FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_25.35100000042e58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_25.35100000042e58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC812.121212121212121No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTTCAGAATCTCGTATGCCGTC23.0303030303030303RNA PCR Primer, Index 40 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC23.0303030303030303RNA PCR Primer, Index 46 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGCATCTCGTATGCCGTC23.0303030303030303RNA PCR Primer, Index 43 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTTGTAGGATCTCGTATGCCGTC11.5151515151515151No Hit
TCTTATACACATCTCCGAGCACACGAGACGTTAGGTCATCTCGTATGCCGT11.5151515151515151No Hit
ATAGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACACACCCCCCC11.5151515151515151RNA PCR Primer, Index 33 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGGATCTCGTATGCCGTC11.5151515151515151No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCACTATCTCGTATGCCGTC11.5151515151515151No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTTCCGAATCTCGTATGCCGTC11.5151515151515151RNA PCR Primer, Index 46 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTTTCACAATCTCGTATGCCGTC11.5151515151515151No Hit
GGAAAGCCCGCTAGAAGGTTTGGGAACGAGGGGAAAGTTCTCAGAACTGTT11.5151515151515151No Hit
GTCGTATATAAGTTTATTAGAGGTAGAGCTACTGCTGTCTCTTATACACAT11.5151515151515151No Hit
CTTATACACATCTCCGAGCCCACGAGAACTTGACGTATCTCGTATGCCGTC11.5151515151515151No Hit
AAATTAAACAATAGGCTCCTGATGACCAGAAGAAATTAATCTGTCTCTTAT11.5151515151515151No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCATAATCTCGTATGCCGTC11.5151515151515151No Hit
ATCCATATCATGTGTAACAAAAATGAATGTCTTTCCTAATTGTTGTTGTAA11.5151515151515151No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCTAAATCTCGTATGCCGTC11.5151515151515151No Hit
CATTAAGACGATTGCTGAGTGTTTGGCTGATGAACTTATTAACGCTCATAA11.5151515151515151No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCCAGAATCTCGTATGCCGTC11.5151515151515151RNA PCR Primer, Index 46 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGCGCCCTATCTCGTATGCCGTC11.5151515151515151No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT11.5151515151515151No Hit
TGGTATTGTGGTTTGAAATTTTGTTTTCTACATGTGCATTGCTGACATATA11.5151515151515151No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCTGGATCTCGTATGCCGTC11.5151515151515151No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGTC11.5151515151515151No Hit
GGTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCT11.5151515151515151No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCTTTTATAC11.5151515151515151No Hit
ATGGTGTGCCTCCCTTCTTCCAGCGCATTGTAGTAGCTGTCTCTTATACAC11.5151515151515151No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAGCACAATCTCGTATGCCGTC11.5151515151515151No Hit
CTTATACACATCTCAGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC11.5151515151515151No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA11.5151515151515151No Hit
GTACTCTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCGCTT11.5151515151515151No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTTCTCGATCTCGTATGCCGTC11.5151515151515151No Hit
CAATACTACGGATAGTGATATTATCACTTCCACCTTCAATATTAGGCTGTC11.5151515151515151No Hit
ATACACATCTCCGAGCCCACGAGCGTTAGGTCATCTCGTATGCCGTCTTCT11.5151515151515151TruSeq Adapter, Index 7 (95% over 24bp)
ATCGTATATGTCCCAGTGACATGTATGTCAGCAATAACTTCATTTGATTTG11.5151515151515151No Hit
ATACTTATGTCATTGTGCTCATCACAGTCTGGTAAATGTTAGCATAGGACT11.5151515151515151No Hit
GTGCGCCGCAGGATCACGCCGTACGCGGAATCCGCGAGCGCTGTCTCTTAT11.5151515151515151No Hit
AGTCCATTCGATGATACCATTCAATTCCATTCGTTGATGCTTCCATTCAAG11.5151515151515151No Hit
AATCATGACTTTGAACATATTCTCTAACGCCTGTCTCTTATACACATCTCC11.5151515151515151No Hit
AGACTGACAATTTAGTTATGGTTACAAAACGGAAAACCATGCCCTGTCTCT11.5151515151515151No Hit
TAACAATACTCAGCTTTCCATAGAATGTTAGAATTTGTCCTTGATATGATC11.5151515151515151No Hit
CTTATACACATCTCCGAGCCCACGAGATCTTACTGGATCTCGTATGCCGTC11.5151515151515151No Hit
TTGTAAAGTCATCTTAATTCATTCCGACTTCATTTTCTTCATTCAGCCATC11.5151515151515151No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGTTAGATCTCGTATGCCGTC11.5151515151515151No Hit
CTCTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACCTGCCGCATG11.5151515151515151RNA PCR Primer, Index 23 (96% over 28bp)
GGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACACACC11.5151515151515151Illumina PCR Primer Index 11 (96% over 30bp)
TCTTATACACATCTCCGAGCCCACGAGACGTTGTAGGATCTCGTATGCCGT11.5151515151515151No Hit
ACACAATCCATCACATAAACAGAACCAATGACAAAAACCACATGATTATCA11.5151515151515151No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTGACGTATCTCGTATGCCGTC11.5151515151515151No Hit
GCACTACAGCAAAATCGTCAGCTAGTTTTTCATCACTGCGATAATGATGCT11.5151515151515151No Hit
GCGACCACCGAGATCTACACACAGGTATTCGCTGTCTCTTATACACATCTC11.5151515151515151Illumina Single End PCR Primer 1 (95% over 22bp)
TGAGTAGATAGCGCACCGGACCGAGCGTCGGCGTGGCCGGATCGGCGATCA11.5151515151515151No Hit
ATTCAGTGACTCTGACCATGTCTCAGTGTTTAAAAATGCTAATTGGTGTGT11.5151515151515151No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTACTGGATCTCGTATGCCGTC11.5151515151515151No Hit
TATCCATCCTTTGAGGATTTATTTCCATTACCAATTCTAAACTCCACCTGG11.5151515151515151No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGATT50.045.041
ACAAAAA50.045.031
AAACCAC50.045.035
CAATGAC50.045.026
CCAATGA50.045.025
AATCCAT50.045.05
ATCCATC50.045.06
CACATGA50.045.039
TAAACAG50.045.016
CACATAA50.045.012
ACAGAAC50.045.019
ACATGAT50.045.040
ATGATTA50.045.042
AAACAGA50.045.017
ATGACAA50.045.028
ACCACAT50.045.037
CACAATC50.045.02
TGATTAT50.045.043
ACCAATG50.045.024
TGACAAA50.045.029
Produced by FastQC (version 0.11.2)