FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_20.35100000042db5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_20.35100000042db5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124221
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC122479.859041546920407No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT14581.1737145893206462No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG3800.30590640873926306No Hit
CTATTCAGTTGATCATAAACAGAGAGTTAGGTACTGCTAATACGGAGAATT1690.13604785020246174No Hit
GAATGTAATGGAGTGTAAAGGAATTGAATAGAATCAATCCGAATGTAATGG1660.13363279960715177No Hit
TCAATGATCAGATAAAAGAACGAATTGATTTCGACAGCAAAAATAACTGCA1470.11833747917018862No Hit
CAATAGAAAGATGGATAGATAGTGTCCATGGTTAGATGAATGGATAAAAAA1450.11672744543998197No Hit
GTATCCAAAAGAAACAAGAGCAGTTTTTAACTCATTCTTATCAATGGTCCC1400.11270236111446535No Hit
CATTTCATTTAACAGCTACAGAATACATATTCTTTTCCTCAGGACATGGAT1400.11270236111446535No Hit
ATTTTCCTTAGTCAACTGAAGAGGACATAGTTCATTTAACTAACAACGAGC1380.1110923273842587No Hit
GTACTTCCTTACGTAGACATACCATAGTTAATTTAACCACTCTCCTATGTA1290.10384717559832879No Hit
GTGTTAAAGGAGATAAATCATCAAAGCTTAAATTTGCTTTCCGTATTTATG1270.10223714186812215No Hit
GTTCTAGATGTAGGTCTTTTATTCTATGACATGTGCAGTAACTTAACATTT1260.1014321250030188No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAACCA206.9848995E-445.0314254
CAGTGCT206.9848995E-445.0314254
CCTTACG253.8731294E-544.995177
TACCCTC253.8731294E-544.9951728
GACAAAT253.8731294E-544.9951713
GATTTCG253.8731294E-544.9951727
AAGAACG253.8731294E-544.9951716
TCCTATG253.8731294E-544.9951743
AGAACGA253.8731294E-544.9951717
CACTCTC253.8731294E-544.9951738
AACGCCA253.8731294E-544.9951740
CGAATTG253.8731294E-544.9951721
CTCCTAT253.8731294E-544.9951742
TACGTAG253.8731294E-544.9951710
CTTACGT253.8731294E-544.995178
GTGCCCT253.8731294E-544.9951733
CCACAAT207.0126925E-444.99516727
GAGTGCT207.0126925E-444.99516719
CAGCGCT207.0126925E-444.99516727
AATCCGA207.0126925E-444.99516736
Produced by FastQC (version 0.11.2)