FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_27.35100000042e9b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_27.35100000042e9b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC59.803921568627452No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTTCAGAATCTCGTATGCCGTC35.88235294117647RNA PCR Primer, Index 40 (95% over 21bp)
CTTATACACATCTCCGAGCCCCGAGACGGCATAGGATCTCGTATGCCGTCT11.9607843137254901No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC11.9607843137254901RNA PCR Primer, Index 46 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTTGCCGTATCTCGTATGCCGTC11.9607843137254901No Hit
CTGTGAGACTCAATTATATTATTGCAAGTATCAGCAAGTAATATTCCTGTC11.9607843137254901No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC11.9607843137254901No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCTGAATCTCGTATGCCGTC11.9607843137254901No Hit
AGAAAGCAGTCTTCAATCCAAACTATAGTATCTGGCATGTAACTGTCTCTT11.9607843137254901No Hit
CTTCAAATTTTTGAAAAGTTCTGTAGTATCTTCTCCGTTTTTCTTCATTTG11.9607843137254901No Hit
CTCTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACTCAAATCC11.9607843137254901RNA PCR Primer, Index 23 (96% over 28bp)
ATCACCTTCTTTGGTTAGTCTTTCCATACTATTCATCTCTTCTAGTACTCT11.9607843137254901No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGATCATCTCGTATGCCGTC11.9607843137254901No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA11.9607843137254901No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT11.9607843137254901No Hit
CTGTATGCCTCGCAGAACCGGTGCTGCAGGTAGCTCTGCTCGTCGTATGGC11.9607843137254901No Hit
CTTATATACTGAATCACATTTCCCAGTAACGTTCCAATCTGTCTCTTATAC11.9607843137254901No Hit
TCCCTATCCTATGCTTCTGGTCTTAATATAAGAAAGGGTGTAAGTACACAA11.9607843137254901No Hit
GTTGAATGAATGAATGAATGAATGAGTATCCTGTTTGGGGGCATTTATTTT11.9607843137254901No Hit
CTCTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACGTATGCCGAC11.9607843137254901RNA PCR Primer, Index 23 (96% over 28bp)
ATAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACACTATCTCTT11.9607843137254901RNA PCR Primer, Index 33 (96% over 28bp)
GATACCACTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG11.9607843137254901No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGAGAATCTCGTATGCCGTC11.9607843137254901No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTCCGCATCTCGTATGCCGTC11.9607843137254901TruSeq Adapter, Index 18 (95% over 21bp)
CTTATACACATCTCCGAGCCCAGAGACTCCTGGTCCTCTCGTATGCCGTCT11.9607843137254901No Hit
ATGGAATGGAATGGAATGGAATAGAATGGAATGGAATTAACCCGAATCTGT11.9607843137254901No Hit
CTACTATTCTCTCTACTACTTTGTTTCTAGCCACCTCACTTACTTTACTGT11.9607843137254901No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTATTTATTATAAA11.9607843137254901No Hit
CTTATACACATCTCCGAGCCCACGCACATAGCGATCTCGTATGCCGTCTTC11.9607843137254901RNA PCR Primer, Index 17 (95% over 23bp)
CTTATACACATCTCGGGGCCCACGAGACGGCATAGGATCTCGTATGCCGTC11.9607843137254901No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGGCTAATCTCGTATGCCGTC11.9607843137254901No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCCAGAATCTCGTATGCCGTC11.9607843137254901RNA PCR Primer, Index 46 (95% over 21bp)
GTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCA11.9607843137254901No Hit
TTCTGGACATTTCGTGTAAATGGAATGATACAGTATTTGGTCTTTTGTGTC11.9607843137254901No Hit
GGATCATCTCAAGGGGAATCTGAGACATGTTCTTCTCACTCTGTCTCTTAT11.9607843137254901No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC11.9607843137254901No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTAAAAAAAAAAA11.9607843137254901No Hit
CAGAATGGAATATTGACCTTAAAGTATGAGGAAATCCTATCATTTGCAACA11.9607843137254901No Hit
GCTTGAACCAGGATCTGGGAGGCGGAGGTTGCAGTGAGCCATGGTCACTGT11.9607843137254901No Hit
GAGTACGGGTGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAGAACTCACC11.9607843137254901No Hit
ACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTG11.9607843137254901No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAGCTTCATCTCGTATGCCGTC11.9607843137254901No Hit
CTTATACACATCTCCGAGCCCACGAGCGTTAGGTCATCTCGTATGCCGTCT11.9607843137254901RNA PCR Primer, Index 7 (95% over 21bp)
GTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACAC11.9607843137254901No Hit
CAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAGT11.9607843137254901No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGACA50.045.021
AGGGGAA50.045.012
AATCTGA50.045.017
TCAAGGG50.045.09
CTGAGAC50.045.020
ATCTCAA50.045.06
ACATGTT50.045.025
TGTCTCT50.045.042
GTTCTTC50.045.029
CATCTCA50.045.05
TGTTCTT50.045.028
CTCTTAT50.045.045
GGATCAT50.045.01
ACTCTGT50.045.038
AAGGGGA50.045.011
GACATGT50.045.024
TCTGTCT50.045.040
TTCTTCT50.045.030
GTCTCTT50.045.043
TTCTCAC50.045.033
Produced by FastQC (version 0.11.2)