FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_38.35100000043002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_38.35100000043002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239500
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC131935.508559498956159No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT21770.9089770354906055No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCG6450.26931106471816285No Hit
GAAGCATTTGTTGTACTTGAGACTTCAATTCACGTGGAGAATCAACTTCAC3480.1453027139874739No Hit
AATCAATATCACGTCACAGGTGTTGTTTGATTACATTTTACTGATCCCATC3000.12526096033402923No Hit
AATTTGAACTCCACAAAAACAATGAACTGAAAAGAATGTCTTTATTGCTCA2760.11524008350730688No Hit
TAATGTTGTCTGTTCACATTTCAAAAATGCAAACTCATTTCATTCTTCAGA2750.11482254697286012No Hit
TAACATTTGTTCCCGCACAAATTGTAGTACTTGTAGAAGTAATTGAAACAC2750.11482254697286012No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC2630.10981210855949895No Hit
CTGAAGATATGCTAAACATAACAACTCAATAGGTACTAATTTTGGCATCAG2630.10981210855949895No Hit
AACACAAAGAGTTTTGAAAACATTTCGAAGTGGTTAAGAAACATTGAAGAG2630.10981210855949895No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGT206.9950015E-445.0353131
CCAGGGT302.1528413E-645.01653
TGTTACG207.009419E-445.01652
ATTCGGT207.0166367E-445.00716
TAAGCAG207.0166367E-445.00715
AGAGCCG253.8779137E-545.00715
TCGCCAG302.1590422E-644.99770438
GTCGCTG207.0238597E-444.99770438
CGAGTTT253.8827002E-544.99770418
AATCACG207.0238597E-444.99770441
GGTCGCT207.0238597E-444.99770437
AGCCCTG207.0238597E-444.99770424
TAGGTCG207.0238597E-444.99770435
TGGGTGA302.1590422E-644.99770414
CGTGAAT207.0238597E-444.99770424
AGGACTT207.0238597E-444.9977048
ACCGACA253.8827002E-544.99770437
TGCGGAG302.1590422E-644.99770430
ATGTACG253.8827002E-544.99770435
CAGGGTT253.8827002E-544.99770436
Produced by FastQC (version 0.11.2)