FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_47.3510000004312b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_47.3510000004312b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256632
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC101223.9441690825773867RNA PCR Primer, Index 46 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT12360.4816234920041148No Hit
GAAGTGTTATGATGTACGATTTAAACACAGGAAGAGAAACAGTTGTAGGAA7690.29965086193459894No Hit
ATTGTATCACATTCCAGTTTGTAGAGTAGATCATTTGATCTGTCTCTTATA6910.26925714641977616No Hit
TTGCAGTGTAATGTTGTATGTATAACCTTTGTTGGTATTGGTCATCAAAAT6080.23691511580784938No Hit
ATCTTGGATGGTTTCCATTAGCTACTATGTTATCAATGCTCTGAGTATCTA5700.22210792106985877No Hit
TACCGTATGTTTCTATAACTTGTGAAAGCACATCATTGATACGCTCAGAAT5510.21470432370086348No Hit
GAACAAATGATCTTCTTGTCTTCTGATGTCGTTTTAACTTTGGAGAAACTT5410.21080769350665546No Hit
ATAGTAGACAAACTGTAATGTAATCACTGAATGGACTCTTACGTTTCAGTG5260.20496274821534335No Hit
TCTCTGAGTTTAAATGACAGCATACTTTCTTGGAGTTGAAACATCCATTAT5120.19950746594345212No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG5010.19522117272982323No Hit
CTAATTGACTTACATGAGAAGAAGCAGGGTAAAAAACTTTTTAGTCAAGCA4930.1921038685744568No Hit
AAATAGCATGCTTTACAATGTCACTTACACAACATTGGAGTTTTTGATCAT4860.18937622743851118No Hit
GTATTTCTCAAGTGAGAGCAATAAGATTTTTTCTACTCACATTTGCTTTGG4850.18898656441909037No Hit
ACAAAGTTCATCCAAAGAGTAAGCATTTTGCTAAGAAAAGTTTACAAACCT4730.1843106081860407No Hit
GAATACTCTTACACGTTGCATTACTTGTCATTACACATGTAATTGAATCTG4710.1835312821471991No Hit
TACAAAAGCACTTAGAACTGAAACATTAAGCACGAAATCAATTTTAGAAGC4640.18080364101125346No Hit
ATCACAAGTATGGCATGTCTTCTGTAATTTCTCATAAACATGGGTGTCATC4630.18041397799183267No Hit
GTAATGAATAGAAGCTTGTTGTGTTACTCTAACATGATCATTTGAACCACT4540.1769070108170454No Hit
ATGTACAGTTGTTCTCATAAGAAACGGTTTTTAGTTTTACAAGGATTTGGA4470.1741793696810998No Hit
CAAATAGCATGTGTGATTTTTTTCAGTGTTGTTTTTATCGTATCGTAGTTC4380.17067240250631255No Hit
GTATTGACTAGGCAGTTAAATCAAGTTTTAGAGACTGAATGTTACTTAGCT4350.16950341344805012No Hit
TACCTAAATCCACCAATGGATTAGAATCATTTTTCGGACATTTGAAAAACC4320.1683344243897877No Hit
ATACAATACTACCTTAACCTTTCTAATGATCTGATACCTGTCTCTTATACA4280.1667757723121045No Hit
TCTTTACCCAGATACTCATTCCTCTGTCACCTAACTTCTTGAAATTTCATT4270.1663861092926837No Hit
AGTGTATTCATTACTTCATCCGTTGATAAGACAGTTGCTACTTTTAATGTA4210.16404813117615885No Hit
GAATGGAATCGCTTACTAAGAAAGGTAGATTTTGATTGGATGGATCAATCA4160.16209981607905485No Hit
ATGCTATTCCTATCATACTACCAATGACATTCTTCACAGAACTAGAAGAAA4120.1605411640013716No Hit
ATATGCAACATATCTCCGATCTCTTCATGACTATACCCCTCAATGACACTT4100.15976183796253No Hit
CTTGAAAAATAGGTTTCTTCGCTTTTCTGAACAAAGGGTATCTCTTCATTT4080.1589825119236884No Hit
GTGTTAATCAGAGTGCTTTTTGTTAACTGCCAGGTTTTGGTTATATACCTC4040.15742385984600518No Hit
ATCACACACACCTCTCCTTTTACTCGTAAGTGTACATCTTGTAAACATGTT3970.15469621871005954No Hit
AGTATGGTGTGTACAAAAGGAACATAACCATTATTTGCAAGTCTTTGCAAT3970.15469621871005954No Hit
TAATTGAACCTTTCGATTGATTGAATACATTAATGCAGCATTCATCAATAA3930.15313756663237632No Hit
CTCTAATGTTTCTTCTTTTTTAGCAAAACAGAAACGAAGCACTTTATTATC3890.1515789145546931No Hit
TATGTATCCCATTGATGTATCCCATTATAACAGTCAGAGCAACTAGATCTG3850.1500202624770099No Hit
GTATTGGGTATTTGTTTAGGAATGCAGTTGATGTGTAGCTATAGTGAAGAA3800.14807194737990584No Hit
AGGTTGGTTCATACAAAAGTACAGCTACTGTAACTGTTTCACAAATACAAA3790.14768228436048506No Hit
ACTAATCCTCTTGTTAATGAGCAATCAATATTAACCACAACTGGTTCTGTC3780.14729262134106424No Hit
ATTGAATCTGGCTTAGATATTCCTAATGCCAATACCATCATCATCAATTCA3710.1445649802051186No Hit
CTGTAACAACATCTGTAATGATCTGCATACAATGCCATACATTCACATTTC3700.14417531718569782No Hit
ATACTGATTACAATCCTTGTATACATTGGCATACAGCTGTCTCTTATACAC3670.14300632812743538No Hit
ATTTATGAGGCAATAGTTATGACAAAGGCATAGTAACATAACTGATTTCCC3650.1422270020885938No Hit
AGCTATTCCAGTTAAAACAGCTACATGGGGATATAGAAAATGAAACTTCGG3630.14144767604975217No Hit
ATAGTACAACTTTAATTCTGCTTGTTGATGGTGTTAATGTGTATGAGAGCC3630.14144767604975217No Hit
GTACAGTAGGGGTTTGTGTTAGTACACTATGATATTCACACGATTATGAAA3630.14144767604975217No Hit
CCTATGTCTATGTCTACATCTATATCTACATCGAGTTCTATTTCTACTCCA3580.13949936095264814No Hit
GTATAAACACAACACACGACAAACTATTCTAACGTATTCACACATTGACAG3560.13872003491380652No Hit
GAGTTAAGTTTATACTCACAGCATTTTTACTACGGACGATATGATATCTGT3490.1359923937778609No Hit
CTTTCTCTAACTGCTTCTGAAACAGAGTCCTAGACACATCTACTCCTTGAG3460.1348234047195985No Hit
AGATTGGGTGATGATATAAGAAGGATACCAATCCACAATGAAGACCTAACG3450.1344337417001777No Hit
AGATATGACATGTACTTGGTAAAGGAACTGATAATATAAAGCAGTGGCTTA3420.13326475264191529No Hit
TTCTTCACTAGTACCTACATTTTGTCAGAAACTGCGATATTTTTCAGACTG3410.13287508962249445No Hit
ATTCAAATCATTGGATGGATATATTCATGCAACCGATATACAATTGGCTGG3370.13131643754481123No Hit
ATGGATTCCTATTGATCACATGGATTGAGCTATAACTTTGTACATGTACTG3340.13014744848654886No Hit
ATTCATCACTTCGTAATTCTCAGCTTTTACATCTTCTACACGGATATTACC3310.12897845942828642No Hit
CTTTAATAAGCTCTTCTTGTCTACTTTCAAGTCCAAGTTTTTCAGATTCTT3310.12897845942828642No Hit
CATATATGATCATACCGAAGCAATGATTCTGTTAGATGTTGATTTTCAGCA3300.1285887964088656No Hit
GAACAAGTGTTCTTCTTTGTCAAATGCTTTTTCTGCATCTATTGAGATGAT3300.1285887964088656No Hit
GCATACAACACAGTGTTAAGCATGGATTCATAGAAGAGACAATATCCCATC3240.12625081829234078No Hit
CATGAAGTGAAATCAGTTTCAACACAACAGAAAACAAAGCTTAAAACATTG3230.12586115527292No Hit
TTCTTACATAAGATGAGTCTCCTTTAGTTAAACTGCTCATCATCCTGCTCA3220.12547149225349918No Hit
ACAGTAACATAAGCATTATCATCATTCGTACTTAAAGAACGGTCGCAATAG3200.12469216621465756No Hit
TGATCACACTGGATTTCATTCCATAGTTCTATTCGATTCCATTCGATGATG3180.12391284017581594No Hit
ACATAGGACATTACTGAAATGAGCATGTTACCTCGTATATCATCCTGGTTA3180.12391284017581594No Hit
ACATTATGCCTGGTAGTAGAAGTAACAGAACATTACTGTCTTATTTGCAAA3170.12352317715639514No Hit
GATAATACTTAACCACTTCACATAGTGTTTCAAGGTCAATTAATATCTGTT3170.12352317715639514No Hit
CAACCTTTAGTGATCATCTTAGCCACAAATCTTTCTTAGATGACTCCTCAA3110.12118519903987032No Hit
AACTTGTATCCATCATGATCACAAGAAATCCTAAATGGTTGATAATGTCAA3100.12079553602044951No Hit
CAGATTTTTCAAAAAGCGTGTTTCAAAATTGCTCAATGAAAAGAAAGGTTT3070.11962654696218711No Hit
ACACATAACAGTGAAACTTGCAAATCTCAGAGACAAAGAGAAAATCTTGAA3060.11923688394276628No Hit
TCATAGCAGAATGTATTGCACATGTGTTTGTGGTGATGCTAATGTAAACCT3060.11923688394276628No Hit
ATGAGAAAGTGTTCACATAATTGTACGCAATGATGAACACCCAGAATGTTG3040.11845755790392469No Hit
GTAGTATACGGTCTATTCAAAGCTGACAAAGAGCTTATAGTTAGTTAGGTG3030.1180678948845039No Hit
TGTATATAGACAGAAACATATGCATTCACATACACGTAATGATAATGCTGT3020.11767823186508307No Hit
CAGATAATCTACAGAATGGGAGAAAATTTTTGCAGACTATGCATTTGACAA3000.11689890582624148No Hit
ATGTAACAGACCAGGAATCTTTCAACAATGTAAAGCAATGGTTACAAGAAA2990.11650924280682066No Hit
GATGTGTATAACTAAAGTGTGGTTAGTAAAATGATTCTGCCACAAACAGAT2990.11650924280682066No Hit
TCCAAGTACATTGTCAACCCTGTAAATAGTGAAGCAAAGTTTATTCAAGAT2970.11572991676797904No Hit
GTTATTATTCATGCGACAAACAAGGTAGTCTTTAGATTCAACACGACACAA2970.11572991676797904No Hit
ATTTAAACTGATTCAAGGAAATGGACCAATTCATATTGTTGGAAACCACAT2960.11534025374855825No Hit
GTTAATTAAGTCACAAACTTAAGTGAAAAATAAGTTCCTGTCTCTTATACA2950.11495059072913745No Hit
CAGATATCAATGCCTTATGTGATGAATATTTAAGACTGGCGTATCATGGAT2930.11417126469029583No Hit
AGCTCTAGAATTACCACAGTTATCCAAGTAAACCATACGATCAAATGAACC2930.11417126469029583No Hit
AGTAGGATAAGCTGATGAGCAACTTAATGTCTACTTTCTATGCTAAGAATC2930.11417126469029583No Hit
GAATTGAATGGAATCATAGAATGGAATCCAGTGTAATCATCATCGAATTGA2900.11300227563203341No Hit
GTGTGATATTAAAATGCCCAAAGTAGATGGCATTGAGTTTCTTGAAAAGTC2860.1114436235543502No Hit
TTGGTGTGTATTCCTTCCACAAACTTACTGCTATCACCTGTATCTGTAGTT2860.1114436235543502No Hit
ATTTAAGTGGGTTGGTAGCTACTAATACAACATTGGATAGATCGAATCTAT2840.11066429751550859No Hit
GATGTATTACAAGAGTTGGAGCCTATTCATTTTGAGGTTGTTTTTGTTACG2840.11066429751550859No Hit
GATCAATGTATTATGGATAGGGATCGTGATTATGGTACTGGGTTTCATATC2830.11027463449608779No Hit
TATCGATTCTACGAAATACTAACCACTATACACTAACTACCTGTCTCTTAT2820.10988497147666697No Hit
GTTCTTCTTCAAAAAGTGCTATTCAGAATACGAACTAATTGGCTACATACA2820.10988497147666697No Hit
ATTTGTAACCTCGTTATCGTTTAATATTCGTAACCTAATTCCGTCAAGAAA2800.10910564543782537No Hit
ATGTATAAACAGAGCATTGAGAATTAAGCATTGTACAATTTTCAAGCTCGA2760.10754699336014216No Hit
ATTCTTCAGAGTAACTTTAAAATCAACCTGCCAACTTAAACCCTGTGTTGA2750.10715733034072134No Hit
ATAAACAGGAAATCTGAGCCTTCCACTTACTGAAATCTTGCTAAATAGGTT2750.10715733034072134No Hit
CTACAATACAAAGTGTTATGACGTCAAATACGTAATCTACCTGTCTCTTAT2740.10676766732130054No Hit
ATGTAATGCTGTATTCACATAATTGGACTTTGTTTTTGCATTGATGCTGTC2740.10676766732130054No Hit
ATCATAGCTACTGTTATTTACGCTTACTGCTCCTTTATACCAATATCTGTC2740.10676766732130054No Hit
GCGTAAGGTTTCATTTTCCTTTTTGAGTTCTTCATTTTGATGCTTAAGAAA2730.10637800430187974No Hit
CATATTTTGTGAATATCTTGCCTGTAAAGTTTGTTTCGTGATAGCTTGTAG2730.10637800430187974No Hit
GTATAGGAGCTATCGTAACTACAATATTGTCTGTTACAGGTTTACACGTTC2720.10598834128245893No Hit
TCATTGCTCTAATAATCTTCTGGTCCTACTATGCCTATGTTGTGGAGCTCT2720.10598834128245893No Hit
ATACTAATAACAGCAGCAAACGAAAGTTGAAAACCAATATCTTCTATCAAT2720.10598834128245893No Hit
GTATTCAAAAGACCTTCAAGAAGTAGAATCAGTAGGACATGATTACTGTTA2700.10520901524361731No Hit
GAAAAACACGTATCAAAATAGGTAAAAGTTGAAGGTAAATGCCTGTCTCTT2700.10520901524361731No Hit
GAATTAGTTTTAGTTTTGAAAGCTTTGAAATTCAAGAATGAGTGTTGTAAA2690.10481935222419651No Hit
ATACCATAGGAAGGGCCTGTATCCATTTTAATCCTGTCTCTTGACATACTT2690.10481935222419651No Hit
AAGACACATACATAGGAAAGTACAGAGGAATATAAGTGCTGTCTCTTATAC2680.10442968920477572No Hit
GACTATTTCAACTGTCCTTTTTGAAAATGGCAATTTGTTATCATGTAATCT2680.10442968920477572No Hit
GTACCCAACCATTTTTTTTCAACTTGTTTAGTATCTCTGTTACTTTGCCAT2680.10442968920477572No Hit
ACCTGATATGGTTTTTGTGTATGATGTTAAAGAACGAAAGTTTATTGTTCG2650.1032607001465133No Hit
GCATCCAAATTGGAAATGAAGAAATAAAACTCACTACCTAAATATGAGATG2650.1032607001465133No Hit
CTATTTCTTCATCACTTTTCTCATCAATAACTGTGTATGATGACTGTCTCT2630.10248137410767168No Hit
GAAAGTACTTGTGGTAATAATGGAACAGCAACTATAGTTGCAACAAGTAAT2610.10170204806883008No Hit
TTATTAGGCACAGTGAAGCATTAAGATTTGATTGCTTCTGTGTAAATAACT2590.10092272202998846No Hit
AGTCTCAGTCTTCTTAGAGAATACCTAAGTGGTTGTGAACAGTGTTAGTAG2580.10053305901056767No Hit
TTGAAAGCATGTTAACTGAAAGAACTCAGGCAAAAAAGACTACATATCTGT2580.10053305901056767No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGC453.8198777E-1045.00000437
AGCGTTA207.023034E-445.00000419
GTGCAGC406.7793735E-945.00000411
TTCTACG207.023034E-445.0000047
GGGTGTC700.045.00000442
GAGGACG207.023034E-445.00000417
CGTGGAA207.023034E-445.00000427
TGGAGCT351.2070086E-745.00000443
GATACGG351.2070086E-745.0000047
CTCACCA207.023034E-445.00000413
AGTCCCG207.023034E-445.00000420
ACTGCGA453.8198777E-1045.00000431
GCGACAA453.8198777E-1045.00000413
GGTGTGG207.023034E-445.00000438
CAAGTGA406.7793735E-945.0000049
ACGTTTC453.8198777E-1045.00000441
CCTAGGG207.023034E-445.00000440
TCACAGG207.023034E-445.00000432
GACACAC406.7793735E-945.00000441
CGTGAGT351.2070086E-745.00000435
Produced by FastQC (version 0.11.2)