FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_39.3510000004301f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_39.3510000004301f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151876
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC44062.9010508572783062No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT7550.4971160683715663No Hit
GAATGGAATGGAATGGAATCAACTCGATTTCAATGGAATGAAATGGAATAG5210.34304300877031263No Hit
ATTCCAAAAGTTCAATTTCACAATCAAGATTTAGTACGTACTGAAAAGGAA5180.3410677131343991No Hit
GATACAAAAGAATAGGCATAAAGCGTCTTCTGTGAAAATCATGCATGTATA4930.3246069161684532No Hit
AGTCATTAAAACATCTGCTTTTTCTCCAGTTAGATCTATACAACTGGATGG4820.317364165503437No Hit
ATGTAAATGGATTGGAAGAAGGTAAATGGAAAAATTACTGGAGCAATGGTA4120.27127393399878846No Hit
AAGTGTTTCTGTAACAAATTTGAAAACGAAGAAAGGAACACAAACAGATTC4110.2706155021201506No Hit
CTATTGAGATAATCATGTGGTTTTTGTCATTAGTTCTGTTTATGTGATGAA4030.265348047091048No Hit
CTTCCTAATTCACACAAACTTAAACTCTAGTGTTATTTCCTGAACTTAAGA3990.2627143195764966No Hit
ATATGCATCACTATAACTAGTACTCCATCCGTCAATTTATAAGTTGTGCTT3830.2521794095182912No Hit
GTGTTAGTTAAGACACAATTTACGATAAACCGAAATACGTACACTCAGTTA3800.25020411388237773No Hit
TCACAATCTAAGTCTCAGTTCCTCTGTGAAACTTCTCTTGTTATACAATGA3640.23966920382417234No Hit
AAATATTCTACGTGCTAGATTTAACTGTTGTTTCCTTATGGCATCATTGAA3620.2383523400668967No Hit
ATATACAGCAATCAATGTAATGTACCACATTAACGAAGAAAAGGCAAAAAA3560.23440174879506967No Hit
TTACTATTCCATTGCAATCAACCATAGAAAGAATGAGCTTGAGCAGAAGAT3450.22715899813005347No Hit
AGTGGATACAACTACTTCTGTAAGATTATTGGTAGAAGGAGTTAAGCTTAA3400.22386683873686428No Hit
AGATATTGGGATATGATATTTGATCCTCAGAAGTCTTCTACTTTCGTAAAA3390.22320840685822646No Hit
CTTGTATGATCATTATTGGTTTTGGAGTAAGGAAAAAGAAAAGAAACGCAA3380.22254997497958862No Hit
GAATTATGTCAAGAGATTTCGATATCGAAACAATTTACGTAGACGGAATTT3380.22254997497958862No Hit
GGCATTTTTAATGTTCTTAATCGTTGTACTTGGAGATATTGTTGTTCGTAT3330.21925781558639942No Hit
TACTTGTACAGTACTTTCTTGGTTAAAGAAGTGAGACTTCATGTGATAATT3300.21728251995048595No Hit
GTCCTAATTCAATGATACGTGTAGCAATGGTATTGATGAATTGGTAATCAT3290.2166240880718481No Hit
CAATATAACTGTTGAAACAAAGCTAACATGTACATATCCAAGAAAAATTTC3290.2166240880718481No Hit
ATTGAATTAGTTTCATCGATTTGGTTTGGGTTTTGTTCATCGTACGGAATA3270.21530722431457242No Hit
TGATTGTATTGCCTTTCTGATCTACTACTGTTACATGAAGGTCTTCTTCAA3220.21201506492138322No Hit
AATCATCATCGAATGAAATCAAATGGAATGATCATATGGACTGGAATGGAA3220.21201506492138322No Hit
GGTACCTAGGAATAAACCTAACCAAAGAGGTAAAGGATCTATACCCTAAAC3210.2113566330427454No Hit
TTCTTACTCATTTGTGGAAGCAAAAAAGGTGATTTCCTAGAAGTATAAAGG3180.20938133740683187No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCG3110.20477231425636705No Hit
AAATGGAATCGAATGGAATCACCGAATAGAATCGAATGGAACAATCATCGA3080.20279701862045355No Hit
ATTAAATTGTTCTAACATCCATTCCATACGTTTGACACCTGGTTTAATACC3060.20148015486317786No Hit
TCGTATACGTGTCAAAATCATTGTTAATGGTATTGTACCCATCGATGATAT2960.1948958360767995No Hit
TACTCATAAAGATAACTAGCCCTTGCATTTCACTTGGAATAGCTTAAACAC2840.18699465353314546No Hit
TATCCCACCTTATACACCAGAAATGAACCCTATTGAACAAGTCTGGAAAGA2840.18699465353314546No Hit
GGTTGTAATAGCTTCATCATAAGCATTGGTATGCAAACTGTTACAGTTGTC2760.1817271985040428No Hit
ATGAAATGCCTTCATTTTTGCATTGAGTTTAACAACATTGGATTCACTTGC2740.1804103347467671No Hit
ATACATATCTGAGACAATCAGAAATTTGACGTGAACCTACAATTGATTTAC2700.17777660723221575No Hit
GAATTGAATCGTAGAATATCAAATCGAATTGAAAAGAATTGACTCAAATCG2690.17711817535357793No Hit
TGATAAAGAAGAAGGTATTGAAGTGATATTGTTGGAATGGGATGATTTTAT2690.17711817535357793No Hit
ATTATCAACTTGGTTGTTAGTGAAGGATTGTCAAAGTATTCCTCAAATGAC2670.17580131159630225No Hit
GTCTCATTAGGTAAAAATGAGATCTCAGTGAAATAGTCGCATTTTGCAAAC2670.17580131159630225No Hit
AAGGAATCACAATTATACCTTTGCAGATGCTACAAAAATACTGTTACCAAA2660.1751428797176644No Hit
GTTCTTTATCTGTGTTATCAGACTGTTCTTTATCTGTGTTATCAGACTGTT2630.1731675840817509No Hit
GTGTAACACTGATGTCGTTTTCAGTATCAAAATAATCCGTACATATGACAC2630.1731675840817509No Hit
ACTCCATATCAACCCTGAAATTAAAACGCTTGAAGATATTGAAACATGGGT2630.1731675840817509No Hit
AAGAGAAGTATAATGGTGTTAAATCACGAATCGAACAATTAAAAGCATAGG2630.1731675840817509No Hit
AACATACATCCTGCCTTTTCTGTATGAACTGTACCTTGGGTAACTAACTGA2620.17250915220311305No Hit
CATAAATACTCACTGAATTAACGGAAGAATCAAACATCAAATGAATGAAAT2540.16724169717401036No Hit
GAGTAGATGTATGAAACTAGTTTATACCTTTCAGAGATCAAGTACAGTGCT2530.16658326529537254No Hit
TTATTGTTCGCTAGAATTATCCGTTGTCCGTGAATCTGAATGAAGCATAAA2520.16592483341673472No Hit
AAACAACTCTAGAGCAAAGAAATTGGAGTCAATTCAATCAAATACGATGTT2520.16592483341673472No Hit
GATAACAACACATGAACAAAACAAGGAAAGGAGTTTTTTGTAAAAAAACAC2520.16592483341673472No Hit
CTTCTGGTTACCATATTGGGTTGTTTTCTTGTAGGCATACATCTTAAGGCA2500.16460796965945904No Hit
GATCAGGAATAGATGCTGTATTTTGTCAAATGCTTTCTCTGCATCTAATGA2490.1639495377808212No Hit
CTTTATTGCAGAAACTCTTACTCTTGTTCAGCTATTCCTTTTCTTTTCCAA2480.16329110590218335No Hit
TAACAAAGATTAATCAATGTAATCCACCACATCAATACACTAACTGTCTCT2480.16329110590218335No Hit
AGTATGTACTGAACCTTGTCTACATGGAAGATAAGATCAAGTTCACAAACA2440.16065737838763203No Hit
CCTGTATATTGCTCATACACCAATCAAATTGTAATCATCTCGTATTCCTAT2420.15934051463035634No Hit
AACATAATGTGTAGCTTCTCTATCTTTCTTGCTACATCGTGATATTGTAAG2400.15802365087308068No Hit
TATGAGAACAAATGACTTACACCTCTGATTAGTCCCAAATTTATCTTTGTT2390.15736521899444283No Hit
CTCTTTATCTTGCATGAAACTTAAGATAGATTCACTTGCTTCTTTAGCAGT2340.15407305960125364No Hit
GATTGAATTCGATTCTTTTCGATTCGATTCAATTAGATTCTTTTCGATTGG2340.15407305960125364No Hit
TCCAATTACTAGACTTTCGTGGGATAACTATCTAACAGTTTCTCCGAAAGA2290.15078090020806448No Hit
ATGATTCCATTCGAGTCCATTTGATGATTCCATTGGACTCCATTTGATGAT2290.15078090020806448No Hit
GGTGAAATAATGGCATTCTCATATGAAGACAAACTAAAAGAATTCAGCTCT2290.15078090020806448No Hit
TATCCAAAAGAAACAAGAGCAGTTTTTAACTCATTCTTATCAATGGTCCCA2280.15012246832942663No Hit
TGTTAAATGTCACTGGTTATTTTGTAATCCTAGGTAACTCATTATACCTGT2280.15012246832942663No Hit
AAATGAGGAAATTAAGGTTTAAGCATTTTGCCAAAAGAACAAAATACTATG2260.14880560457215097No Hit
TCATTAATGATTGTCATTTTCCTCACCTACAATTGAATGTATTTCGTTTAA2250.14814717269351313No Hit
TTCCAGTGTTGATGTTATTGCATTAGTTAGTTGTTTATGTGTCTCTCCCTT2240.14748874081487529No Hit
TAACAAATACCGTGTAAAATTATTCCTGCATATAACATCCAGCTACTGTTT2230.14683030893623744No Hit
ATCCATACCTGTTAAGGTTACGTTTGCAACGGTTGAATAATTGTTAACAAT2220.1461718770575996No Hit
CATATACAAGTGTATTGCATATGCATTCATATGAACTTAGTAGTTCCTGTC2190.14419658142168612No Hit
GTATTGAATGAAATTGTCATCAAACGGAATTCAAAGGAATCATCATCAAAT2180.14353814954304828No Hit
TAACAGGATTGTCTCCCTACTTCATAAAATTGTTTGCTTTTCCTCTTGTAA2170.14287971766441043No Hit
GTATTTATCAAATCTGTTCCTATCTAACGGAACGCTATTGGATCAAATGAC2160.1422212857857726No Hit
AAGATAAACTCAAAATGGATGAAAGATCTAAATGTGAGACAAGATTCCATC2140.14090442202849693No Hit
ATACAGATCATGCATACATTAGAAAGAACGCAACGTTTACCAATTACTATC2130.1402459901498591No Hit
TTAGGGAAAAGCATAGAGTCTTTTACGATTACAATACCCGACGGTACTAAA2130.1402459901498591No Hit
GATTCGAATGGAATCATCGAGTAAAATTGATTGAAATCATCATCAAATGGA2120.13958755827122127No Hit
ATCTTCATGTTGGGATGTATCAACATTTTCATCATCTTGTTGAATGTTGTT2110.13892912639258342No Hit
ATTCGATGATGATTCCATTCGATTCCATTCGATGATGATTGGATTCGATTC2100.13827069451394558No Hit
GTTTTGTTTCATGTTGCTTCTCTTGTACTTCTTAGCTTTCATGCAAAAAAA2090.13761226263530774No Hit
TAACAATACACTGAACTTATCATTTGCCATTAACCTAGATGCTAACCTGTT2080.13695383075666992No Hit
TAAAGAGACTGAAGTATCTTTTCCAGTCACAAGGGAATGCAACAAAAAATC2070.13629539887803208No Hit
GAATAAACACCAGTGGAATGAAATCGAATGGAATGAAATGGAATGGAATGG2060.13563696699939423No Hit
AACTTGGTCTATACTAACTTTGGCATAGACACACTATAACACAAGCATAGA2040.13432010324211857No Hit
AGATTTAGCTCTGAAATCAGTATGGTGTTTAGATACAAGCTCTTAATTCTG2040.13432010324211857No Hit
GAAATAAGTTTAATACCTTCTTCAGGAGTGATAGTTACAACAGCTTCTGAC2040.13432010324211857No Hit
TTTCATAATTGCTTGTCTCTGATGTTAGAGGAAAAACTTTCAGAGGAAAAG2010.13234480760620507No Hit
TGGGATAATATTCAGCCATAATAAAGGGAAAATGTCTTTTGCAGCAACATG2010.13234480760620507No Hit
CATCAAATCCAAGCTTCTTAGCAAATATGAGCATATCCTTCATCAATTCTT2000.13168637572756722No Hit
ATTCATACCAGAGAGAAAAAAGTTATGAGAAAAGATGAAAGGATCATTGGT1990.13102794384892938No Hit
GATCATACTATGCGTATTTTCTGACCACAACACTATATTTGAAGTCAACCA1970.12971108009165372No Hit
GTTTCTGGGTCATATTACCATATGTTAACTCTAGGTTTAACATTTTGAGCA1960.12905264821301587No Hit
TTTCTAATCATTCTGCTAGGCATATTTTACCAAGACATGTAGATTGATGAT1930.12707735257710237No Hit
TATCATTGCACTCTATACCATGATTTAGTGGTCCATTTTGACACTTTTTAA1910.12576048881982668No Hit
AAATAATTCTGCTCCATTAAAATCAACGATATATTGAACTGACCCACCTGT1900.12510205694118887No Hit
ATGAATTGATGGGGTATAAACTGTATTTTGGTGCCTAAAGAAATAAAAGCA1880.1237851931839132No Hit
AATTTGAACGAGCTATACAAATTGTTAGAAACGACAAAATAAAATCACAAT1880.1237851931839132No Hit
ATATGTAATAACACCCTCATTACATGATTTCCTCTAGGTTGAAGTGACTCT1870.12312676130527536No Hit
CTATTCTTGTTTCATGAATGCAGTATTTTCTCTCGTCCTTCTAATAAACTA1870.12312676130527536No Hit
ATATAAAGCTTGATATCCATAGAATGTTTCAAGAAGATTGCCTCTTGATAT1860.12246832942663752No Hit
ATAGATAGGATTGGTAGGGTTAAACTGTAAAGCAGCACCTAACAAACTACC1860.12246832942663752No Hit
GTCAAGAACTGAACACATTGTATTTGCGTTTCTTATAGTCACTTTCTTAGG1850.12180989754799969No Hit
ATTCAATTCCATTCGAAGATGATTCCATTCAATTCCATTCGATGATGATTC1850.12180989754799969No Hit
GTTCCGAAATAGTCCATTATGCCTTATCAAAATAACTTCTAACTTTGTGTA1850.12180989754799969No Hit
ATAAAACACAATGTCGAGTGAGGGAAATTAACCATCATGGTACACTTAAAA1840.12115146566936184No Hit
GTCATCATCTAATGGAATCGCATGGAATCATCATCAAATGGAATCGAATGG1830.120493033790724No Hit
AGGTTATTCCGATCAGGTGTTAAATGAAGTGAACTTGATAATAGGTAGTAA1830.120493033790724No Hit
TCATAGTTGAAAACGATGTTCACTCTTTCAATATCCATACCTCGCCCAAAT1800.11851773815481051No Hit
GATTATTGTTAATGTGTACCTCTGCTGTGTTTGTAATATCTTCTGTCGGAG1790.11785930627617267No Hit
GTGACATGCAATGCTAAATGAAAAAACGGATTGTGATCGAGTTGAAATTTT1790.11785930627617267No Hit
GTATACGGATTCAAATACATCCAACTAAACATTTGAAAATTGAGCCATGGA1790.11785930627617267No Hit
GACATACAAGTATATGCTTCTGATATTGATTCAGAAATGATTGAAATTGCA1780.11720087439753483No Hit
TATATCCTCAGGGAGATGACTAATTAACCACAAAAGGACCAAAGGAAAACA1780.11720087439753483No Hit
TTGTTTTTGACAAGAAATCTATTCCAATAGTTGCCTGATAAGTGTTATCGA1780.11720087439753483No Hit
ATGAATGAGTTGAAAAAATGAAAACATCTTAAGAGTCCATTAACTGTCTCT1780.11720087439753483No Hit
TTGCTAATTCTTTAAGAGTATCCTTTGTACCTTTTCTTCTTTTCCATTCCT1770.11654244251889699No Hit
GATAGATAAAATTGTAAACGAACATAGAGGGGGCTATTGTTATGAACAAAA1770.11654244251889699No Hit
GAAAAGAATCGAATCGAATCGTATTGAATCGAATCGAGTAGAAAAGAGTTG1750.11522557876162132No Hit
ACCATATGTGTTTTATTGATGAGTTTGTGAACGATAAAAATCAGCACCATG1740.11456714688298347No Hit
ACATGAATCTTCAAGAAATTCAAGAGCTAATAGACACCACAACAGAAGAAT1730.11390871500434566No Hit
GTAGTAACTCATGTTTAGCTCCCCTTCCATTCTAAGGTTTGGAATTTAGCT1710.11259185124706998No Hit
TTATTATGTTATGTTCCTTGTATACCCAGTCTGTTAAGAGTTTTCATCCTG1710.11259185124706998No Hit
AACTACAACTCACATTATACTTACTGTTGAGAAATTGGAGAGTTTTTCACT1700.11193341936843214No Hit
AGTTACATGTTGAAATGGTAATATTTTGGGTGTAGTAGGTTAATAGCTGTC1700.11193341936843214No Hit
AAAGTAAGTAGCGGATGAATGATTAATTCCATTCACATTTACTCGGTGTTA1690.11127498748979431No Hit
ATGTAGTTCTACTTGTTTATTTTTGCTTTGTTTTTGCTTTTGGTTTCAAAT1680.11061655561115646No Hit
AGCATCTTATTTGCGAAGATATTGCAACCCAAAAGTTGTTTCATGTTTCAC1680.11061655561115646No Hit
CTTAATGTACTGTTGTATCCTATTGGCTAGTATATTGTTGAGAAATTTTGC1670.10995812373251863No Hit
GACTTAACAATATTCCAGTTTGAGAACGGATAGAGTTGAGTGTATAATTAC1670.10995812373251863No Hit
ATATACAACAGCTTGGAAAACAACATTTAGAAAACAAAAGCCAATGTAAAA1670.10995812373251863No Hit
AGTATAATCTATTGCCAACTTGTTTAAGTTCAGGAGTTATCACATCAGCTA1660.10929969185388079No Hit
AACATAATCAGGTTATCGCCAATTACTTCTGTCATGAATTTTTTTACTCCT1660.10929969185388079No Hit
CAATTGATCAATCTAAAACAAATCAAAACAACCAACATTCTGTAGCTGAAA1660.10929969185388079No Hit
ATGTACATGGATTATTCTTCTTGCATATCAAGACAACAAAAGCACTACAAT1640.10798282809660513No Hit
GATTTAGATAAGCTTTTAGAGCTTAAAGATGTTGCATTTGTTGAAAAAGAC1640.10798282809660513No Hit
GAGTAGAGTAGACTACTACTCTACTCTAGTAGTAGAGTAGTACTACTACTA1630.1073243962179673No Hit
GTGTCAATTCAGTGATTTAATTACCACGGCTTTACTTTCATTTCCTTTCAT1630.1073243962179673No Hit
CAATAAGGGAAGATAAGAAGTACTCTTTTTTTCTCAAGGCACTTACTTTAG1620.10666596433932946No Hit
CAATAAAGGATACTGAAGATAAAGATTGTGCCACTAACAAAATCCAATATC1620.10666596433932946No Hit
GTAAGCTTATAGTTGAACTCAATATTAACCATCAATCAAGTGGATCTAATT1620.10666596433932946No Hit
AACAGGAATAGAGCAATTAGGATTTGACTTTTAACTAGGACAAAGGTGGAA1610.10600753246069161No Hit
ATCTCACATAGTTACCTTTCTTTCTTTTTTTTGGTGAGAGTACCTAAAATC1610.10600753246069161No Hit
ATTTACAATAGCAAACCAAGTGTCCATCAATAGATGAATGAATACAGAAGA1610.10600753246069161No Hit
TGTTGGAACAATGATAGAATTCTGGAGTTTTCGAATTACCAATTAAACTTA1610.10600753246069161No Hit
ATATTCCCACAGCTCCTTTTGTTTTAACAGACAAGAAAGAGGATATAGCCA1570.10337380494614028No Hit
ATTATATGGACCTAAAGCTGCCTTCAACTTTGGTCACTTTGTAGAACCTAA1570.10337380494614028No Hit
TCTAACTCGTCATTAGTCAGATTAGTCAGAAGATAATGAAACAATGTGTTT1560.10271537306750245No Hit
TTTCATTTGTTGTCATACAGATAATCACTGGGTTTAGAAATCAGGTATCAC1560.10271537306750245No Hit
CAGTAAAAGTTGGTAGAATGAGTTGTGTAATTGACTAATCCTACACAGTAA1560.10271537306750245No Hit
ATATATCGAAGTGATGATTTGTTGGCGTAATAACATCAATAGCGTCACAGG1540.10139850931022677No Hit
AAACTCTACAACAGCTTTGAGGTCATAACTTACATAAAGCTGAAAAGGTTG1540.10139850931022677No Hit
GAGTTTAATGAAGCGAGAGGAAGTCGTGATAGAAGATAATCGTTCATATCT1540.10139850931022677No Hit
ACTTACTATTTAACCAGTCGATATGTTATCGCCTCAGAACTGTAATTATGT1540.10139850931022677No Hit
GATCTTATTTATGCCTAGAAAATCCTAAAGACTCCTACAAAAGACTCCTAA1540.10139850931022677No Hit
GAACTAAACCTTTTGCTAAGATTTGTTTACCAACTTCAAATTGTTGGTCAA1540.10139850931022677No Hit
CATCATGCTCTTGTATCATTTCTTGCATCATTGGAATATTTTTGAAGATGG1540.10139850931022677No Hit
CTCTAAATCAATGTATTCAGATATGACACTGACGATATCTGTCTTTTCACG1540.10139850931022677No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAG207.0139393E-445.00000411
GAGGATT207.0139393E-445.00000429
CAGGTCT207.0139393E-445.0000041
GTGCAAA207.0139393E-445.00000413
ACTATTC207.0139393E-445.0000043
CTATGGC207.0139393E-445.00000441
TAGAGCC207.0139393E-445.00000437
CTCACTG453.8016879E-1045.0000049
TCGCATT453.8016879E-1045.00000437
GGCAGAG253.8747774E-545.00000433
CCCCTGT207.0139393E-445.00000416
CCCCTGG253.8747774E-545.00000440
GCCTTAT253.8747774E-545.00000421
GGTACCT207.0139393E-445.0000041
GTCGCAT453.8016879E-1045.00000436
AGATCTC453.8016879E-1045.00000420
ACACGTT253.8747774E-545.00000439
GCCCAAA207.0139393E-445.00000444
GGTGTAG253.8747774E-545.00000429
CGTATAC453.8016879E-1045.0000042
Produced by FastQC (version 0.11.2)