FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_37.35100000042fea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_37.35100000042fea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151270
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC147509.75077675679249No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT114297.555364579890263No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCG17311.1443114960005287No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC6180.40854101936933956No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC4780.3159912738811397No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA2600.17187809876379984No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA2030.13419713095788988No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA1680.11105969458583989No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC14250.044.68388445
GTATTGA250.002098007436.011641
GATGTGG250.00210141735.99973325
CTCGGGA250.00210141735.99973333
AAGCCTC352.809008E-432.1426227
TATTAGT352.809008E-432.142622
CTGTGTC352.809008E-432.142629
ATGTAGC300.00513348729.99977722
AAACGTT300.00513348729.99977713
GGTATAC300.00513348729.99977727
CTCTGCC300.00513348729.99977741
CGATAAA300.00513348729.99977742
AGTGTTG300.00513348729.99977723
AATCCTC406.14148E-428.12479435
ACTGTGT709.218875E-725.7140948
GTTTAAG450.001219333325.0080831
GCTTTGA450.001219333325.0080831
ATGCCGT25700.024.77608344
ATATCTA551.3606143E-424.54527312
TATCTAT551.3606143E-424.54527313
Produced by FastQC (version 0.11.2)