H23LNBCXX_l02n01_bir_1228_pcontrolb_4.35100000042bb7.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l02n01_bir_1228_pcontrolb_4.35100000042bb7.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	82149
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC	2771	3.3731390522100084	RNA PCR Primer, Index 27 (95% over 21bp)
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	241	0.2933693654213685	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGT	228	0.277544461892415	No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	178	0.21667944831951697	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	176	0.21424484777660108	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	171	0.20815834641931125	No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	169	0.20572374587639533	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	164	0.19963724451910553	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	148	0.18016044017577815	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	132	0.1606836358324508	No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	120	0.14607603257495527	No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	117	0.14242413176058139	No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	111	0.1351203301318336	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	111	0.1351203301318336	No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	103	0.12538192796016995	No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	92	0.11199162497413237	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCG	84	0.10225322280246868	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACG	20	6.9586805E-4	45.047558	2
GACGAAT	425	0.0	39.699432	26
ACGAATT	425	0.0	39.699432	27
CGTATGC	430	0.0	39.237816	41
CCCACGA	430	0.0	39.237816	19
CGAGACG	430	0.0	39.237816	23
GCCCACG	435	0.0	38.786804	18
CTATCTC	435	0.0	38.786804	35
TTGCTAT	435	0.0	38.786804	32
AATTGCT	435	0.0	38.786804	30
TCGTATG	435	0.0	38.786804	40
ATTGCTA	435	0.0	38.786804	31
ACGAGAC	435	0.0	38.786804	22
TGCCGTC	430	0.0	38.714645	45
CTCGTAT	440	0.0	38.346046	39
TATCTCG	440	0.0	38.346046	36
GTATGCC	440	0.0	38.346046	42
TGCTATC	440	0.0	38.346046	33
CGAATTG	450	0.0	37.993828	28
CACGAGA	445	0.0	37.91519	21