FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_23.35100000042e14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_23.35100000042e14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61969
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC933615.065597314786425No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT10301.6621213832722814No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG3330.5373654569220094No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2950.4760444738498282No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA2700.4357017218286563No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2630.42440575126272817No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2150.3469476673820781No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2060.33242427665445623No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2050.3308105665736094No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2020.3259694363310688No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2020.3259694363310688No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1930.31144604560344685No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1750.28239926414820316No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA1700.2743307137439688No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA1550.25012506253126565No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1360.219464570995175No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA1260.20332747018670627No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1250.2017137601058594No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA1200.193645209701625No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1120.18073552905485No Hit
TTTTTCTTACTGATTGAGGATTCCTAATGTGATGAAAGCTAAATTAAAGCT1100.17750810889315627No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1100.17750810889315627No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1010.1629847181655344No Hit
TTTTTTTGGTTTACCTTCGTTTAATGCTTGTTGTTTTAATACTTCTCTTTC990.15975729800384064No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC980.15814358792299374No Hit
GTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA890.14362019719537186No Hit
GTATAAGACAGACCCAATTTACAGACTGTATGGAAAATTTGAACAAGAAAG890.14362019719537186No Hit
AGATTATTGACTAAAGAGACCTTCACTAAAGAAGAAAACAGTAGGATTAAA860.13877906695283126No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA850.1371653568719844No Hit
GGTTATTGTTATTGTGGTTTTGATTCTGAATCTGTCTACGAATTTGATACA820.13232422662944376No Hit
GATAATGTCATTGAGATGAGATGATGTTGATGTTGAGAATGATATTGAGAT800.12909680646775No Hit
TAGAGAGATAGAGAAAGAGAGAGAAGAGAGACAAATGGAGAAAGAGACAGA800.12909680646775No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA770.12425567622520939No Hit
AAGTACAACTGATTCATCATAAACTTTAGAACCAATGAGTACTCTAAATCC760.1226419661443625No Hit
GAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA730.11780083590182187No Hit
ATGAATGAGTAAATAACCAACGAAACCAATGATAAGATCAATGCATTTAAT690.11134599557843439No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAA650.10489115525504687No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA640.10327744517420001No Hit
TATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA640.10327744517420001No Hit
ACATATACATTCACACGTTTATAAAGGTATGCTTCCTTTTACAACTGAATC630.10166373509335314No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCATAT253.832182E-545.03153
CCATATA253.832182E-545.03154
CGTTTAA206.9853757E-444.99515518
TTGTTGT206.9853757E-444.99515528
CTCTTTC253.850535E-544.99515545
GGTTTAC206.9853757E-444.9951558
TACATAC206.9853757E-444.9951559
GTTTAAT206.9853757E-444.99515519
ACATACG206.9853757E-444.99515510
TCGTTGA206.9853757E-444.99515535
GATTCTT302.1338874E-644.99515516
CGTAAAC206.9853757E-444.99515525
ACGATTC206.9853757E-444.99515514
TCGTAAA206.9853757E-444.99515524
TGGTGCC206.9853757E-444.99515541
TACTTCT206.9853757E-444.99515540
CATACGA206.9853757E-444.99515511
CTCGTAA206.9853757E-444.99515523
GGTGCCT206.9853757E-444.99515542
GTGCCTT206.9853757E-444.99515543
Produced by FastQC (version 0.11.2)