FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_44.351000000430bf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_44.351000000430bf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218676
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC82713.7823080722164297RNA PCR Primer, Index 46 (95% over 21bp)
GTCTTCAATATTGTCAGATTTCTTGCGTTTTCCTGTATCTAAGCCTTGTAG12980.5935722255757376No Hit
ACAAACATAAGTCTCAGCCCTCAAAAATTTCCTGTTATGTCTTTGATTCTT8560.3914467065430134No Hit
ATTCCATGGTGTTTATTGCAAGATAAATCAGTGATATCATCGTAGACTCAG7410.3388574877901553No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT6770.3095904443103038No Hit
AATGAAAGGAGTCATCATCTAATGGAATCGCATGGAATCATCATCAAATGG5700.26065960599242716No Hit
TTTCTGTACAATTACTGTTACATTTACCCAGAATGGTAGCAGTACATACAA4870.22270390897949477No Hit
GTTTAGATTTAGATCTTTCTCACACTTTTATGCTTAAGACCATTTTAAGCA4750.21721633832702264No Hit
ATTAGTGTGTGAACTAGAACCCAAATAACCAAGTTGCTGAATTAACACATC4710.21538714810953194No Hit
GAGAGAAGAAGATGGAAGTGATTGAAGTGTAGATGATTGAACAGAGGAGAA4600.21035687501143244No Hit
ATTCCCACCTATGAGTGAGAATATGAGGTGTTTTGTTTTTTGTTCTTGCGA3900.1783460462053449No Hit
CAACATAACCATACAAACATTGCATGATCAAGAACTAAACATGATTTGTTT3800.1737730706616181No Hit
CATATTGATTTCTTTTCAACACTTTGGCTTTTTTTTAGGAAGAAACTTAAT3740.17102928533538203No Hit
ATCATCATCAATTGGAATCGAATGGAATCAACATCAAATGGAATCAAATGG3730.17057198778100935No Hit
AAGTACTACTATCTTCATGTACCCATCAAAATTGATCAATGGCCAATCATG3680.16828550000914594No Hit
ATGTTATACATTAGGACCATCACATGTTCTTCTCTTTAATCCCCAAACTTC3540.16188333424792845No Hit
TCTTATTGTTGTTTGTAGGTAGTGGTGATGTGATCGATGTATTAAAAGCAT3530.16142603669355576No Hit
TCATTCTGTTGATGTGATGTATCACATTGTTGATTTGCATATGTTTAACCA3520.16096873913918308No Hit
GTCATCATCTAATGGAATTGCATGGAATCATCATAAAATGGAATCGAATGG3250.14862170517112075No Hit
TTTTCTTGTGCCTGAATAAAGCTTTTGTAGTACTTCATTGAAAAGAAACAC3140.14359143207302127No Hit
TGGATTTAGTTTGCATCATGATTCTCTGTTTTAACCAATATCAAGCATTTT3100.14176224185553055No Hit
TTATATTTCCTTTACTCCTTTCTTCCTTGCTTATTGTCTTCTTTTGTAATT3050.13947575408366716No Hit
GTATAAACTCAACACCTGAGAAAGGAGAATACGTATCAAAGAACAATGTAT3030.1385611589749218No Hit
CATCAAATGTCAATACTTGTGTGTGTGTGTGAAAACGTTTCAAGTCATGTT2950.13490277853994037No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG2930.133988183431195No Hit
GGTTTTATTGAATCCAGTAACTGACAACCTGATATATTGTTTTCGCTTTTA2890.13215899321370428No Hit
GTTTTAATCCATTGTAATCCATAGGATTATGACTTGCAGTTACTTCAATAC2880.13170169565933162No Hit
AAATACCATTTTAACACTTCATGTGTACTCTAGGTCCCTCATATCTGAGTA2850.13032980299621358No Hit
GTTTACTCTTGTTGTTTTTGTCCTTTAAGAGGCATTGTTAGGTTGTTTATT2850.13032980299621358No Hit
ACTAAACTCAATGCCTAAAAGACAAATAATCCAGTTAAGAATTGGGCAGAA2820.12895791033309553No Hit
AAATAACCATGAATCTGGTAAACTACAAATGAGATTTGTTCATTTCCTGTC2700.12347033968062339No Hit
AATTCATAGTCTGTGAACATCATGCTTGAACTCTATTCTTTCTTTTAGATC2700.12347033968062339No Hit
TTCAGAATAGTGTTCAGTAGCAATGTTGAATGTGAGTATGCTTGTCTTGTA2600.11889736413689661No Hit
AGAAGAAGAAGAAAAGATACGAGCAAGAAGACTAAACACGAAAGCGATTTA2600.11889736413689661No Hit
TTATGATGATTCCATGCAATTCCATTAGATGATGACTCCTTTCATTTCCAT2590.1184400665825239No Hit
AGTATACTGTAACAGTTGTAAAAGATCAATATCCATCCATCCTAGTTGAAG2580.11798276902815123No Hit
GATGAATGTGGTTACGAAAATACAAAGAAAAAAAACATGAAGAGCAACAAA2560.11706817391940588No Hit
GAAGAATGTTTCTAAAAACTTTGGATTTGTGTGGGTTTCTCTATAATCTCG2560.11706817391940588No Hit
GTTTTTAGGTGTCACTTGGAATCAAAACTCTAGTTTGAGATATTTTTGTTG2560.11706817391940588No Hit
GTTCTACATCAAATATTTGAGCATCAGCTGGGAGAGAATTTTTTTCTTGAC2530.11569628125628784No Hit
CATCAGATTCTTCATCACTTTCTTTGACAACTTTTTTAGCCTTACTTTTAC2520.11523898370191517No Hit
AATGAGAGGTGATTCTGTTGTTGTTGATGATAATGTAGTTTCAAAACGTGG2500.11432438859316979No Hit
ATAATAAGCCTTGGTTTGATGTAATGGCTCGGGATGATTATTGTTATTCCT2490.11386709103879712No Hit
ACATTGTGAAGAAAAAGAAATCATCTTCCTCAAAGAGGAGGTGCGAGATGA2450.11203790082130641No Hit
ATATTGAGGGAAGCACAACAGAGGAAAGAAAGAGAAGAAGAAGAGAAAAAA2450.11203790082130641No Hit
ATTTGCAAAATGTTGGTAACAGGTAATCTAAATGACCAATAAGATGAGTGA2390.10929411549507034No Hit
GAAGTAATCTGATATTTGCTTCCATAACATTGTGTCATCTTCATTTGATAA2390.10929411549507034No Hit
TATTAGGTATTTAGAGGAAGCATTTCTTACAACAACACACAAAAGCAAAAG2380.10883681794069766No Hit
ATCACATTTATTGGTTGCACAAGATTGCTTAAAAACCCTAATTGGACAAGA2380.10883681794069766No Hit
AGATATTGTGCAGACTGAAGCTATTAAAATGTAAGCAACACAAATAGTAAA2350.10746492527757961No Hit
AGTTTTAGTTGGTCTTTTTTTTCAGGCATTTCTTCCTGTTTAACTTTATCG2330.10655033016883425No Hit
TATTCACACTTAAAGGATGCACATTCGATACTTTTCTCAGAAGATAGGACA2320.10609303261446157No Hit
TAATTGATGAGTTCATATATCAGTTCCAAGCCTTCACTCAATATCGATGCA2250.10289194973385284No Hit
TAGATAGGTAGGTAGATATGATGGGTTGACAGATGGACAGTTGAATACATA2250.10289194973385284No Hit
AACAAATACTTACCATTGTGTTAAAATTACCTACAACGTTCGATACAGTAA2230.10197735462510746No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACG253.8519524E-545.0561031
ATCAGCG302.1457345E-645.0354773
GTAGGTG302.1457345E-645.0354773
CACCTAT406.7375368E-945.025176
AACGTCC207.0251414E-444.99428631
CGGGTAC207.0251414E-444.99428617
GGCAGTT207.0251414E-444.9942867
ACCGGTA207.0251414E-444.99428629
CGAACCG207.0251414E-444.99428640
TTAATCG302.1593023E-644.99428615
CTCGTAC207.0251414E-444.99428625
GATCGCG207.0251414E-444.99428630
GCAACCA302.1593023E-644.99428621
TAGCGGG207.0251414E-444.99428643
GGTCCAT207.0251414E-444.9942868
CCGGAAA207.0251414E-444.99428628
GGATCGG207.0251414E-444.99428639
ACCTGAG302.1593023E-644.99428614
ATTCGAG207.0251414E-444.99428617
CTGACGA302.1593023E-644.99428643
Produced by FastQC (version 0.11.2)