATATATCATCTTCATCACTGTGT900.1115269275570646No Hit
TTTTCTTTGTTTACCACAACAGCTACTACTTGGAGGTTAAGAAACCAACAA890.11028773947309722No Hit
GTATGAATACACAAGGGTAAAAGGAGGTCTTGAATCCGCTGTTCGTATTTC890.11028773947309722No Hit
ATATTGGGTAGCAACAAGTAATTGTTGCGTTAAATTGTCAAGACTGTTTAA890.11028773947309722No Hit
TGTTAATTGTATCTAAGGTACTACACCACACTTGTTGTCAATATATGCTGT880.10904855138912985No Hit
GAGAAAGAGAAAGAAAGACAAGCAATACTAAACAGCAGCAAATGTACATTA880.10904855138912985No Hit
GTGATATCATTTGTTCTTAGTACTCTCCATTGACTGTGAAAATAGTCTAAA880.10904855138912985No Hit
ATGTATCCTGTGTATTTGCTCCACATCAAGAGATCACTACAACTACATTGT870.10780936330516246No Hit
AACATTCTGAAATGAAAAGTCCACAGGTATTTTGTTCACCACAGTACATGA870.10780936330516246No Hit
ACCAATCAAGATTGATAACTCTTGATCTTTCAGACAACAAAATTGAAGGAG860.10657017522119508No Hit
GTTTACAGAGAATATTCTGACGACTTCAACTTTCTTACCTAATTTACCTTC860.10657017522119508No Hit
AAGCAATACGCTTATCAATATGTACGCTTTACAAATCAAGAAAATCAAAGC850.10533098713722769No Hit
ACATTAGTATCTGTTTCGCTAATGGAAAAAAAATCCAAAGATTGTCACTGT850.10533098713722769No Hit
GAAATGGGTATTGATAAAAAAGTCATCAAGGAAGGAAATGGAACAACTTTC830.1028526109692929No Hit
ATTCAACACCAAAGATGGTTCATTAAAATTAGTACCGCTGTTTGATATGAG830.1028526109692929No Hit
TAATTGTACCGTAACGATTAGCTATAGTACTTACTCAGGAAACAAAATAGA830.1028526109692929No Hit
TCTCAGTACACTTCTTTTCATACCTCAAGAGACTAAAACTAGTTTGAGGAG820.10161342288532553No Hit
GCAATATATGGTGATTTGTTTAATCATTCTAAGGGAGTGATTAATGAATCA810.10037423480135814No Hit
GGATACAAGAATATTAATCCACATACCACTTGAACTTTCATGTTTAACAGT810.10037423480135814No Hit
CAGAATGTTATGAAGAGCAACTAAATCATGTAGGTAATAAAGTACTTAGCA810.10037423480135814No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTAA206.952569E-445.0546261
GTATATC206.952569E-445.0546261
GAGCTAT253.8309394E-545.0546231
ACAACCC206.9952465E-444.9987636
CTGTCGG206.9952465E-444.998767
AATCCGC206.9952465E-444.9987633
CTGTCCC206.9952465E-444.998769
TCCGCTG206.9952465E-444.9987635
ACTGACA253.8591534E-544.9987641
ACGTTAG253.8591534E-544.9987622
TAGACGT253.8591534E-544.9987619
GACGTTA253.8591534E-544.9987621
CCCAGTT206.9952465E-444.9987637
CCAATCT206.9952465E-444.998764
CTCCCAG206.9952465E-444.9987635
CGTCTGG502.1827873E-1144.9987630
TAAGTCG502.1827873E-1144.9987625
CGCTGTT206.9952465E-444.9987637
GGATAGT206.9952465E-444.998766
CGCGAAT206.9952465E-444.9987622
Produced by FastQC (version 0.11.2)