FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_45.351000000430e6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_45.351000000430e6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138901
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC2106915.168357319241762RNA PCR Primer, Index 46 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT1465810.552839792370106No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG18151.3066860569758316No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC7200.518354799461487No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC5610.4038847812470753No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA2410.17350487037530327No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA2020.14542731873780607No Hit
AAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCA1840.1324684487512689No Hit
GAGTAAATGTTCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCATT1570.11303014377146313No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA1410.10151114822787453No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT502.1827873E-1144.992845
TCATTGC207.017394E-444.99279821
CTATCGC207.017394E-444.99279825
TGGATTG207.017394E-444.99279812
TCTATCG207.017394E-444.99279824
TGCCGTC23200.044.0231345
ATCCAGT250.002098686836.00720238
ATTTAAG250.00210240135.9942416
AATTTGA250.00210240135.9942429
GCTGCTA250.00210240135.9942430
AAGGTTA250.00210240135.9942431
ATCCCTA250.00210240135.9942429
ACTACGT250.00210240135.9942425
TATCGCT250.00210240135.9942426
ACAAGGT250.00210240135.994248
CAGCACT300.00511782430.0168114
AGATTGA300.00511782430.0168116
CATCTCG300.005126850730.00636
GGCTTTC300.005126850730.00636
CTTTTCG300.0051358929.99520143
Produced by FastQC (version 0.11.2)