FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_22.35100000042df9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_22.35100000042df9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences231290
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC183837.9480306109213545No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT48172.082666781962039No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG13250.5728738812745904No Hit
GTATAATCCCTGATGAATAAGTGTTCTACTATGTTTCCGTTCCTGTGTTAT5880.2542262959920446No Hit
GGTATAACACAGGAACGGAAACATAGTAGAACACTTATTCATCAGGGATTA2850.12322192917981753No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGGC253.8742848E-545.013412
TGCCGTC18000.044.36903445
CCCTGAT1400.041.7800948
TCCCTGA1450.040.3393977
GAACGTG301.1365568E-437.50306326
GATCCCC301.1380052E-437.49495342
CTCGATA301.1380052E-437.4949539
ACCACAG301.1380052E-437.49495339
ATCCCTG1600.036.5575836
ATGCCGT22150.036.056144
CTAAGGT250.002100561336.0107273
GTCGAGA250.00210279336.0029424
TGACTCA250.00210279336.0029431
AGGGTGT250.002105026735.9951557
CATTCGG250.002105026735.99515540
ACCCATC250.002105026735.99515542
GGGATTC250.002105026735.99515518
TAATCCC1650.035.465114
CCTGATG1650.035.4497769
CGTTCCT1750.034.71712538
Produced by FastQC (version 0.11.2)