Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_1228_pcontrolb_3.35100000042b81.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 184058 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC | 7434 | 4.038944245835552 | RNA PCR Primer, Index 27 (95% over 21bp) |
TCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGT | 611 | 0.33196057764400355 | No Hit |
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 346 | 0.18798422236468937 | No Hit |
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA | 281 | 0.15266926729617838 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCG | 259 | 0.14071651327299003 | No Hit |
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 256 | 0.13908659226982797 | No Hit |
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 244 | 0.1325669082571798 | No Hit |
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA | 244 | 0.1325669082571798 | No Hit |
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 243 | 0.13202360125612578 | No Hit |
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA | 214 | 0.11626769822555934 | No Hit |
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 199 | 0.10811809320974909 | No Hit |
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 186 | 0.1010551021960469 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TTATGTG | 20 | 7.009487E-4 | 45.01087 | 5 |
TGCCGTC | 775 | 0.0 | 40.93425 | 45 |
ATGCCGT | 785 | 0.0 | 40.412792 | 44 |
CGTATGC | 805 | 0.0 | 39.40875 | 41 |
GTATGCC | 805 | 0.0 | 39.40875 | 42 |
TCGTATG | 805 | 0.0 | 39.40875 | 40 |
GACGAAT | 800 | 0.0 | 39.37381 | 26 |
CTCGTAT | 815 | 0.0 | 39.20127 | 39 |
TATGCCG | 810 | 0.0 | 39.16548 | 43 |
ATTGCTA | 815 | 0.0 | 38.925205 | 31 |
CACGAGA | 825 | 0.0 | 38.726105 | 21 |
CCCACGA | 825 | 0.0 | 38.726105 | 19 |
ACGAATT | 815 | 0.0 | 38.64914 | 27 |
GTATAAC | 35 | 6.209424E-6 | 38.58075 | 1 |
CTATCTC | 835 | 0.0 | 38.262318 | 35 |
TATCTCG | 835 | 0.0 | 38.262318 | 36 |
TTGCTAT | 830 | 0.0 | 38.221737 | 32 |
CGAATTG | 830 | 0.0 | 38.221737 | 28 |
CGAGACG | 830 | 0.0 | 38.221737 | 23 |
GAATTGC | 825 | 0.0 | 38.180664 | 29 |