FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_35.35100000042f9a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_35.35100000042f9a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323532
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC113863.519280936661598No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT18300.565631838581655No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCG5130.15856236786469344No Hit
AGTCTATATAGTCCACATTGATGACCACTATTGTTGGACCAAGTCCTCTCC3930.12147175549868329No Hit
GTATAATCCCTGATGAATAAGTGTTCTACTATGTTTCCGTTCCTGTGTTAT3420.10570824524312897No Hit
GGTTTATTATTCCCCCTGAAAATCAGAGCACTTAACAGAATTACACAAATC3380.1044718914975953No Hit
ACAAACATAAGTCTCAGCCCTCAAAAATTTCCTGTTATGTCTTTGATTCTT3300.10199918400652795No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGC253.872368E-545.0239754
GTGCACA302.1619253E-644.99613611
TAACGCG253.8865408E-544.99613615
CCACTAG302.1619253E-644.99613639
AACGCGG253.8865408E-544.99613616
CGAGCAA302.1619253E-644.99613637
AGCCTAG253.8865408E-544.99613635
TCGAGCA302.1619253E-644.99613636
TTCGCAA302.1619253E-644.99613636
GACTAAG302.1619253E-644.99613619
AGCGTAC207.0287375E-444.9961343
GCGACTA207.0287375E-444.9961313
TTGTGCG207.0287375E-444.9961329
GCGGTGT207.0287375E-444.9961333
GCGAAAT207.0287375E-444.9961344
GGACTCG207.0287375E-444.9961318
TGTGCGT207.0287375E-444.9961330
AGCGGTG207.0287375E-444.9961332
GAGCGAC207.0287375E-444.9961311
AGCGACT207.0287375E-444.9961312
Produced by FastQC (version 0.11.2)