FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_17.35100000042d4c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_17.35100000042d4c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248477
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC188527.587020126611316No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT41041.651661924443711No Hit
GTATTATTGCACATAGTTGTTACAAGACTTCCGTTGATAAATCAAAAGAGA15360.6181658664584649No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG11460.46120968942799534No Hit
GTGTAAGTTACATTCTTTACCTCAAGTAGTAGAGAAGATGTAGAGGAGAAT9710.3907806356322718No Hit
AGATAAAAGACATCAAACATAACCAACATGAAATCAACTAGAACTCAAAAC9360.3766948248731271No Hit
TGTTAAGAGTGCTTAGATTGGTAAGAAAACACAAGATAGTGATACTGTTGT7990.32155893704447497No Hit
TACTGTAACATCATTTTTCTTCTGATCTTAATACCACAATGATCACTGTCT7770.31270499885301256No Hit
ATTCTATTCTTTTCTATTCCATTCGAATCGATTCGTTTTGATTGAATTCGA7740.31149764364508586No Hit
GTACACTTATTTCTGTTTGTGGCTTTTTTCTTTTCTCTAGAGTTGTATCAA7090.2853382808066743No Hit
ACTCATATTCTGTTGATTTCAGTGGCAACAAATATTTCCTTGTAGTAGTAT6750.27165492178350514No Hit
GAATGGAATCATCATCAAATGGATTCAAATGGAATCATTTTCGAATAGAAT6560.26400833879996943No Hit
ATATACACTAAACCTGTAACTGTAAATGAGTGAATTCTGAGCAATGTCTTT5980.24066613811338675No Hit
TATTAGTATTGGTTGGTATTGAAACAACAGATTCTGAAGAGGATATACTGC5950.23945878290546008No Hit
GATTTATTTCAAGGGGCTAAGTATTTCCGAGTTAAGATGGATATTAAGCTC5860.23583671728168001No Hit
TTGATAGAGTTTAGGAAGTAGATAAAATGTAGAACCTGTCTCTTATACACA5810.23382445860180215No Hit
ATTCGATTCTTTTCAATTCGTTTCAATTCGATTCTTTTCGATTTGATTCGA5740.23100729644997325No Hit
ATACATTACTACTAAATCGATACAAGAGCTCTATGATTTCTGTCTCTTATA5580.22456806867436424No Hit
GTGTGTATTCATCTTACAGAGTTAAAACTTTCTTTGGATTCAGCAGTTTGG5560.22376316520241313No Hit
ACACTAAATTGTGCATGTATAACCTAAAACTCCACAAGGCTAAGCAAAGAT5520.22215335825851085No Hit
GGATATTTTGATTGCAGATACTTTTGTGGCTTCATTTCTCATTCCATTTGA5450.2193361961066819No Hit
GCTATAAACATGTGTGTGCAAGTATCTTTTTTGTATAAGGCTTCTTTTCTT5230.21048225791521952No Hit
ATATCTTGTGCAATGTTATGTATTGTTCTGTGCAATATCTGTCTCTTATAC5190.2088724509713173No Hit
AGACAAGGTAACATTAATAAGAACAAGCAGAAACAACCCAATATAGAAACT5190.2088724509713173No Hit
GTGTTAGTACCATCAATAACATTAGGATTAGCAGTCTCCATTACAAGCATA5090.20484793361156164No Hit
GTATTAGGTATAGTAGAAAGATCAACGGGGAATGTAAACGTTAAAGCATTT4950.19921360930790374No Hit
AGACTACGTTGTATATGAATCTACTGTTTATCCAGGTTGTACAGAAGAAGA4790.19277438153229473No Hit
CATTTCATTTCACCATTTCATTTCATCATTTCATTTCATCATTCCATTTCC4690.1887498641725391No Hit
GTAAGGTATTTGAAAAAAATCCGAATGGATGGAATGATAGTTTACGTAATA4660.18754250896461241No Hit
AATATGGGGATTTCCTACAGCATGAAAATTGTGTAATTGACTAAACAAATT4640.1867376054926613No Hit
ACCTTAGCTAACTATCTTAATTTCCAGTATTTACAACTGGATGAACTGTAT4630.18633515375668572No Hit
AGGTAGAAGAAAACAGGAACCATTGAAAATACAACATGAACATAACTGTCT4460.17949347424510115No Hit
TCACTAATCTATCGTTTCATCAGAAGTTCGGTTTTGAATAACATAGTGATG4380.17627386035729667No Hit
GAGTACGGGATTCTATTTGAATAAGACTTTTGAGTCACATATAACACTTTA4350.17506650514936997No Hit
GCTTTCATGATCTTTATTGTTAACAACAGAGTACCTGTAAGTACAAACGAT4340.1746640534133944No Hit
TATCTGCATTTGATCAGCATCTTACAGTGTCTTCGTTTTTTCTCTTTTGAT4330.17426160167741883No Hit
TAGTATGTTTGTCATTGATAACAAAACCTTTCCGTATATGAAACTGTCTCT4320.17385914994144328No Hit
TAGAGGTAGTATCCGAAATTGAATTTGCATACCGACATATATCAGGTTCTA4300.17305424646949216No Hit
GAATCAAATTGAAAAGAATCGAATCGAATTGAAAAGAATCGAATCGAAAAA4290.1726517947335166No Hit
GTATTGCCTAGGTTTTCTTCTAGTGTTTTTATGGTTTTAGGTCTTACATTT4240.17063953605363877No Hit
ATTTATTTCAAGGGGCTAAGTATTTCCGAGTTAAGATGGATATTAAGCTCT4220.16983463258168763No Hit
GCTATTTTTCATTCCTTGTTTCTTGCTCATTATTTTTTGTACTGAGCTGAA4210.16943218084571207No Hit
GTAGCTAAGAATGTGTGTTTTGTTTGAAAATTTTATGTTGACACAGAGAAA4200.16902972910973652No Hit
GATAGGAAATATCCAATGAATGCAGAAAATCAAACGTTTCTTTAAAATGTA4180.1682248256377854No Hit
ATTCTATGGACATGTTAACAGAAGATTATAATTCCTGTCTCTTATACACAT4160.16741992216583426No Hit
GACTTACTAATGCCATCTTCTTGTCTTCTTACTGTTTTGTACTTCTACTAT4150.1670174704298587No Hit
TGTTTGTGTATCTTCTATTGAGAATGTTCTATTCACATCTTTTGCCCAATT4100.1650052117499809No Hit
GAATTAATCGAGGCTTTAAAAATAGGTGAAGAGAATAAACAAAAAGCTATT4100.1650052117499809No Hit
CTAGTGATTTGAGCATCTTTTATGTATTTGTTGACCATTTGGATGTCTTCT4060.16339540480607861No Hit
GAATGGCATGGAATAGAAAGGAATTGAAGGGAGTGGAATAGAATGGAATGG4030.16218804959815195No Hit
GTTTTAGTCTTACTAAAGACTGATTTTATGCGAATTACAGAAACAAATACG4020.1617855978621764No Hit
CTTAAATCGAGTATCTGATGAAAGTGATGTTAAAAATGCACCTGGTATTAA4010.16138314612620083No Hit
TACATATACAGGGTACATGAGATGTTTTGATACAGGCATGCAATGTGGAAT4000.16098069439022525No Hit
TTCTTGGAGCATGTAAATCTGTTACAATGAACATGTCATTTTTATAGTCCT3970.15977333918229855No Hit
GTTTGTTTGTTTGTTTCATTTCTTAGCATTTATCTTACATCAGATTTACCT3970.15977333918229855No Hit
ATTCCATTAGTCTCCATTCAATTCCATTAATCTCCATTCCATTCCATTCTA3950.15896843571034744No Hit
TGATAAGTCTGGTAATCTACCATTAATCCATGTGCAATCAATTCATACCAA3940.15856598397437188No Hit
CTAATAATCAAATGGAATGAGAAATGAAGCCACAAAAGTATCTGCAATCAA3940.15856598397437188No Hit
CTCTTGAAAAGAAAATTAACCAAGTAAGTATCTCCTCTAGTAAGTCTGTCT3940.15856598397437188No Hit
ATTATATTGCCATGTTATCTTAATGCACCTATATACCTGTCTCTTATACAC3940.15856598397437188No Hit
ATTCTATTGTGACCATTATCAGAGATATTCCGGATGCAAAGGAAAGTGGAC3830.15413901487864068No Hit
ATTATATTGTCTGTTTTGTCAACACGAAAAATACCAAACTTAGAGGCTGCA3770.15172430446278728No Hit
CTTTATAAAGTAATCGCTTTCTTTTGTCTGTTTTCTTGCGATTTTTGTGAT3740.15051694925486062No Hit
CTTATGGCTTTATATCTGCCTATACCTTTTGTTTCTGTCATAAATACATCT3720.1497120457829095No Hit
TATTTATCCTTCACAATGCAGACGAAGACGTTCTAGAACTAATAAAAGAAC3670.14769978710303167No Hit
CATTTAAAGAACCAACCAAATGATGTTTCTTTCTCTCACTCTCTGTCTTTC3670.14769978710303167No Hit
TTTTGATGCTATTGTAAATGCAATTGTTTTCTTAGTTTCAATTTTGGTTTG3580.1440777214792516No Hit
ATATAGAAGTGTTCTAGTCTATGATGCATTTAGATTTGGTACCTGTCTCTT3570.14367526974327605No Hit
TTGAATGAGGTATTTCAGGGACTTTTTCAGCTATTCAAGTGATCACAAAGA3560.1432728180073005No Hit
AATCATTACAGTAAAAACATTTCTGTGTCACTCTGTCCTTGAAATAGCACT3530.1420654627993738No Hit
CTGCATTACCTTGAAAGTTTTGAAGTTCAAGCATGGTGTTTTCAAAGTTCA3520.1416630110633982No Hit
GATTTAAGATGGACAACTTCATCAGAAAACGGCATTAAAAATTATGTCGTT3520.1416630110633982No Hit
ATCTTATGTTAATTCACCAAGATGATTGGTTACATGATCTGTCTCTTATAC3510.14126055932742265No Hit
GTTCTATTGGAAAACCTTTTAAGCTACCTTAACGAGAAGAAGAGGTCATTT3500.1408581075914471No Hit
TATTTATCTTGATCAGCTAAAACTTTTTTCAAAGTAACACCTGTCTCTTAT3470.1396507523835204No Hit
TTCTTAAAGAGTTCCTTTGATGGAAACAAAGTAAGCATTTTAGATCAGAAG3450.1388458489115693No Hit
GTCTTCGTTTTTTTCTCTTTTGATTTATCAACGGAAGTCTTGTAACAACTA3450.1388458489115693No Hit
ATCCACATGGTATCATCAACTCTTTCTCTTCGATCATTGAAAGTAAGATAG3450.1388458489115693No Hit
AAAATTCACTTGGAATTCATGTTTTGTGATACGTTAGATTGGAATCCATTT3420.1376384937036426No Hit
TTTCTATTCTATCTTTGTGTTCACAATCTCCACCTGTAATTCTTAGTAAAG3390.1364311384957159No Hit
ATATTTACGTTTCTTTGGAATTGGAGAATCTAGATTAGTAACGGATATAGC3380.13602868675974034No Hit
GTCCACACTGATAGATAAAGACTTACACCAAATGTTTTGCATTCAAATATG3370.13562623502376478No Hit
GATATTACTGTGACGAATTCCATTTGCTTTTAAGAGAAGAACAAACAGCCT3350.13482133155181367No Hit
GAGTAATTCATTCTCTAACCAAACTTTGGCTCAGATTGAATTGTGGACTAA3340.13441887981583808No Hit
CTTAGCTACTTAGTAACATTCATAGAGCTTAATATCTGTCTCTTATACACA3310.13321152460791139No Hit
CTTAATACTCTGGTTTGCTTTTTTGTTCACGTTTAGTTTGCTTGTTTTAAT3300.13280907287193583No Hit
TATTAGTGTTACATCATTGTCTTCTTGTTTTACTTCAGCTTTACCACTGTC3300.13280907287193583No Hit
TCTTACAACAACACACAAAGCAAAAGATAAAGAAGCATTGTAAAGGTAGAT3270.13160171766400916No Hit
TATGTTGGGTGATAAAGAAACAGTATACGATCAGAAGCATACCGTTGTAAA3260.13119926592803358No Hit
ATCTTGATACACTTGCTTATATTCACCTTATGCTTCAGTCATGTCGTTTAA3260.13119926592803358No Hit
GTATGGTTTTTGTTTTTGTAGTGTGGTATGTTTGTAGGGTTAAGTTTTTTT3240.13039436245608246No Hit
ACTTACAACCAGACACACTAATATTCCTACAGTGGAACATAAGAACAATCA3220.12958945898413132No Hit
TTGCAGGATATTGTGGAAACATTGATTATTGTAATCACTGTCTCTTATACA3210.12918700724815577No Hit
CAATAGAACAAATAGACACAAAATCAGTAAGGATACAGAAGACTTGAACAA3150.1267722968323024No Hit
GTATCAAAGTAAGTAAGATCTTATGCGATTAAGATCCTATGTATCTGTCTC3150.1267722968323024No Hit
GTGCAATTTTTCCATTGCTTTTTTACAACTTCAATTGCAACATTTGATTTA3130.12596739336035126No Hit
ATGTCCTCATATGATCCAATACACTATTCACGTCATTCATAACATTTTTGC3120.12556494162437568No Hit
ACAGAAATTTTTCAGAAAGAAGGAAAAGAATATGTCAGAAACTCAGATCTA3120.12556494162437568No Hit
AGTTTCTTGGCAATAACTTGCTCTAATTGTTCATTACTTTGGTTATTAAGT3120.12556494162437568No Hit
AATCTAAAATGAACATGTAAATGAAAGGATACAACTCCTGTCTCTTATACA3090.12435758641644899No Hit
ATACATGTTTTTCCTGTACACATACCCATGATAAGTTTTGATAAACTAGGC3080.12395513468047345No Hit
TACCAATGTTGGTCGTCATCATAAAATAAAAGCCGGAGATACCATTGTAAC3070.1235526829444979No Hit
ATTCTAACTCATCTTCTTCAATATGTCTAAGGTTTCAGTAATTCTGAACTA3060.12315023120852231No Hit
GAATTACTCAGTGAATGATACATAGAATTACAGGATACCATACCTGTCTCT3060.12315023120852231No Hit
CTATTAGCTTAAGTTTAGAGTTCTAATTCTTTTTACTGCTCAATCTCTGTC2970.11952816558474225No Hit
TGCTAAATCATTTCATGTATTGAAAGAATTGCTATTTCCAACAATCCAATG2950.1187232621127911No Hit
GAGATACTAACGATTCACGATTCTATCAGTGTGCCATATCTGTATGTTGAA2950.1187232621127911No Hit
ATCAAATACATAGTCAAACTGATAACCTTCTCGGATAAATAGGTCTCTGAA2950.1187232621127911No Hit
GATTGTTGCATCTCATTTTGTTAAGAAAGGCTATTAACCTTTTCTTTTTCT2920.11751590690486442No Hit
GCTTAGTGTATTTGAAATAGCTATTTTCTCACTGTGAAAAAATAAGCTGTC2920.11751590690486442No Hit
GTTAATTTCCTGAAGATCTACTTAATCTGTGAAATACAAATTGTATCTGCT2910.11711345516888887No Hit
TAATACAGCTGTGCCTATTATTTTGGTAATCTGAAGCATGGAAAATCTTCC2910.11711345516888887No Hit
ATCCATTGCTTTATCTTTTTTGCTAAAGGAGAAGAAATGTTTTGCTAATGT2900.11671100343291331No Hit
GTTAAATGTAATTGTTCATTGTAGTTTCTTGTGATCCTTTTTATTTCTGTG2900.11671100343291331No Hit
GTTATTTTCAATAACATGCACCCATACCTAATAAGGTTTATCACTGTCTCT2880.11590609996096218No Hit
GTTTGGAATAGGTATCAATTAACGAATCACAAAACAAAGATAGAAATGTCT2880.11590609996096218No Hit
AAACTGTATTCTTCTCCAATTTCGAATTCATCTTCCTCATTGATTTCTGAC2880.11590609996096218No Hit
GATTATTGAAGTTACTGGAAGACTTCTATAAGAAGAGATATCCACATTCAT2880.11590609996096218No Hit
CTACTACAGAAAAAAGATATGATCAATAGAAAGCGCACCATAATTAGATGC2870.11550364822498663No Hit
AAATAGGGGAGAGAATAAGGGTTAGAGAGAAATAGATGAAATGGAATTTCC2860.11510119648901104No Hit
CTATACATCAGTATTCTCTTTGTGGAAACTGCTATTCCTCTAATTACTCTA2850.11469874475303549No Hit
CTATTGTTTTGTGTAGCATACCATTCTTTCTAGTGTTTATTCCTGTCTCTT2810.11308893780913325No Hit
ACACTCTTCTTACCCTGTTATTCCACTCACAAGAAACAATCTTAGGAACAT2810.11308893780913325No Hit
TTATCATAAGCATAAAACTGCTGCATATTCCCAAATTATTTCTACTTTGCC2810.11308893780913325No Hit
AAGTTATGCACCATAAGTGTGGTGTAGTATTCTTCATAATCTGTCTCTTAT2800.11268648607315768No Hit
AAGTTGTACTAATTGCATTATCCAACTGTTGAAAGAACTGTTAAGTCTGTC2790.11228403433718212No Hit
TTATTGGTTCATCATTTAGTCTTTTTACTTGAGTAGCTGTCTCTTATACAC2770.11147913086523098No Hit
TAGAAAAAAAGCAAATATAGGAACAAATTTGTGTCCTGGTTACTAAGAGGA2770.11147913086523098No Hit
GACTTGTGGATTTGGTATTATGCATATTTGAAGATGTTTCTTCTTCCTTAA2760.11107667912925542No Hit
ACTGAGTACTTCTAACATTTGATCATCTTTCAAATCGGAATAGTCGATTCT2760.11107667912925542No Hit
AAAACAAACAAACAAACCTAATTTGTTGATACGTCTATGTACCTCATAAAA2760.11107667912925542No Hit
ATATAATACATCGCTGCCTGAAAATAAGGATGATTGTCTTTGTTCATTACA2750.11067422739327985No Hit
GTAAGATACATTAGAAGTGAAACATAATCCAAAACACGTCTCAAACTCAAG2740.1102717756573043No Hit
AAATAAGGCATGGAGAAAAGGAATGTAGTACACTAATAATCAAATGGAATG2730.10986932392132874No Hit
GAAGATAATGGAGACATTTTCGTTTTTCAAAAAATAACCAAACATAGACTC2730.10986932392132874No Hit
ATATAATCCTGAGGTTACATGTTTTTAGAGCAGTTTCTCTGTGATTCTTCT2710.10906442044937761No Hit
GTGTCAACATAAAATTTTCAAACAAAACACACATTCTTAGCTACTTAGTAA2710.10906442044937761No Hit
GATCAACACGATATACCAGACATGAAAAGTATACATTTGCACAGTAATGAT2700.10866196871340206No Hit
TTTTATGGTAAGTCTGTTCATTCCAAATTTCTAATTGATTTGATTCTGTCT2700.10866196871340206No Hit
ATGCAAAACAGGAAAAAAAATGAAAGAAAAAGACTTCCAAAAACAAGAACA2690.10825951697742647No Hit
CTCTAATACTTTTTGCCCTTTGTATTTAGTTGCTATGTTATTTGGTACATA2690.10825951697742647No Hit
ATTGACAACTGTGTCTGAATCTGATAACAAAATATGAAAGCCTTAAATTAT2680.10785706524145092No Hit
GATTACATTCATAAAATCACAACCGATATGGTTAAAAGTTATGACGCTGTC2660.10705216176949979No Hit
AATCTAGAGAAACAGAGGTATTTTATGATCATGAACACGAAAAAAATCTTG2660.10705216176949979No Hit
AATTAGCTTCATTTTATGGATAAACTGAACTGAGGTATATCTGTCTCTTAT2650.10664971003352423No Hit
ATGGAGACCATAATACAATGAAGCATAAATAACATGACTGAAATCATGTCT2630.1058448065615731No Hit
TAACATTACCCGATGTTAAGTTAGGGAACATAAGTACATTAACATCTTCAT2600.10463745135364641No Hit
TTGATAATAGCAACTAGAATTCATTTAGAGTCTTTGTTCCAAGAACAAATT2590.10423499961767085No Hit
ATGTAAATCATCAGAAGAAACGAAATGTTTCCAAACCTGTTTGATGATCTC2570.10343009614571973No Hit
GTGTAATATAGGCATCATTAGTTTCTACCAATCGGCTAAGTTCACCGAATG2570.10343009614571973No Hit
GGTAAAAGCAGTACTCAGAGGGAATTTTATAGCAATAAATGCCTACATCAA2560.10302764440974417No Hit
CTCTAGTATCCTCTGTTCTACTTTTTATTTCCATGAGAACAACTTTTTAAG2550.10262519267376859No Hit
GCAATAAATAAGATACTTGTTTGTTCCTGCTTGATATGTGAGGTTATTATC2540.10222274093779303No Hit
AGAGTCTTTTTTGGTCAGAACAAACTGAGAGTTATGTTTTTGTTGTAAGAA2540.10222274093779303No Hit
AGCAAGCATAGATGTTATTCTACACAACAAACACTTTACATACTGTCTCTT2530.10182028920181747No Hit
CAGATTATCTATCTGTGATTATCGATCACAGATTATTAACCTGCTTACCTG2530.10182028920181747No Hit
CAAAAGTATGTATCAAACAACTACAATGATAACACTTCACATTCTGTCTCT2510.10101538572986635No Hit
ACTTGTGTAAGGAAACATAGGTTAATTTACTTTTACACCTGTCTCTTATAC2500.10061293399389079No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATAT253.8694543E-545.024571
ACGCAAT302.1505111E-645.0245671
CTATTAG453.8016879E-1045.0245671
GGCATAT207.0037396E-445.0245671
TTGCAGG207.0037396E-445.0245671
TGGCATG351.2035343E-745.0155074
GGGCAAT351.2035343E-745.0155074
CCACACT351.2035343E-745.0155073
AGCACAG302.153496E-645.0155035
CCTTACA207.0106925E-445.0155032
TCTTGGC502.1827873E-1145.0155035
CTCGTAG207.0106925E-445.0155033
TTGCTGG207.0106925E-445.0155032
GGGTAGT253.87406E-545.0155036
AGGGTAG253.87406E-545.0155035
TAGTTCG253.87406E-545.0155035
AACGGAG253.87406E-545.0155035
GCACAGA302.153496E-645.0155036
TCAGCTC253.87406E-545.0155033
CGTAGAG207.0106925E-445.0155035
Produced by FastQC (version 0.11.2)