FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_40.35100000043046.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_40.35100000043046.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168796
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC51023.022583473542027No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT12510.7411313064290623No Hit
GAATGGAATGGAGTGAAATAAACTCGAGTGGAATGGAATGGAATGGAATAG4700.2784426171236285No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCG3390.20083414298917038No Hit
ATATACAACGACCACAGCAGTAAATGGAGATAGTTACTCTGTTGAAATGAC2740.1623261214720728No Hit
GAGTACAATTAAGTTCAGTGTTACATCAGAGCCTACGATAAAATCGGCAAC2200.13033484205786866No Hit
GCTTCAATCAATTGTTTCAGTTCTTCTTCTTTTGGCAAATACAGACGGTAT2040.12085594445366003No Hit
TTCTGGAATAGAATGCAGAACCTATAGTGCACAATTAAAGATTCCTGAAGG2010.11907865115287092No Hit
ACCTATGAGTGAGAGAATATGCGGTGTTTGGTTTTTTGTTCTTGTGATAGT1950.11552406455129269No Hit
ATGATGGGAGTATTTGCATAATGAAAAATGGCAAATGCCACAAATCAGGAA1840.10900732244839925No Hit
AATAAAGACCGCATCTACAAGATACCCATGCTTTAGTGCATAGTCTTTACA1740.10308301144576885No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGAGT253.8766244E-545.00000428
ACAGTCC207.01617E-445.00000427
GTCGAAA207.01617E-445.00000415
CATGCCT207.01617E-445.00000433
AGCCCTC253.8766244E-545.00000423
CCCTAGG253.8766244E-545.00000417
GGCACGA253.8766244E-545.00000412
CAACCGG207.01617E-445.00000440
GGTTTCG302.1544674E-645.00000425
GGGAGTG253.8766244E-545.00000413
CCTAACG207.01617E-445.00000416
ACCCTAG253.8766244E-545.00000416
CCATGTC207.01617E-445.00000424
CGTTCAC207.01617E-445.00000441
CGCCTTA207.01617E-445.00000435
CTAACGC207.01617E-445.00000431
ACAGGGG207.01617E-445.00000439
GTTCGGA207.01617E-445.00000420
AGGCACG253.8766244E-545.00000411
GCCCTCT253.8766244E-545.00000424
Produced by FastQC (version 0.11.2)