FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_19.35100000042d9c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_19.35100000042d9c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290672
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC270229.296389057081521No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT53991.857420047338581No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG12840.44173501403643967No Hit
CTATACACATCTTTGAGTAAATGATGGACAATACATGAAGTACTACTAGAC3420.11765839158914516No Hit
CTTTTAATCTCTCATGTACCATTATTCCATGTACCTTCTGTCTCTTATACA3030.10424120658336544No Hit
AAACAGCTTTACTTTGTAGACCACAGTTAGCATTATCTTCAGTTAGGGTTT3000.1032091154290747No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGC253.8806105E-545.0058485
AGGCTGG253.8806105E-545.0058486
ATACACG253.8806105E-545.0058484
AACCGTG302.160512E-644.9981087
AACGTAC253.8845566E-544.99810824
GCGACTC253.8845566E-544.99810827
TCCGCTA253.8845566E-544.99810812
ACTGCCA253.8845566E-544.99810812
GGATGGG253.8845566E-544.99810821
TGGACGT351.2081364E-744.99810840
TAGGTCG253.8845566E-544.99810831
ATGGACG351.2081364E-744.99810839
GGGTAAC253.8845566E-544.9981087
TTGTCGT302.160512E-644.99810814
CGACATG253.8845566E-544.99810829
TTCCGAC253.8845566E-544.99810838
AATGCGC302.160512E-644.99810810
TACGCAG302.160512E-644.9981087
CTACGTG351.2081364E-744.99810826
GATGGGC253.8845566E-544.99810845
Produced by FastQC (version 0.11.2)