FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_51.35100000043195.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_51.35100000043195.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52150
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC34066.5311601150527325No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT12242.3470757430488973No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG3050.5848513902205177No Hit
TGCATACCTAGTGTCTAGAAGAATAGTACCTAACACATTAGAGTCTGTCTC860.16490891658676896No Hit
GTTAGAATACGTTCCTGTAACAATATGACTAAGGTACATTCATGTAACGAT850.1629913710450623No Hit
TTTGTGGTATATCACTTCCTACACTGTGTAGAATCTGTCTCTTATACACAT840.1610738255033557No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGGAGAAGAAGAAGAAGAA710.1361457334611697No Hit
GAATGGAATGGAATCAAATGGAAAGGAAAGAAATGGAATGGAATGCAATGG670.12847555129434324No Hit
ATCATATGGTTGGTAGTCAACAATTTGAATTTCACATTGTTGGTATCAAGA650.12464046021093002No Hit
ACTTTCTTTCACTTAGCATAATGCTTTCAAGACTCATCTACGTTGTACTAC640.12272291466922339No Hit
AGTCTAGATGGTAAACACTGTTAGCAATTTAAGTGACTATCCTCTAACTGT600.11505273250239692No Hit
TGAAATCAGTATGAGTAGTTATGAAACGAAAAGGACAGATAAATCCCTGTC580.1112176414189837No Hit
TTTGTGTACCTTTGAAATACATAATTGCTGCATAGTATGGTAAATGTAGCC580.1112176414189837No Hit
GATCATATCACTGAACTGGGTAAGAATTTCTAGAACTAACGGATAAACTCT570.10930009587727708No Hit
ATCATAACGGTGTAATCAAAAGTAGCGATATAGATTCAAAGTCACGTACCT570.10930009587727708No Hit
CAATACCAGTGTCAGTAACATGAAGAACATTGTTTTCTTTGTCAACCTTCT570.10930009587727708No Hit
TCTTCAATCTTAGCTTCTCCGAATACAATGTAAGTATCAGATACCTGTCTC560.10738255033557045No Hit
ATTCAATTCTTTCGATTCGATTTGATTTGATTCTTTTCGATTCGATTCCTG550.10546500479386386No Hit
GTAAACATTTCTGTTTGATGCTAATTTCACTTCCAATGTTTAAGCCCTGTC550.10546500479386386No Hit
ATGTAACATCTTATTAGGCACAGTGAAGCATTAAGATTTGATCTGTCTCTT550.10546500479386386No Hit
TTGTAACACTTGTTGTTATGATACTTGGAGTATATGCCTGTCTCTTATACA550.10546500479386386No Hit
AAAGTGATGACAACAATGATGATGAAGCTTTACAACTACATAGTCTGTCTC550.10546500479386386No Hit
CTATCAGGTGTCAGAAATGTTCTTGGTATTTCTCACTTGGTGTAGAACTTC530.10162991371045062No Hit
GTCATTCAGTATTAAGATTCTGAGATTCACACACATTGTTGTACCATAGAT530.10162991371045062No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGTC206.97145E-445.00000421
ACCACCA206.97145E-445.00000415
TTGAGAC206.97145E-445.00000433
CTGTGAC206.97145E-445.00000439
TACGAAA206.97145E-445.00000429
GACTGTG206.97145E-445.00000437
CGAAAAC206.97145E-445.00000431
CATCGAT206.97145E-445.00000419
TGCCGTC4200.044.46428745
CAAGAGA301.1250474E-437.50000428
TGTGACT250.002088756336.040
TGACTTC250.002088756336.042
GGCAATT250.002088756336.07
TATGTCC250.002088756336.022
AACTTCA250.002088756336.030
ATAGACT250.002088756336.06
GTGACTT250.002088756336.041
CTTGATT250.002088756336.014
CTGTTCC250.002088756336.031
ACGAAAA250.002088756336.030
Produced by FastQC (version 0.11.2)