FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_42.3510000004307c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_42.3510000004307c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197511
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC2049010.374105746009082RNA PCR Primer, Index 46 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT65963.339560834586428No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCG12420.6288257362881055No Hit
ATACTATGCAGTGTTTGTTACTTCCTATCTCAAATCCACAGTACATATGGT3220.16302889459321254No Hit
ATCTAATGGAATTGCATGGAATCATCATAAAATGGAATCGAATGGAATCAA2860.14480206165732543No Hit
TTTGAGTGTTTTTCTGTGTTTCAGTTTCCTTCCATTTAGCTCTGATTTTGA2760.13973905250846788No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC2500.12657522872143828No Hit
ACAAAGAACTAATCTTGCCAATGTTGTGTTGTTGTTGAAAAGTCTTGGAAT2460.1245500250618953No Hit
GTAATAGAGTGTGATGTTGTTAAAAACTATGCCTTTGTTCATATGCTGTCT2420.12252482140235228No Hit
ATTCACTATAGTGCAACAGTTATGACTTGCAAAGTTTGTGTATGAATGTGT2360.11948701591303774No Hit
CAGTGATTCCATTCAAGTCCATTTGATGATTGCTTTTCATTCCATTCAATG2260.11442400676418023No Hit
GACAGAAGCATTCTCAGAAACTTGTTTGTGATGTGTGTATTCAACTAACAG2250.11391770584929448No Hit
TACGTATTATACATGTACTGGCTCCAATAAGCTAGTACTGTCTCTTATACA2210.11189250218975146No Hit
CTGATTGAATCAATATGTCGAATCAAACAAATTCACAACAACATGTTGAAG2170.10986729853020845No Hit
CTTAAGAAATGTGCTGAAAACTACAATGAACTGGTTTCCCCATTACTTTCC2170.10986729853020845No Hit
AAGTTATCGATCATCATAACTTTGATGAAAGTGATAGAGGATCGCATACAA2140.10834839578555118No Hit
CAATATTCCTTTGCAGATCTAAACTAAAACAAAATGTTCCTCAAACATGAC2080.10531059029623667No Hit
AATCAAAACAGTTTTCCTTAGTGACCATGTATAATCCACGTATAATCCACA2040.10328538663669365No Hit
GTGTATAAGCATTGTTAAGGTTTCTAACATTTGCAGAATTTCCACATTTGC2030.10277908572180791No Hit
TGTTTTAACTAATCCCTTAACATCCAAAACAACCTGTCTCTTATACACATC2030.10277908572180791No Hit
TCATTACCTCTATTCTTACATTAGTTCCACCAATACTGTCTCTTATACACA2020.10227278480692213No Hit
TCCTGTTACTAAGATGTCTTAAGTGGAATAGAGTCATTCCAAGAACTACAA2000.10126018297715064No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC2000.10126018297715064No Hit
GAACCGAATGAAATCATCAACGAATGGAATCCAATGTAATCATCATAGAAT1980.10024758114737914No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAG302.1563483E-645.0000041
CCTGTTA302.1563483E-645.0000042
TTTCCCA302.1563483E-645.00000437
TATGCGC302.1563483E-645.00000436
ACGATGG302.1563483E-645.00000444
GCCTGCA302.1563483E-645.00000422
GGAACGA302.1563483E-645.00000423
CAGGAGG302.1563483E-645.00000419
GAACGAA302.1563483E-645.00000441
GATTGCG302.1563483E-645.00000418
AAGATTA302.1563483E-645.00000415
GGCTGTA302.1563483E-645.00000417
GCTGTAC302.1563483E-645.00000443
CCCGGAT302.1563483E-645.00000434
CTCTAGC302.1563483E-645.00000426
GGCCTCT302.1563483E-645.00000423
TGTCGCT302.1563483E-645.00000432
TCGAACG302.1563483E-645.00000439
TACGTCA302.1563483E-645.00000428
TGCAAGT302.1563483E-645.00000430
Produced by FastQC (version 0.11.2)