FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_3.35100000042b81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_3.35100000042b81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184058
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC74344.038944245835552RNA PCR Primer, Index 27 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGT6110.33196057764400355No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3460.18798422236468937No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA2810.15266926729617838No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCG2590.14071651327299003No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2560.13908659226982797No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2440.1325669082571798No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2440.1325669082571798No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2430.13202360125612578No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2140.11626769822555934No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1990.10811809320974909No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1860.1010551021960469No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGTG207.009487E-445.010875
TGCCGTC7750.040.9342545
ATGCCGT7850.040.41279244
CGTATGC8050.039.4087541
GTATGCC8050.039.4087542
TCGTATG8050.039.4087540
GACGAAT8000.039.3738126
CTCGTAT8150.039.2012739
TATGCCG8100.039.1654843
ATTGCTA8150.038.92520531
CACGAGA8250.038.72610521
CCCACGA8250.038.72610519
ACGAATT8150.038.6491427
GTATAAC356.209424E-638.580751
CTATCTC8350.038.26231835
TATCTCG8350.038.26231836
TTGCTAT8300.038.22173732
CGAATTG8300.038.22173728
CGAGACG8300.038.22173723
GAATTGC8250.038.18066429