FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_28.35100000042eb4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_28.35100000042eb4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC54.9504950495049505No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT21.9801980198019802No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTC21.9801980198019802No Hit
CTGCCCGTACTCTGCGTTGATACCACTGTCTCTTATACACATCTCCGAGCC10.9900990099009901No Hit
TATCAACGCAGAGTACGGGAGTGGTATCAACGCGTACTTACCTGTCTCTTA10.9900990099009901No Hit
CTCTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACTTGAAACA10.9900990099009901RNA PCR Primer, Index 23 (96% over 28bp)
CTCTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACAACCAACTAAG10.9900990099009901RNA PCR Primer, Index 23 (96% over 28bp)
GATATTTGCAAATGACGTATCAGATAAAGGGCTAGTTCTGTCTCTTATACA10.9900990099009901No Hit
CTTATCCACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC10.9900990099009901No Hit
CTAGGAATTCCTCGTTGAAGACCAACAATTGCAATGATCTATCCCTGTCTC10.9900990099009901No Hit
CTTATACACATCTCCGAGCCTACGAGACCCATAACGATCTCGTATGCCGTC10.9900990099009901TruSeq Adapter, Index 1 (95% over 22bp)
ACAAGATACTGGCAACTCTTGGTTTCACTGATGAGAAATCTAAAGAGTTAG10.9900990099009901No Hit
CTACAATCGTTTTCACCTACCAGTGCTTATAACTCTGTCTCTTATACACAT10.9900990099009901No Hit
ACTGTATCCTTCTCAACAATTTCTCACTTCATTGATCATTTCTAGTTGGAG10.9900990099009901No Hit
GGTGGGGACGGGCCCGGACAACCCGACTCACACAGACACCCCGACTGGAAC10.9900990099009901No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTACAAACTGTCTCTTATACAC10.9900990099009901No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA10.9900990099009901No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT10.9900990099009901No Hit
CTGGTGATCAGGTCGTGGGCCTTGAGCAGCCGGTAGACCGTGGCCTCGGAG10.9900990099009901No Hit
CACTCCTATTTCGCCAAGTCTCTCTCTGAGACACCATCCAGAACGTTCTGT10.9900990099009901No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAACTGTCTCTTATACAC10.9900990099009901No Hit
CCATACCAATCTTTTCTACTGAACCGTCCTTTCTTTTGCCTATACCATCAT10.9900990099009901No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATGCCGTC10.9900990099009901No Hit
TATCAACGCAGAGTACGGGCAACGCAGAGTACGAAAAACTGTCTCTTATAC10.9900990099009901No Hit
ATTTTACTTCCTTCGGGTCGAGAACTGAAATAAGTATCACTCTCTTCTGCT10.9900990099009901No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTCCTGTCTCCT10.9900990099009901No Hit
GAGAGGCAGAGGGAGAAGCAGGCTCCATGCAGGGAACCCAATGTGGGACTC10.9900990099009901No Hit
AGGCAAACCATCGCCCAAAGAAGGTCAACGCCATAATCACGATAGTCACCA10.9900990099009901No Hit
GTATAAGCTTAAGGATATTCAGACTAAGGTTGTGAAGGACTGTCTCTTATA10.9900990099009901No Hit
CTCTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACAAGAAG10.9900990099009901RNA PCR Primer, Index 23 (96% over 28bp)
CTTTATTACTATGAGCTCTAATTTTGGAAATCGTACGCATTCCAATGTATG10.9900990099009901No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA10.9900990099009901No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAAGCAGAAAAAAAAAAAAA10.9900990099009901No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCAAAATCTCGTATGCCGTC10.9900990099009901No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTCAC10.9900990099009901No Hit
TTGTATTTCTGAGCAAGTTCCCTGAAGCTGGTATTGTTACTGTATCTTTCC10.9900990099009901No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTGTTTATACCCCCCGTAATCCCCGTT10.9900990099009901No Hit
GTACTATTTCTCTATCGGTCACACAGTAGTATTTAGCCTGTCTCTTATACA10.9900990099009901No Hit
AAAAGTACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA10.9900990099009901No Hit
TATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA10.9900990099009901No Hit
CTTTTCTCATAAAATTGCTTCCTATTGGTCATTATTCACAAAGGTTTTTCC10.9900990099009901No Hit
AACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGCAGCTGTC10.9900990099009901No Hit
CTCTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGCAGGCCTAAC10.9900990099009901RNA PCR Primer, Index 23 (96% over 28bp)
GCTTTCACTTCCCAGCTGTATGCAACTATGCCAAATTGCTCTAAGTCTGTC10.9900990099009901No Hit
GTACTGGCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGA10.9900990099009901No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA10.9900990099009901No Hit
GTATCAACGCAGAGTACGGGGGTATCAACGCAGACTACTTTTACTTTTCTG10.9900990099009901No Hit
GTATCAACGCAGAGTACGGGAACACGTTAAGCCAAAATTACCTGTACCCGA10.9900990099009901No Hit
CTCTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACGTATGCCGGC10.9900990099009901RNA PCR Primer, Index 23 (96% over 28bp)
CAGAAGCTCTTTAGTGTAATTAGATCCCATTTGTTCTGTCTCTTATACACA10.9900990099009901No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC10.9900990099009901RNA PCR Primer, Index 46 (95% over 21bp)
ATATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAA10.9900990099009901No Hit
TATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAACTGTCTCTTA10.9900990099009901No Hit
GGGTCACATGGTAGTTCTACTTTTAGCTCTTTGATTAATCACCATACTGCT10.9900990099009901No Hit
CTAGTAGCTAGAAACTATTTTCCCAAAGCCTTCTTTATCGCATCATACTGA10.9900990099009901No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAA10.9900990099009901No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAGCAGAATCTCGTATGCCGTC10.9900990099009901No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.9900990099009901No Hit
CAATAAAACAAGTTGTGTAAAAAAAACCAAAACCAACACCCCTACCAAATT10.9900990099009901No Hit
CTCTTATACACATCTAGCCCACGAGACGGCATAGGATCTCGTATGCCGTCT10.9900990099009901No Hit
GGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACCACATATC10.9900990099009901Illumina PCR Primer Index 11 (96% over 30bp)
ATATTACGCTTCCTATTTCAGATAGCTGCTTAAAATGAGTAAGGAAAAACG10.9900990099009901No Hit
CTTGTATTAAAATGTAAGTTCCCTGAAGGCACATGATCACAATATCTATAT10.9900990099009901No Hit
GTATCAACGCAGAGTACGGGACGCAGTGGAATCCCGGCCCCTAAACACATC10.9900990099009901No Hit
TAGGACTACATAATCAAAACCGAGGACGACATGAACCCTGTCTCTTATACA10.9900990099009901No Hit
CTTATACACATCTCCGAGCCCACGAGAGACGCTATATCTCGTATGCCGTCT10.9900990099009901Illumina PCR Primer Index 11 (95% over 21bp)
GTATTTATCTGAAGGAGTTGAAAACATGTCCACACTGAAATCTGCACAAGG10.9900990099009901No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGATCTCGTATGCCGTCT10.9900990099009901No Hit
TTTCCATATCCCACAGCTTCGGTACGTCACTTAGCCCCCCGTCTGTTTCTT10.9900990099009901No Hit
GCTCAGCGGCGGCAGGATCGGGCCGATGAGCGACTGGATGGCTGTCTCTTA10.9900990099009901No Hit
ATCGAATTGACAAGAATCGAATCGAATCGAAATGAATCGAATCGAATTAAA10.9900990099009901No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGAGAATCTCGTATGCCGTC10.9900990099009901No Hit
TCCTAGGGGAGTTTACTTTTGTAGATGTGATTCAGCTCTGCAGAGGGAACC10.9900990099009901No Hit
GTATCAACGCAGAGTACGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA10.9900990099009901No Hit
GGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAAATACACAC10.9900990099009901Illumina PCR Primer Index 11 (96% over 30bp)
GTTAAACAGAGAAGTGATCTGAGGAGGTGAGCTCACTGTGATCACAACCAC10.9900990099009901No Hit
ATAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATCGTATGCAGA10.9900990099009901RNA PCR Primer, Index 33 (96% over 28bp)
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATCACATCTCCGAGCC10.9900990099009901No Hit
GAAATCAATATAGTGGACTGCTTACTACTACAGGCTTTATTTAAATACCAG10.9900990099009901No Hit
CGACAGAATGTTCAATAGAACGTCCGAAAAAAAAATCCGAGTAAGTTCTAC10.9900990099009901No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC10.9900990099009901No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATTCCGTC10.9900990099009901No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACACCTGTCTCTTATAC10.9900990099009901No Hit
CTTATACACATCTCCGAGCCCACGAACGTTAGGTCATCTCGTATGCCGTCT10.9900990099009901RNA PCR Primer, Index 7 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGTGCAGAATCTCGTATGCCGTC10.9900990099009901No Hit
CTTATACACATCTCCGAGCCCACGAGCTCGTGTGCATCTCGTATGCCGTCT10.9900990099009901No Hit
ATCCGGTGCCGCAGCGCCAACCACGCGAACAGCCGGTTTTTCTCCCCGAGG10.9900990099009901No Hit
ATACTATTAAGCCTGCTGTGATCATTTCCCATTTTGTGTCCGATAAAAGCA10.9900990099009901No Hit
AAAGTAAACTGCCATTGTATACACAGAGAAGAAGTCATGGTTGTGGTTTTC10.9900990099009901No Hit
CTTATACACATCTCCGAGCCCACAGACGGCATAGGATCTCGTATGCCGTCT10.9900990099009901No Hit
TTACTTAACCTAGTAACAAGTTAACACTTTTCTGACTGTCTCTTATACACA10.9900990099009901No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCCTGTCTCTTATA10.9900990099009901No Hit
GTATATTAGGTTCCTATAGCTCAAGTTGTATGGGTATTGTACCAACACCTG10.9900990099009901No Hit
AAAAAGTACTCTGCGTTGATACCACTGCCCAAAAAAAAAAAAAAACCTGTC10.9900990099009901No Hit
ATCCCAAGGGTTCCTCCACAAGGCTCGTCCAGGGAGGGTTAGTCAGGACCT10.9900990099009901No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers