FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_13.35100000042cc3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_13.35100000042cc3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50589
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC875617.30811045879539TruSeq Adapter, Index 12 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT647712.803178556603214No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCG6791.3421890134218901No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC2940.5811540058115401No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC2640.5218525766470972No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA920.18185771610429144No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA780.15418371582755144No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA750.14825357291110716No Hit
TTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT710.14034671568918144TruSeq Adapter, Index 12 (95% over 22bp)
GTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA680.13441657277273716No Hit
AAAAAGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACG560.11069600110696No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA520.1027891438850343No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10000.043.64904445
GCCGTCT301.1247024E-437.49917645
GTTGACT250.002088375235.99920737
TTACACA250.002088375235.9992074
ATATCCC352.7830145E-432.1421474
GTTTCTC300.005101928529.99934221
ATGCCGT16450.026.39759444
TATGCCG17000.025.54355643
GTATGCC17100.025.3941842
CACGAGA17400.025.3442721
ACGAGAC17400.025.3442722
CGAGACT17350.025.28762823
CTTGCAA17300.025.2306631
AGACTAC17400.025.21496225
AATCTCG17050.025.20472336
ATCTCGT17050.025.20472337
CCACGAG17500.025.19944620
ACTACTT17500.025.19944627
GACTACT17500.025.19944626
CAATCTC17150.025.18895135
Produced by FastQC (version 0.11.2)