FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_30.35100000042ef8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_30.35100000042ef8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59
Sequences flagged as poor quality0
Sequence length51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC35.084745762711865No Hit
GGTATCAACGCAGAGTACGGGCAGTGGCTGTCTCTTATACACATCTCCGAG11.694915254237288No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC11.694915254237288No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTTCCGAATCTCGTATGCCGTC11.694915254237288RNA PCR Primer, Index 46 (95% over 21bp)
GGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAACCTCC11.694915254237288Illumina PCR Primer Index 11 (96% over 30bp)
TATCAACGCAAGAGTACGGGGGGGCGGGGGAAAAAAAAAAAAAAAAAAAAA11.694915254237288No Hit
CAGTTATAGTTTGTTTGATGGTAACTACTACTCGGATAACCGTAGTAATTC11.694915254237288No Hit
GATCTACTTATTAATCAGAATAACCGTATTGCTCATCCTTTGGCAACCCTT11.694915254237288No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCTGTCTCTTATACACATCTCC11.694915254237288No Hit
CAATTACATCAGCTAAAGTTTATGATGCTTACTATCTGTCTCTTATACACA11.694915254237288No Hit
GAGTACGGGACATACTTCTTTATGTACCCATATGAACATACAATGCTGTCT11.694915254237288No Hit
ATAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACTGCTCCT11.694915254237288RNA PCR Primer, Index 33 (96% over 28bp)
ATAGGAGATAGAACAACCAACACATTCTGTGGAACACCTGATTATATTGCA11.694915254237288No Hit
ATACTGAATGAATAAAGACTTTTGCTTGAACCTGTCTCTTATACACATCTC11.694915254237288No Hit
CCTAAGATCTGTGCATATCACTGTGTTTAAATTAAACCTGTCTCTTATACA11.694915254237288No Hit
GGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCCCTTATG11.694915254237288No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTT11.694915254237288No Hit
CTACAGGATAAGGAATGCTGAATGTTCCATCAATATTACTGATGAATTCGT11.694915254237288No Hit
CTTATACACATCTCCGAGCCCACGAGACCCCTGGGCATCTCGTATGCCGTC11.694915254237288No Hit
CTAGTAAACAATGCTGTAAGAATCTAGAAATTAAGGGCTTCAATACCTGTC11.694915254237288No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT11.694915254237288No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTCCTTCATCTCGTATGCCG11.694915254237288No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGTC11.694915254237288No Hit
GACATGGTTATCTATTCTGGAATTGGTTTGGTGAATTACTGTTTTGTCTGG11.694915254237288No Hit
GCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA11.694915254237288No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAACAGAATCTCGTATGCCGTC11.694915254237288No Hit
CTTATACACATCTCCGAGCCCATGAGACCATTCCGAATCTCGTATGCCGTC11.694915254237288No Hit
GTATCAACGCAGAGTACGGGTGGTTTGACCGGTTGTCCATTAACTACATCT11.694915254237288No Hit
ATGTCTCATCGACATGCCATTTATAATAAGCTTTTTTATGCTTTTGCTTCC11.694915254237288No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA11.694915254237288No Hit
GAAAGAGAGAGTAGCGCGAGCACAGCTAAGGCCACGGAGCGAGACATCTCG11.694915254237288No Hit
TACTATAAGTACCTCATGTGAGTGGAATCATACCTGTCTCTTATACACATC11.694915254237288No Hit
CTCTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAAATGACTAA11.694915254237288RNA PCR Primer, Index 23 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCTTGCTGAATCTCGTATGCCGTC11.694915254237288No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.694915254237288No Hit
CGCTTAACTATCTTAACAAGCTTTGAGTGCAAGAGATTGAAGAGTTCAAAT11.694915254237288No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATCC11.694915254237288No Hit
ATTACACAGATGCACCAGTTTGCTCATACATTCACCTATTGAGGGATGTCT11.694915254237288No Hit
GAGTACGGGGGGGCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG11.694915254237288No Hit
TTGCTTTACCAAAAGGAAAGTATGTCACTTATGTTGTGATAGACCAAAGAT11.694915254237288No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAGTACCTGTCT11.694915254237288No Hit
ATCCATGTCCAAAACTTTAAAAACGCAAAGACCACACAAAAAGATAGTCAT11.694915254237288No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTCCGCATCTCGTATGCCGTC11.694915254237288TruSeq Adapter, Index 18 (95% over 21bp)
GGGAAGAACCAGCACGGTGAATGGAGTCTTGTTCAGGCCAGGGGACGTAGA11.694915254237288No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTC11.694915254237288No Hit
CTTATACACACCCGAGCCCACGAGACTCCTGGTCATCTCGTATGCCGTCTT11.694915254237288No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAACAAAAAA11.694915254237288No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCAATATCTCGTATGCCGTC11.694915254237288RNA PCR Primer, Index 37 (95% over 21bp)
ACCTCCAATAGAACGGGTTTGAATGGTAAGTCCATCTCCAATATCTGTCTC11.694915254237288No Hit
GCGGACAAGCGAAACATCGGCCACAACGTAAGGAGCCTGTCTCTTATACAC11.694915254237288No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.694915254237288No Hit
ACATTTATTTCCTATCTCTGCAACAAATACCACGCATCAGTTACATGTGAA11.694915254237288No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCC11.694915254237288No Hit
GGTATCAACGCAGAGTACGGGGTGGGGTCTACGCAGAAAAAAAAAAAAAAA11.694915254237288No Hit
TCGACGCAGAGTACGGGCAGTGGTATCACTGTCTCTTATACACATCCCCGA11.694915254237288No Hit
GGTATCAACGCAGAGTACGGGAGTCTGTCTCTTATACACATCTCCGAGCCC11.694915254237288No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTCCTGAATCTCGTATGCCGTC11.694915254237288RNA PCR Primer, Index 46 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTGT50.045.034
AACGTAA50.045.025
TATACAC50.045.045
GAAACAT50.045.011
AAACATC50.045.012
TGTCTCT50.045.038
CCTGTCT50.045.036
TCGGCCA50.045.017
CACAACG50.045.022
CTCTTAT50.045.041
GAGCCTG50.045.033
GCGAAAC50.045.09
ACGTAAG50.045.026
GGACAAG50.045.03
CGGACAA50.045.02
TTATACA50.045.044
ACATCGG50.045.014
GCGGACA50.045.01
ATCGGCC50.045.016
GTCTCTT50.045.039
Produced by FastQC (version 0.11.2)