FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_36.35100000042fc0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_36.35100000042fc0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271407
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC47841.7626664013824258No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT5770.2125958431433235No Hit
ACAAACATAAGTCTCAGCCCTCAAAAATTTCCTGTTATGTCTTTGATTCTT5710.21038514113490073No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAA5110.18827812105067296No Hit
GAGTAGTAGTTACCATCAAACAAACTATAACTGATTTAATGAGCCATTCGC3910.14406408088221748No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG207.026306E-444.99668529
CTGTATA207.026306E-444.9966857
TTTCGCG207.026306E-444.99668540
ACCTGGC351.2080454E-744.99668534
TCAGCCC800.044.99668514
GCTAGGT207.026306E-444.99668545
TGCCGTC5200.042.8333845
AGCCCTC900.039.9970516
CAGCCCT900.039.9970515
ATGCCGT5900.037.75145344
CCTAGTA301.13441994E-437.5179752
CCCTAGC301.1381191E-437.4972414
CATTATA250.002095538436.0305371
TTAACGG250.002099335436.0172544
CCCTCAT250.002105041235.9973538
CTATCGT250.002105041235.9973538
ACGCACA250.002105041235.9973524
CGCATAA250.002105041235.9973528
GGATCAC250.002105041235.9973534
CCTACGG250.002105041235.9973534
Produced by FastQC (version 0.11.2)