FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_31.35100000042f20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_31.35100000042f20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC35.263157894736842RNA PCR Primer, Index 46 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC23.508771929824561No Hit
ATAGTACAACTTTAATTCTGCTTGTTGATGGTGTTAATGTGTATGAGAGCC11.7543859649122806No Hit
GTTACTAACTCTCAATCAAGTCATTTTCCTTCTCAACAATTAGTAGATCAA11.7543859649122806No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCTTAAAAACACATCC11.7543859649122806No Hit
CTCTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGCCACCTCC11.7543859649122806RNA PCR Primer, Index 23 (96% over 28bp)
GTAGGAGTATATCTCTATTTGCAGATGACATGATCCCTGTCTCTTATACAC11.7543859649122806No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACCTGTCTC11.7543859649122806No Hit
CTTATACACATCGAGCCCACGAGACGTTAGGTCATCTCGTATGCCGTCTTC11.7543859649122806Illumina PCR Primer Index 7 (95% over 23bp)
CTCTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATGCGATAA11.7543859649122806RNA PCR Primer, Index 23 (96% over 28bp)
GTACTCTGCGTTGATACCCCCGTACTCTGCGTTGATACTGTCTCTTATACA11.7543859649122806No Hit
CTTATACACATCTCCGAGCCCACGATACCTTGCAGAATCTCGTATGCCGTC11.7543859649122806No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAGCTGAATCTCGTATGCCGTC11.7543859649122806No Hit
CTCTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAACTAGG11.7543859649122806RNA PCR Primer, Index 23 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCTTTCAGAATCTCGTATGCCGTC11.7543859649122806RNA PCR Primer, Index 40 (95% over 21bp)
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACAT11.7543859649122806No Hit
GGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGCGAGAGG11.7543859649122806Illumina PCR Primer Index 11 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACCTTGCTGGATCTCGTATGCCGTC11.7543859649122806No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGTC11.7543859649122806No Hit
TATCAACGCAGAGTACTTTTTTTTTTTCTGTCTCTTATACACATCTCCGAG11.7543859649122806No Hit
ATCAACGCAGAGTACGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA11.7543859649122806No Hit
CTTATACACACTCCGAGCCCACGACTCCTGGTCATCTCGTATGCCGTCTTC11.7543859649122806TruSeq Adapter, Index 7 (95% over 22bp)
GCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAA11.7543859649122806Illumina PCR Primer Index 11 (96% over 29bp)
TATTATAATAGCGTACGTTTTTTGTCATACTAACCCAACAATAGGTTTGTA11.7543859649122806No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA11.7543859649122806No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCTCTGGATCCCGTATGCCGTC11.7543859649122806No Hit
CTCCTATGGGAGTGAAGTGGAAACAATCAAGCTTCCAAGAAAAGCTCTAAA11.7543859649122806No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCGTC11.7543859649122806RNA PCR Primer, Index 45 (95% over 21bp)
CCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA11.7543859649122806No Hit
TATATATTGAGGTAGAGTAAATTCCAAAGGTAGATAAGCACTACCAGTAAT11.7543859649122806No Hit
AAAGTACTCTGCGTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACA11.7543859649122806No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGATTCTCGTATGCCGTC11.7543859649122806No Hit
GTCCTTTGGATGTGTTCATGAAAAAACAAGGATTCTCTTACTCAGACAGCT11.7543859649122806No Hit
CCATTTCTACTTTATCCTCAATTTGCATCTCTTTATCTTTGAACAACTGCA11.7543859649122806No Hit
CTTTCGGAATCTCGGTTGATTTCTGTTCCTGCAGCTACTTAGATGTTTCAG11.7543859649122806No Hit
GTATCAACGCAGAGTACTTTTTTTTTCTGTCTCTTATACACATCTCCGAGC11.7543859649122806No Hit
GCATGTGTATATTTGGTGTCAATAGGATGCTTTCTTAATGTTTTAAAGAAG11.7543859649122806No Hit
GGGTATCAACGCAGAGTACTTTTTCTTTTTTTTAAAAAAAAAAAAAAAAAA11.7543859649122806No Hit
CTCTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGAAAAGAA11.7543859649122806RNA PCR Primer, Index 23 (96% over 28bp)
CTTATACACATCTCCGCCCACGAGACGGCATAGGATCTCGTATGCCGTCTT11.7543859649122806RNA PCR Primer, Index 24 (95% over 21bp)
GACTCGAATGGAATCATCATAGAATGGAATCGAATGCAATTATCGAATGGA11.7543859649122806No Hit
GCAGAGAATTCTCTTATCCAACATCAACATCTTGGTCAGATTTGAACTCTT11.7543859649122806No Hit
GTACTTTTTTTTTTTTTTTTTTGCGTTGATACCCCTCCCGTACTCCTGTCT11.7543859649122806No Hit
AGAGACAGCTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTC11.7543859649122806No Hit
TCCAAGAAGAAATTCAAAAGAAGATGAATGTATGAAACAGACTCTGTCTCT11.7543859649122806No Hit
GTGTACGCATCTACACATTACACTCAAGTGACACTACTTTAGTATCCTGCA11.7543859649122806No Hit
GGTATCAACGCAGAGTACGGGGCAGTGGCTGTCTCTTATACACATCTCCGA11.7543859649122806No Hit
GCACCAGGAACCACGCCACCACCAAAAGCCACCAACGACCAACCACTACCC11.7543859649122806No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC11.7543859649122806No Hit
GTATCGGGTTTTCTTTTGAGGGAATAAATCCTGCTCGAGTTCTCTTTTTGA11.7543859649122806No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGGCAGAATCTCGTATGCCGTC11.7543859649122806No Hit
CATCATGGGATATGTCTGTTCGTTTGTACGATGTAACAGAAAACAGCCAAC11.7543859649122806No Hit
GTACTTCTTTTTTTTTTTTTTTTTTTCCCCGTACCTTTCTTTAAACCTTTT11.7543859649122806No Hit
GGATTCATCTCAGCGACCGTGAGGCCAACCTGACTATGGCGCAACTGGGCC11.7543859649122806No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC50.045.017
GTATGCC50.045.042
TGGCAGA50.045.030
AATCTCG50.045.036
ATCTCGT50.045.037
ATCTCCG50.045.010
CACATCT50.045.07
GCCCACG50.045.018
TATACAC50.045.03
TGCCGTC50.045.045
CCACGAG50.045.020
TATGCCG50.045.043
CATCTCC50.045.09
GAGCCCA50.045.016
CACGAGA50.045.021
CCCACGA50.045.019
ACATCTC50.045.08
TTATACA50.045.02
TACACAT50.045.05
ACACATC50.045.06
Produced by FastQC (version 0.11.2)