FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_49.35100000043152.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_49.35100000043152.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132784
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC1579811.89751777322569No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT95247.172550909748162No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG15531.1695686227256297No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC3850.28994457163513676No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC3070.23120255452464153No Hit
AAAAAGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACG2190.16492950957946742No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA1430.10769369803590795No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCTAC206.9980155E-445.0165821
CAGGTTG207.0110237E-444.99962622
GACAGGT207.0110237E-444.99962620
AGGACAG207.0110237E-444.99962618
ACAGGTT207.0110237E-444.99962621
GTGCTAG207.0110237E-444.99962611
ATGAGCC207.0110237E-444.99962626
GATGCGT207.0110237E-444.99962627
GAGCCTA207.0110237E-444.99962628
GATGCCT207.0110237E-444.9996264
ATGGTGC207.0110237E-444.9996268
GTCCCTG207.0110237E-444.99962637
CGATGAC207.0110237E-444.9996265
GGACAGG207.0110237E-444.99962619
GCACAGT253.8723185E-544.99962225
TTGGTCA253.8723185E-544.99962219
TAGCTGC253.8723185E-544.99962222
AAGTCGC253.8723185E-544.9996226
TGCCGTC16650.044.59422345
GATACGA356.190945E-638.585641
Produced by FastQC (version 0.11.2)