FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_5.35100000042bd1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_5.35100000042bd1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110046
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC43573.9592534031223305RNA PCR Primer, Index 27 (95% over 21bp)
GAGATGAAGAGTTTGATAAAAAGGATAAAGGGACATGGATAGTTCATTCTG20071.8237827817458154No Hit
CTATATAGGAAGACATAGTGATTAAGATGTTAGGTTTTCTAAACTGTTGCA11291.0259346091634407No Hit
ATGTTAAGATATCACAATAAGTGCTCTGTTTACTTGGAAACAATTTTTAGG11221.019573632844447No Hit
ACTCAGAATAGCTTGAAACTGGAAATAACCCAAATGTCTATCAAATGATAA8620.78330879813896No Hit
TAGTTATGATGGCGATAATAACATTTGCTTGTACTTTTGTGGGATTCATTT8490.7714955564036857No Hit
GGATACAACAAAACAAAAAGCAAACAAACGAAAAACAATCAACAACAATAA7370.6697199352997838No Hit
AGTATGTACAGGAAATTAAAGTTTTGTTTGAGACTTCATAAGCTTGGTGCA6700.6088363048179852No Hit
ATATTACATTTGAGTCTATCGAAGCCTATGAAAACTTCTTTAGTGAAGGAA6240.5670356032931683No Hit
GAATAATATCAACATTCTAGTCCTCTTTTATTCAACTAGCTGTTGATACTT6110.555222361557894No Hit
TAATAGGGTTATTATCAGATGGTGGTGTACATGCACATATGAACCATATAA6020.5470439634334733No Hit
AAATAAAGCGTTGAATCCTAGATTTCCAAGAAAGGCAATGAATGTGAAGAG5900.5361394326009123No Hit
AATCTATTTATCAATCAGGTTATTGCGGCAAAAAAGTATAATTTCAATGAG5810.5279610344764917No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGT5060.4598077167729859No Hit
GTTCAGTAATTCTATTGAGCATTATGGAAAAGTCATCACAGATAGTGAATT4840.43981607691329083No Hit
ATATATAGCTGAGAACTGTAAAACAATTTTATCGATTTACCATCATAACAA4710.42800283517801646No Hit
TCTATACACTAACAATGAGACTGAAGAAAGAGAAATTAAGGAGTCAATCCC4640.42164185885902256No Hit
AAGCTATACAGTATTCCAAATGTTTGCAAGTTTAAAGGATAACAGAACTAA4640.42164185885902256No Hit
CTTTTACATTGTAGTTAAAAGCTTGAACAAACAACTATTACACCTCCTTAA4620.4198244370535958No Hit
GTATAATATTATGTCCAGCATTGATATAGGTTAATTCCTGACGGGTAAAAT4590.4170983043454556No Hit
GCATATAGATGGATCTTGGTTTTTGTTTTTTGTTTTTTAATCCATTCTGAT4490.4080111953183215No Hit
ATCGTATGCAAGAGGAAAAAGATCATGCTGTTAAACAAAAAACAGAACCTA4230.3843847118477728No Hit
TTGGTAAAGAAATATGTCACATACATAATGCTAGTGTTAGGGGTAGAAAGT4200.3816585791396325No Hit
GGAATAATACTAAATGTCCATCTAGAGAAAACTGAATAAACTATCCCCAGA4120.37438889191792524No Hit
CGTATACGTTACAGAATAACTACTTGGTTTTAGAAAGAGGAAGTGCTCAAG3760.34167529942024244No Hit
ATAGTATTCCATTTCATGAATATGCCACATTTTGTTTATCCATTCATCATT3660.33258819039310833No Hit
ATAAATATGAGTCCCATGATATTTGACTGTTGCTTTCCTTGTAATGAATGT3650.3316794794903949No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3310.30078330879813897No Hit
ATCCTAAAAGATTACCTGTGTATGTTCTGTAGCAAAGAGATTCTTGATTTT3280.2980571760899987No Hit
CAGTAGTACTGTTCTATGATGCAAAAGAGTTATGGAAAAATAGAAACAGGG3220.29260491067371824No Hit
AGGTATAAGATTACCAAACAAATAACTCAGAATAATCGTCATAAAGATGCT3120.28351780164658413No Hit
ACCTATATCCTGATAATTAGCAGTTGCAATACTTGGATCATAAGCGATCTC3070.2789742471330171No Hit
TTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACCTTTTGCATAATGAA3070.2789742471330171No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2960.2689784272031696No Hit
GATATACACTCAAGATGTATTAGAATTCGAAGTTATGGTGCAAACATTAGA2930.26625229449502935No Hit
TGCTTATACTGTGTAAAGCTTTCTATTAATTCGGGGTGATGTAATACTTCT2930.26625229449502935No Hit
GTACACAATTACCGTATCATTCATAGAAAAACCAATTACCGTTAATACCGC2900.26352616178688915No Hit
TAAAAGCTCTCAATTTTAAATGACTTTAATTCGAAAACAAAAACCCTTTGC2900.26352616178688915No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA2870.2608000290787489No Hit
ATAGAATTTATCTACGTACTTTTGCGTAGTTCTGCTATTTTTTATTTTTGA2690.2444432328299075No Hit
TTATCACTTTGTGCTTTTGTCATCAATAACTTTTTTTCCCCCCTAGGATTC2660.24171710012176728No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2630.23899096741362702No Hit
AAATATGAAAGAAAAACGTAGTAATGTTCGTTGGTATTTTGCAATAGGGTT2570.23353870199734655No Hit
CAAATATACCCACCAATAAAAACATATCTTCTGACGAAAGCAACATAAACG2560.23262999109463314No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2480.22536030387292588No Hit
AATATGAATACCCCTGTCATTTACGATACATGTTTTGATAACATCCTTACC2450.22263417116478562No Hit
ATATAGTAAAAAGGCAATGCAGATTATGTAACATGTAATTACAGTTAGGCT2440.2217254602620722No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2410.218999327553932No Hit
ATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGG2390.21718190574850516No Hit
TAAAAGATGTGAAGAAACCTTAAATGCATTTTACTAAATGAAAGAAGCCAA2350.21354706213765154No Hit
CTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTATAA2330.2117296403322247No Hit
AAATAAGTGATTCTAAAGAAGGTATGGGAACCAGCACAGTTGAATTAATCT2300.20900350762408448No Hit
TATACACACAATGTGATACCATTCAGCCATAACAAAATAATGAAATCCTGT2230.2026425313050906No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2200.19991639859695035No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2190.199007687694237No Hit
CAAGAGATTAAAAGAAATCAATTGCTATAGAGGAATTAGACATAAAAGAGG2130.1935554222779565No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2120.19264671137524308No Hit
GAATATATGTTGAAGTTTTACAACTCCATAAGACAAATAACTCACAACAAA2100.19082928956981626No Hit
ACATTACCAAACATATGATTCAGCCTTCTAGGAAAGTTTTGGAAATACCCT2080.18901186776438944No Hit
GATTGACATTATGACAAAACAATATTAGCATAAACTGAGGACTTCTGTTTA2050.1862857350562492No Hit
CTAAAGAAGAAACATGCTGATGTCATTATATATCCCTCACTCTGATGAAAG2030.18446831325082239No Hit
AAGTGGAAGAAAGGATATCAGTGATTGTAGGTAAAGTGAATAAAATGAAGC2000.18174218054268215No Hit
GTGAATGTATAGTTTACTACGTCACCCACTTTTTCAACTTCCTTAACATCA1990.18083346963996874No Hit
ATTAATACTAGGCATGAAAAGCGAATTAATTGTTGGTAAAAACGAAGTAAC1980.17992475873725533No Hit
TATTGATACCGCTTACTTTATGTTAGGTTTGGAGAAAGTGGATCCTGATTA1960.1781073369318285No Hit
GAAGTAATCATATGTGTTCTTGGGTTGTAATAAGTGTTTGTAGAGAGTAGG1960.1781073369318285No Hit
TTGATAAATTTCAACACTGGATTTATGAACACCCAAATCATACATTGGAAG1950.1771986260291151No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA1890.17174636061283463No Hit
TTTCTTATTAGATAGAATCTAGGTGGAACTGTCAATCAAATTCCTTGTACT1880.17083764971012122No Hit
TACTTAATGTATTTACTTTCCATCTCTTTACCATTTGTCATGGCAACAATT1870.1699289388074078No Hit
ACTGTAGTGTTTTAAAGGTGTATCTAAGAAATTTTCTACGCCGCTAAATTC1860.1690202279046944No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCG1840.16720280609926758No Hit
GTGTAATTGTATAAATAACAGTCATTGACAACAAAAAATGCATACACAAAC1800.16356796248841393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1800.16356796248841393No Hit
GTTTAGAAAATATCAAGATGGATAAATTTCATGTCTTGGAAGTTTGTTGTT1800.16356796248841393No Hit
GAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1790.16265925158570052No Hit
TTATGTTATTTTGCTATGAACCAGCAATAAGAAGAAATAGACTCTTCAGTT1770.1608418297802737No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1760.1599331188775603No Hit
ATATAGATTTAAAGTCACTTCACATGATGATGATGATGATGATGATGATGA1740.15811569707213347No Hit
GAAACACTTGAATATGGCTTTATTGATGAAATTATGGCCAACAATTCATTG1720.15629827526670664No Hit
GTATAAGTATCCTTACCGCATTTAATGGATTACTGATGTTATTTCTGGGGA1710.15538956436399323No Hit
TATCAACAAACTCCTTTTCCATTTTATATCTTCCTTCCAAAATTTTTGCAA1690.15357214255856644No Hit
TATTAGAGAGAAACAAAAATCTTCATTAATTGCAACTTATGTGATTTCCTA1650.14993729894771277No Hit
ACGTATACTAGGTTTTTTTTTTTTTTTTTCGCAGCTAGTGTAAATGGAATT1620.14721116623957253No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1620.14721116623957253No Hit
CTTATAGCTTCTTCAGACAATTCGGTATAGTTTCCTTTATCTAAATACCAG1590.1444850335314323No Hit
GTTATGTTGATTTCAGGTATACAATACAGTGATTCAACAATTCTATGCATT1550.14085018992057866No Hit
TTCTTAGTCTCTACTTGATGAGACATTGTCATCATACTTTACTTCATTTAG1530.13903276811515183No Hit
TTTTTACACAATGTACAATTCCTTTCCATCAAGATCTTCATTAGCTTATGA1520.13812405721243842No Hit
ATATAGTGCTCAAATTTTTCTCACAAAAACCTTTTCTTCACTTTCTCATAT1510.13721534630972504No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA1490.1353979245042982No Hit
ATAAAAGGGAATACACGAAATACTAAATTCTCATCCTTAAACAAGTATCTG1490.1353979245042982No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1450.13176308089344457No Hit
ATATCTAACTGATCACAAAGAATTTGGTATAAGATTCCGTTAATAAGCGGA1440.13085436999073113No Hit
GTGGCATTTCTATACACTAACAATGAGACTGAAGAAAGAGAAATTAAGGAG1430.12994565908801772No Hit
ATTCTGTAAGATAATTCTTAAAGCCTTTACATGCAAATTTTTTGAAGCACA1420.12903694818530434No Hit
GAATCGTTTTGTAACCATGTTTATTGGTATCATTGATTTTACCCGTCAGGA1380.1254021045744507No Hit
TTTTCAATCTTATTCTCTACAGACGCCATTAGCACATTATCATTCAAGTTC1370.12449339367173727No Hit
CAGTAGTACTGTTCTATGATTCAAAAGAGTTATGGAAAAATAGAAACAGGG1360.12358468276902387No Hit
TTTTAAAGCATCATTTATTCCTTTGCTCTGTCTTTTTTTAAGCTTTTATTT1330.12085855006088364No Hit
TGTCCATAACAATAGATATTACTTGGTAGTTATTGACCGAATAGATGCCCC1330.12085855006088364No Hit
TTAGAATAATGCTTGTATTTCTGGATTGGCATCGATTTCTATTGAGACTGG1330.12085855006088364No Hit
TTCTTGTTCTACTTGAATCTAATTTTTCCATTCGTTCTAGAAGTTCTTAAC1330.12085855006088364No Hit
GTATTAAATACACACTATGATGGGAGATTTCTATTCTATCTTGAAACTGTC1280.11631499554731657No Hit
ACGTATACTAGGTTTTTTTTTTTTTTTTCGCAGCTAGTGTAAATGGAATTG1280.11631499554731657No Hit
GTACGCATTTATTGCTATAAACTTCCCTGTTAGAACTGGTTTTCCTGTATT1280.11631499554731657No Hit
GTTTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTG1260.11449757374188975No Hit
ATAATAAACTACTCAAGGTTACTGGAGAGATTAAATGAGTAATACATCTGT1250.11358886283917634No Hit
TGAGAGCTTTTTACAATTTTACAACAAAAATTGCAGGAATACAAGAGGTAA1230.11177144103374952No Hit
AAGGTAAGAGAAGTATTTAGAAGACGTTTGAATGCCTTATTGATAGCGCCA1230.11177144103374952No Hit
ACAATATTTAGCTAGTAAGAACCATGTCAAATAACTACTTTCTTCTGGAAT1220.1108627301310361No Hit
GTCATAGTTACATAACTTCGTTTAACATCAATTACACCCCACAGTTTCACA1220.1108627301310361No Hit
ATAAAATCTGCTATTTTATAAGAGAGGCACAGAAATAGGCTTCACTAATTG1210.10995401922832271No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1200.1090453083256093No Hit
GGTATCATAGATATCGTTAAACAAATGGGAGGAAACATTGAATTAAGTAAT1180.10722788652018247No Hit
ATTTTCAACCAGTCACATAATTCACCAATAAAACGATCTTTATTTTGTTCT1150.10450175381204224No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA1140.10359304290932883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAG302.1475826E-645.00000433
GATAATA900.045.00000414
AGTCATC453.783498E-1045.00000431
AGGAGTC302.1475826E-645.00000439
ATGGCGA900.045.0000049
GAGTCAA302.1475826E-645.00000441
GGACTTC302.1475826E-645.00000439
CAGTTGA302.1475826E-645.00000437
CTATAGG600.045.0000041
TATGGGA302.1475826E-645.00000423
CAGCACA302.1475826E-645.00000432
TGGCGAT900.045.00000410
TATATAG1800.045.0000042
TATGACA302.1475826E-645.00000410
GACTTCT302.1475826E-645.00000440
GCTCTCA453.783498E-1045.0000046
GGAAGAC1200.045.0000048
GCACAGT302.1475826E-645.00000434
ATGAGTC302.1475826E-645.0000047
AATGACT453.783498E-1045.00000419
Produced by FastQC (version 0.11.2)