FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_46.351000000430f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_46.351000000430f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230493
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC113864.939846329389613RNA PCR Primer, Index 46 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT15050.6529482457167897No Hit
TATTGTAACTTTGATCAAGGGCAAAGTTGAAGAAGTTGTTCTTCCTGTTGA5990.2598777403218319No Hit
GTGTTATACACATACTTGACAACATTGTGATAATATGCAAGTTCTAACAGT5210.22603723323484878No Hit
ATTCCATTCCATTCCATTCCATTCCATTCCATTACATTCCATTCCATTACA4370.18959361021809773No Hit
AAGTTACATGGACAGAAATAGAACTGCATTTTACTTCCTTTTGTCTTAATT4330.18785819959825242No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG4160.18048270446390996No Hit
TTCTTTTGTAATCTTCACAAGTTGTATCACATCTTCTTTGGCTTCTCCAAT3940.17093794605476087No Hit
AAATAGGTTTTGTAGCAACAAGAATACGTCGTTCAAAGTTCTTAAACAGTT3310.1436052287921976No Hit
TGTTTGTGGTTTCTATGTTGTACTATCGTTCATAGAAACTCTGGTATTTTT3300.14317137613723627No Hit
GATGTTACCGTATTGGACAGAGAGATGTTGTTAACGTTCATTACCTCATTG3240.14056826020746835No Hit
ATTCTGAACACAATAGGCATAAACTGTAAACATGATAAGGATAACAAACTA3190.13839899693266172No Hit
AAGTAACAGAAGGCTATTTGTAAGAAAGATAAAGGCTTGTGAACAAAGAAA3140.13622973365785512No Hit
GATATGGACTAGAATAGAATGGAATGGAATAGAATGGAAAGGAATGGTTTC3130.1357958810028938No Hit
GCTTTCAATTCGTTGTATTTACAACCTTGTGATCTCACATACACAGAATCA2950.12798653321359No Hit
ACGTATTTTCATTTAAATGCTCAGTGACATTTACTTACCTAACAGGTTCAT2900.1258172699387834No Hit
GTTCTTCCAATTCCACTTCAGGATTTGTTAGTGCATATTCTCTCCCTTTTG2890.12538341728382207No Hit
ACTTCCAATATTGTGTCATTCACGTTATAATTGCCTGGTTCAGCAACATAG2880.12494956462886075No Hit
GTATTACATGAGTTTGGTTCGCAAATAAACAGAACTCAATGTGTTTTGCGT2870.12451571197389943No Hit
GTTTATGCTACTGCCTTAGAGTTTTCATATACGCAAGCTCCATTGCAGATG2790.12104489073420885No Hit
TAGTATTATGCTCCAAGTATATATCCAAGCTGATCAATTCATAACACTTTC2790.12104489073420885No Hit
GATGTACATATCTCTTGCTATTGCATCAGTTGAAATATCATTTTTTCCCGG2790.12104489073420885No Hit
TCTATTATCTGAACTTTACATTGTCATGGACTGAAACTAACAGTGCAGATA2760.11974333276932488No Hit
AATCCTGATAGTCTTAAATATGGTTTGATCGTGTTCATGTTAGATTAACGC2750.11930948011436356No Hit
ATTCAAAGTGTAAGAACAGCAAACCTAACTAAACAAAAAGGGCTTTAGTTT2740.11887562745940225No Hit
TTGTATACCAGAAAGCAATGGATATTTGTTCATGTCTACATGCATATACAC2730.1184417748044409No Hit
GAATCAATCCGAATGTAATGGAATGGAATGGAATGGAATGGAATGGAATGG2720.1180079221494796No Hit
GATGTACTTCAACAGATGAATGGATAAAGAAGATGTGGCATATATGGCATA2690.11670636418459562No Hit
ATATAATCCTTGATGAGCTCTTGAATTAAAAAATCAAAGAAAGCTACACAG2620.11366939559986637No Hit
CTTTTATGTTGAATGGCCTTACTTTTATCAAGTCCATAAGCACATAAAACA2610.11323554294490505No Hit
GAACCAACTTAACATTACGTTTCTTCAAGGCGTAATTTACAACTTCCTCGT2560.11106627967009844No Hit
AATGTAGTTAATGTATGATGCATTAAACAACTCAGGTAAGAGCTTAGAAAA2540.1101985743601758No Hit
AGTAGGTATTAAGACCAGGATTTGTAAACAGAATTCCATTTAAGTAGCGAT2530.10976472170521447No Hit
ACCAATTACTAGTCCAACCACAATCATGGTGAAACTAAAGAGATTTAATTT2520.10933086905025316No Hit
ATCATGATCATTGAAGAACCGGAATTCTATCAAGTGATAATTTGTGTTTTG2480.10759545843040787No Hit
ATAAATGTCCTTATTTGTTGAGGAAAAAGGAAAGTAAGAGACCTAGTATTC2470.10716160577544653No Hit
ATTCATTGTTTGAGCAACAATATTTGCAACCTCCTCACAAGCAAATTCAAT2460.10672775312048521No Hit
TATCTGGAGGAATTCTATCCTACTCTTTCAAAACCCTTGCCATTCCTATTA2440.10586004781056259No Hit
CATTTTAACTTTACGTACCACCGAAATTCATCTCCAATTGTGGATATTTGT2440.10586004781056259No Hit
TTAGTAAGTTGATGATGTCTTTTATTAGTCATGAAATAGTCCTCCCAGTTC2420.10499234250063993No Hit
TCTTAAAACAGAACATCAACATACAAAACAATGCATTAAAAACCTGTCTCT2410.10455848984567861No Hit
ATCATGCAATTTTAAAAACCTTCCTTTCAACTTCTTTCAAAGCTACTGACT2390.10369078453575596No Hit
GAACAAATGATCTTCTTGTCTTCTGATGTCGCTTTAACTTTGGAGAAACTT2380.10325693188079466No Hit
TGACAAGTATGCACATGAGAAATATTCAAAGGAACGTAACACAAGTCGTTA2380.10325693188079466No Hit
CCAATAAACAAGGAACAGTTGATAAAGAGATAATTGGTTGAGCGATAAAAT2370.10282307922583331No Hit
GAATGGGATCATCATCAAATGGAATCGAATGGAATCATCTAATGAACTTGA2340.10152152126094935No Hit
ATTTAAAGGGACATCATTTATAGGGACAGACAACCCAGCTTATTCTCTTTT2330.10108766860598803No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTAT207.0076995E-445.0180171
GTAGGAC207.0076995E-445.0180171
CCAGGAG207.015199E-445.008254
TACGACC207.015199E-445.008254
AGTTACG207.015199E-445.008252
ATACGAC207.015199E-445.008253
AGTAACG207.015199E-445.008253
TAGAGTC253.876871E-545.0082474
CTAGAGC351.2045348E-745.0082473
GGACTAG502.1827873E-1145.0082476
CTAACCG253.876871E-545.0082474
AGCATGC351.2045348E-745.0082476
AGTCTAG253.876871E-545.0082475
GAGGCTC253.876871E-545.0082474
TCACGGA207.022705E-444.9984825
TCGCCTA207.022705E-444.9984840
CCTTCCT207.022705E-444.9984819
CTAGACC207.022705E-444.9984842
TGGAGTC253.881845E-544.998487
AGCCGTA253.881845E-544.9984830
Produced by FastQC (version 0.11.2)