H23LNBCXX_l02n01_bir_1228_pcontrolb_48.35100000043138.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l02n01_bir_1228_pcontrolb_48.35100000043138.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22315
Sequences flagged as poor quality	0
Sequence length	51
%GC	41

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC	837	3.7508402419896933	RNA PCR Primer, Index 46 (95% over 21bp)
TGTAAACTGTAATGAACATCTGTGTAGTTAAATCTCACCTACCCCCTTAAT	96	0.4302038987228322	No Hit
CACAAACTCATATTGAGAGACTTTCTACATGATACCTTACCAGTACTGCAC	89	0.398834864440959	No Hit
AATGTGTATAGTGCTGATTTACTCAAGAATGAAACAGGTAAATATGACATC	76	0.3405780864889088	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT	63	0.2823213085368586	No Hit
CTGTAGAGATACCAAGCTTATGAACTATTCTCCCAAAAACAGATTTGTTCG	63	0.2823213085368586	No Hit
ATGTCTATTTCATAGGTTGCTCTAAGGAATAAATACATACATGCAATGCCT	62	0.27784001792516244	No Hit
AATGATAATTGGTTGATTTTTTCTCCAAATGATTGGAGTGTTACTCCTAAT	61	0.2733587273134663	No Hit
ACATTAATGTAATGGAATCGGATGGAATGGAATTGAATGTATTGGAGTCGA	60	0.2688774367017701	No Hit
TTATAGGAGTTCCCTTGTAATGACAAGTTCTTTTTCTCTTGCTGCTTTGAA	56	0.2509522742549854	No Hit
ACATGAATATTTTTGGCTCTAACAACTGCACCTTTGAAATGGCATGAAACG	55	0.2464709836432893	No Hit
CCAAATAATACAACGAGTGCAATACCAAATAGTATCCACATAATGCGTATT	55	0.2464709836432893	No Hit
TGATAGAGGTGAGTCTTAAATTCAAATTTTCTGGCTACCAATTCCATATTA	55	0.2464709836432893	No Hit
ATGATAATCAACAAATACGCAAACTTTCTCTAGAAGGAGCAGAATTACAAC	53	0.23750840241989693	No Hit
AGAATATTTTCGTTTCTGTCGCATTTTAGCTTGTAAATTGAGTACTTTCAT	51	0.2285458211965046	No Hit
GGAGAAATGGAAATGATCTTTAGGACACTGTTAATGTGCATAAAATTCTGG	50	0.2240645305848084	No Hit
ATACTACAAGGCTACAGTAACCAAAACAGAATGGTACTGCAAAAACAGACA	50	0.2240645305848084	No Hit
GTATTGTCGTGAAAAATTCCTCTGAAAGAAACAGCTTTTTCTTTTTCATAA	48	0.2151019493614161	No Hit
ATTTTATCATCTGTCTCAGTTCTCTATTGGCTTTGCTTGATTGATTTTTCT	47	0.2106206587497199	No Hit
CATGCATACAGAATGTACTTGGCATTTTCTAAGAGAGTAGACCTCCTCACC	46	0.20613936813802372	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC	46	0.20613936813802372	No Hit
TTCTTCTTCTTCTTCTTCTTCTTCTTCTGCTTCTTCTTCTTCTGCTTCTTC	44	0.19717678691463142	No Hit
ATTACAGAGTGGGAACATTTTAGTAAAGGCATTGGGATGTTTAAATGCCAA	43	0.19269549630293525	No Hit
GTATCATTCATAGAAAAACCAATTACCGTTAATACCGCGGCAATAAAATGC	43	0.19269549630293525	No Hit
AAATGTAAACTCATGGGTTTTTACATGATTACTAAGGGTATTGGCGTAAAA	43	0.19269549630293525	No Hit
GTTAGTTTCTTATGGAAGTCTTACAGCATTTTGTGTTCTCACCCAGGATAA	41	0.1837329150795429	No Hit
ATGCTGTAGAAGAAAAAATTGAAATTGCAAAACGACATTTAATACCCAAGC	40	0.17925162446784673	No Hit
TTGCTGAATATGTTGAACTGCTTCATTGATATCATCGGTTACCAAAACCAG	39	0.17477033385615057	No Hit
AACTCCAACAGTTTTCTTGTGGATTAAACCATTGTATCTGAAACTGTTCTT	38	0.1702890432444544	No Hit
GTGAAAAGAATCGAATCGAATCGAAAGAATCAAATCGAAAAAAAAATCGAA	38	0.1702890432444544	No Hit
TATAAACACAGACCAGAAAGCTGAGAAATACTCTTGATAAATTCTTCATCT	38	0.1702890432444544	No Hit
TAGTAGATATCACCTTTAAAGCGCTTTAGCTTACTTGTAGAATCGAAGTTA	37	0.16580775263275824	No Hit
AATCTACTCTCAGCAATTTTCAAGTATATATCACGTTGCTGTTAACTCTAG	37	0.16580775263275824	No Hit
ACCATATCATGTGTAAACAGAAGTAATTTTACCTCTTTGTTTCCAGTTTCC	36	0.16132646202106207	No Hit
TAAATTGCGGATTGGTATTGATATCGGCATGTACTTCCATATCAACATGAT	36	0.16132646202106207	No Hit
ATCATATACCACAACATTTGCAACAAGTGAACGCTGTGAAAAATCAAACAC	35	0.1568451714093659	No Hit
TTAGAAATTGAACAACATGGAGCAGAAAAAGTAGGATTTGAAGAATTGATT	35	0.1568451714093659	No Hit
ACATAACCCTTGTTGACTTTTTCAAGGGAATTAAATAGCTACTCCTTTTCA	34	0.15236388079766974	No Hit
ATAATTCCATTCGATTCCATTTGAGGATAATTCCATGTGAGTCCATTCGAT	34	0.15236388079766974	No Hit
ATCCGTTGTTGTATTTGTAACACCAAAATCCCATAGATACTTATCAACGGA	33	0.14788259018597355	No Hit
TTTCGATTCGATTCTTTTCGATTCGATTGATTCTTTTCGTTTCGATTAGAT	33	0.14788259018597355	No Hit
GAATGAACGATCCACAATACTGTAACAAACATATGAAACCTGAGCTTTATA	33	0.14788259018597355	No Hit
GGTTTTGGAGGTAATCTTCGAAAAGAAGTAGTGAAAACAAAAAAGTGGTAT	33	0.14788259018597355	No Hit
GAAGTATTCAGTGGGATGACATAGTGGAGTTCATCTCCCTAATGAATCAAA	33	0.14788259018597355	No Hit
GGAGTAAACATTGCAACTAGCCTAAAAAATCAAGGCTTTGAAGATCTAAAC	32	0.14340129957427739	No Hit
GAATAAGCATAAGGATCTCTTGAAATGTCATCTATTATCCCAGACTGTGCT	31	0.13892000896258122	No Hit
GTTGTAAGCAATAGGTTTTCCATTTTTTACAACAACATTCAAATTCTGATT	30	0.13443871835088506	No Hit
TTATAACAACTGTTTCTGCAGCAATCATGGAATTGATTGATACATCTATTG	29	0.1299574277391889	No Hit
GTACATATTCTTTGTGTGAAATTCATTGCAAAAGAATGGCTCTAATTCTTC	29	0.1299574277391889	No Hit
AAATGACTAGAAGGAAAACCTCACCTCAAAAGAAAAAATCAGAAACAGTCC	29	0.1299574277391889	No Hit
GATATACGTGCTTGTGCAATAATTTTCTCACCAATTTCTGTTGGTACTATT	29	0.1299574277391889	No Hit
GACAAAGCAATTCTAAAAAAGAAAAACAAAACTGGAGGCATCACAATTCTG	29	0.1299574277391889	No Hit
AATGTGATGTCTCCAAATTTGTTCTTTTTGCTTAGTATTGCTTTGGCTATC	29	0.1299574277391889	No Hit
AGGTATAGTCTACATAATGCTAGCCTTCTTCTAACATCTTCTCCTACCTGA	29	0.1299574277391889	No Hit
TTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCT	27	0.12099484651579655	RNA PCR Primer, Index 18 (95% over 21bp)
AACAGACATTGTTTTTGTGAAATGGAAACTCGAGATTCTAATCATAGACAC	27	0.12099484651579655	No Hit
GCTTTAATTTAGGTCTTTGAGTTATGTAAGCAGAATGCAATTAAAGAAGAA	27	0.12099484651579655	No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27	0.12099484651579655	No Hit
GAATAGACAATTCTCAAAAGAAAATGAACAAATGGCCAACAAACATATGAA	26	0.1165135559041004	No Hit
AACTATTGATGCAAATGTGTTGCTAAAATTAAACCGCAATTTTATGAAAAA	26	0.1165135559041004	No Hit
GTATAAAGGAAAACAAGCTATTTCCAGTACCGCTATTCAGCAAACATTAGT	26	0.1165135559041004	No Hit
TCATTACATTGGTAAACTTGTTTGAGTGAGTATTGAGTTGTGCATCATTTC	26	0.1165135559041004	No Hit
GTGTATAAAATAAGCAGTAAATGTGACAGGATTACATAGAAGTAAGTGTTG	26	0.1165135559041004	No Hit
AACTCCAATTGGAAGATTAGTTACCCGTAATATTTCGGATATCGAAACTTT	25	0.1120322652924042	No Hit
CTATACATCTGACAAACGACTGATATCCAGAATCTACAACGTACTCAAACA	25	0.1120322652924042	No Hit
ATATTACCCTAACGCATGGCTATAATGGTCAATTAAGTATGAATAGCTTTA	25	0.1120322652924042	No Hit
ATTGTTGATAGTATGTGATTGTCAGAAATCAACTGCTTCTCCTTTTTCAAT	25	0.1120322652924042	No Hit
GATTCATTTTTTCTGACTTGATTTAAAGTCCTCCATAAATTCTTCTCACAT	25	0.1120322652924042	No Hit
TAACTTCAACATACATCCAAAATCAACATACTGGATCAAGTTCACAATTAA	25	0.1120322652924042	No Hit
GAATAGAATAGAATGGAATCATCAAACGGAATCGAATGGAATCATCGTCGA	25	0.1120322652924042	No Hit
GAAAATATGGATGCACCTAAAAACAATCCATTTAAGCAAGAGGATATGAGT	24	0.10755097468070805	No Hit
TCCATGTACAAATTGGGGTTAATTATGGAAACTACCTCGTTCAAGACTACT	24	0.10755097468070805	No Hit
TTGCATTGTTTGATTTTGACCTTTACAAAAAATGACAACAGTTTATAGTCT	24	0.10755097468070805	No Hit
GTATTTTAGAAGGATGCCTGTAATCTCCATTTGCACAAAATAAACTGACAT	24	0.10755097468070805	No Hit
TAAGATATGAGGACTACGTGAAAGTAATTTTTTAAAACGCTCTTCCGAAGA	24	0.10755097468070805	No Hit
CTACTATTAGCAGTGAGAATTCTTTGAATTTGAAAGTTAGATCGTAGTCAT	24	0.10755097468070805	No Hit
ATCAAAGCTTACTTGAAATAATCCGTTACTTGGATTTGGTGTAACTTTTAA	23	0.10306968406901186	No Hit
AAATAGAACTTGGCTACTCCTTGAAAATCTGTTTTTCCTCAGGACATTTCT	23	0.10306968406901186	No Hit
AGTTTAAGTTCTTTGTAGATTCCGGATATTAGCCCTTTGTCAGATGGGTAG	23	0.10306968406901186	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGTAA	20	6.885365E-4	45.000004	6
GTTCTCT	20	6.885365E-4	45.000004	19
TCAGTTC	20	6.885365E-4	45.000004	16
CACCTAC	20	6.885365E-4	45.000004	36
TGTAGTT	20	6.885365E-4	45.000004	23
GTCTCAG	20	6.885365E-4	45.000004	13
ACCCCCT	20	6.885365E-4	45.000004	41
TATCATC	20	6.885365E-4	45.000004	5
GAACATC	20	6.885365E-4	45.000004	14
TCACCTA	20	6.885365E-4	45.000004	35
CCCCCTT	20	6.885365E-4	45.000004	42
CCCCTTA	20	6.885365E-4	45.000004	43
TACCCCC	20	6.885365E-4	45.000004	40
TCATCTG	20	6.885365E-4	45.000004	7
ACCTACC	20	6.885365E-4	45.000004	37
CTCACCT	20	6.885365E-4	45.000004	34
CCCTTAA	20	6.885365E-4	45.000004	44
AGTTCTC	20	6.885365E-4	45.000004	18
TGCCGTC	100	0.0	45.0	45
ATTGGCT	25	3.768743E-5	45.0	26

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content