FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_18.35100000042d65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_18.35100000042d65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260779
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC162876.24551823574751No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT37661.4441346887594475No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG10800.4141437769145522No Hit
CTAAAATACTCCTTAAGTCTTGAACATTACAACATACCATAGGTTGTATGT4790.183680434390806No Hit
AGTGTATACTGTTCATTTTCTTCATACTTAGACCACAACACATGTACTTCT4750.18214656855038172No Hit
AGTTTTATTGTTGTTTGTTTGTTTTGAAACTACTTGCGATTTATCATTCCT4080.15645431572327528No Hit
ATCAATTATCTGTCTACCTAACATCTATTCCTGTATCTCTGTCTCTTATAC3850.14763458714083574No Hit
ATTACAGTTCAAGTTGATTTTATGAACACTAGATCCTTTGTATTGTCACCT3590.13766445917807799No Hit
CTTAAAAAGTGTTAGTGTTAGAGTACAAGGTAATAATGCATTTGGCTTGTT3580.13728099271797192No Hit
TGAACCTGATAATGGAATTGATACGGCTTATTATTCTGGACTTAGATTTGT3510.13459672749722945No Hit
AAACATTATTTTTCCTTCTCAACCTTGCATATGTTGGACGAATGAAGATTC3450.13229592873659307No Hit
GAGCATTTCAAGAGATAGAACATGTACGGATTTTGTATTACGATAGACACA3420.13114552935627485No Hit
AATTAACAATGAAATGCGTAAGTTTAGACATGATTACGTGAACATCTGTCT3390.12999512997595664No Hit
GTACTTGATCAAGTGATGAAGTTAAAAAGGCATTTAGATGTGTTGGACAAA3380.12961166351585057No Hit
ACCTTCATTATGTAGTCCTACACTCCTACTGTGTCTAGTATATGTCACAAT3370.1292281970557445No Hit
GAATGGAATGGAATGGAATGGTATGGAATGAAATCAACACGAGTTGAATGG3370.1292281970557445No Hit
CATTATAAACATTGGTGTTGATGATACTTTGATAGGTAATCATCTGTCTCT3360.12884473059563845No Hit
TCCTAATGGGAAAGACTACATAAATCCTACGTTTGATCCATCTACTAAAGG3260.12501006599457778No Hit
ATGTTTTCTTTACTTGGTTTGTCGATAGTATTCTGTAATACTTCACGCAAC3220.12347620015415353No Hit
ACACTAGAATATGATGTAGTTTGTAAATCCACCATACTAAACTGTCTCTTA3210.12309273369404745No Hit
GGATATTTTGATTGCAGATACTTTTGTGGCTTCATTTCTCATTCCATTTGA3210.12309273369404745No Hit
AAATAACATCTTCTAAGTACACAAATTTCCACCCAGTGAGTAAAGAACGAT3170.12155886785362319No Hit
GTTAATCAACAAGGTTACAAATCAAATACTGCAGAATTGGTTATGATGAAA3160.12117540139351711No Hit
ATATAATCACCTTTGTCAATGAAAACAGATCATATAGCCTGTCTCTTATAC3150.12079193493341105No Hit
GATTTATTCAAACAAAACAATGGTTCAACTGAAGAAAAAACTGTCTCTTAT3120.11964153555309284No Hit
TTCATAAACTTTGTTATTTGCTTCTACTGTTTCTTTTCTGTCTCTTATACA3090.11849113617277465No Hit
CATTAGTTAAAAGTAGGAATATGGAGTACCATAGCAGGTGTATCTAAACTA3090.11849113617277465No Hit
TTAAGAAGTAGCTTTCCTCTTATGTGTTCATTCAAAGGTTCAATAGACGAT3070.11772420325256253No Hit
AATGACAAGTGACTTAACATTACGTTTCCAGATACCAAAATACCTAATCAC3050.11695727033235039No Hit
CTAGTAATTCCATTAAGCCATAAGAAGGGAAAATTTAATCACTGTCTCTTA2990.11465647157171398No Hit
GATATCTTGATTCCAATTCTTTTCCTCATTTTTCATTCTCCAAAATGTGGT2960.11350607219139579No Hit
TTATAAGCCTTATCTTAGAGATTACAAAGGACCTGTTTGTTACTGTCTCTT2950.11312260573128972No Hit
TCTCAGATGAATTAACTACCTAATCCTGAAGATATACAACTGTCTCTTATA2940.11273913927118365No Hit
GATGAGTTAAGGGATAAGATACTAAGACTAGCTGAGATTAATAAACCTGTC2910.11158873989086544No Hit
CTAGAAATACCTTATACAGACAAACTCAGATGCAGAATAACTCAAATCCTT2890.1108218069706533No Hit
TTTCTTAAGAAAATGTCAAAGCCAATCATGTTCATGCAGGTTATAGCTAGT2880.11043834051054724No Hit
GATCATGTGATTTGATACAATTTAGTTCTTGCAGTGTATAAGCTCAATAAC2880.11043834051054724No Hit
TGAAAGGTCAAAAAAGAAGAAGAAGAAGAAGAAAACTACGAATCTCTATAG2880.11043834051054724No Hit
AGAGATGTATGAAAACTCTTGTGTAAGAAGTCTGTTATAATCGGATATTTT2870.11005487405044118No Hit
GATATATGGTCATTTGAGCATAATGTGCTTTTTAATACTCTCTTCAGACTG2830.10852100821001692No Hit
AAAGTAGTCATAACATATGACAAGGAAGATCTTGTGTTATCACATACCCAA2790.10698714236959264No Hit
GGATATGCCATTATTAAATACCAAAGAGCAAGATGATACTATGAAGACTGT2780.10660367590948658No Hit
ATTTACTTTAGACCTTGGTACCTGAACTTTAATCATTCGTAGAACTACTCT2760.10583674298927444No Hit
AAATTGTACAATGCTTAATTCTCAATGCTCTGTTTATACCTGTCTCTTATA2740.1050698100690623No Hit
TTTGTTACTTGTTTCTAGTTTCATTGCATTGTGTTCAGAAAACAGTGTATC2730.10468634360895623No Hit
ATAAAAGAGGATACAAACAAATGGAAGAACATTCCATGCTCATGGATAGGA2720.10430287714885017No Hit
CGATTGAATATGATGGAGAATATGGTAAATACGTAATTACACGTATCGATA2690.10315247776853198No Hit
TCTTGGTATTGTTTCAATTTACACTAGTGTATTGAAACGATGATTGAAACT2680.10276901130842592No Hit
TATTTGGATAGCTCTAATGATTTCGTTGGAAACGGGAATATCATCATCTAA2670.10238554484831984No Hit
ATTGAGGAGTAATCCTAAGATATGGAACATACATAGTGCTGTCTCTTATAC2670.10238554484831984No Hit
CATAGATACACATGCATACACATATCGTCATAGATACATACATAGATCCTG2640.10123514546800164No Hit
ACATAATTACTCACTGAGCACTTATTAGGTAATATGCTGAGAGCTTTACAA2610.10008474608768343No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTACG253.8781858E-545.008441
AACGTCA302.1590895E-644.9998137
TGGACCT302.1590895E-644.99981333
CCCAGTG302.1590895E-644.99981332
TCTAGCC302.1590895E-644.99981337
TACATCG302.1590895E-644.99981321
GATTGTC302.1590895E-644.9998139
TTGCCTG302.1590895E-644.9998139
ATAACGT302.1590895E-644.9998132
CTAGACT253.8825823E-544.9998115
GACAGGA351.207136E-744.9998111
CGAGTTA502.1827873E-1144.9998114
CGAAACG253.8825823E-544.9998145
ACGCATG253.8825823E-544.9998133
CCTAGGA253.8825823E-544.9998111
ACTTGCG650.044.9998132
ACACCTC253.8825823E-544.9998114
CGCATGA253.8825823E-544.9998134
CCTACTC253.8825823E-544.9998142
GTCTACC351.207136E-744.9998112
Produced by FastQC (version 0.11.2)