FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_21.35100000042ddf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_21.35100000042ddf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84926
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC2931334.51593151684996No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT1765120.783976638485267No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG34464.057650189576808No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC4110.48395073357982243No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC2930.34500624072722136No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA1850.21783670489602713No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA1850.21783670489602713No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA1660.19546428655535406No Hit
GTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA1480.1742693639168217No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCC1400.1648493982996962No Hit
TTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT880.10361962178838048RNA PCR Primer, Index 27 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC29650.044.5446945
GCCGTCT301.1310406E-437.50000445
CAGAAGA250.002096159436.022
ATGCCGT48400.027.28822344
AAAAAGT2300.026.4130421
TATGCCG52200.025.30172543
CGTATGC52800.025.26988641
GTATGCC52400.025.2480942
TCGTATG53000.025.17452840
TCTCGTA53350.025.05154638
CTCGTAT53350.025.00937339
ATCTCGT53100.025.037
TATCTCG53150.024.84948236
TCCTATC52750.024.82464433
GAGACTA53600.024.8087724
CCTATCT53150.024.80714834
ATCCTAT52800.024.80113632
AAAAGTA2450.024.795922
AGACTAG53450.024.79419925
AGATCCT53000.024.79245430
Produced by FastQC (version 0.11.2)