H23LNBCXX_l02n01_bir_1228_pcontrolb_11.35100000042c9d.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l02n01_bir_1228_pcontrolb_11.35100000042c9d.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	39791
Sequences flagged as poor quality	0
Sequence length	51
%GC	37

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC	7254	18.230253072302784	TruSeq Adapter, Index 12 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT	534	1.342012012766706	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCG	205	0.5151918775602523	No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	164	0.4121535020482019	No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	162	0.40712723982810184	No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	130	0.3267070443065015	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	122	0.30660199542610134	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	119	0.2990626020959513	No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	112	0.28147068432560124	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	110	0.27644442210550124	No Hit
GAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	107	0.2689050287753512	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	106	0.2663918976653012	No Hit
TTACTCTATCTAGAGATTCGGGAGAAGATAACAAGATAATTAAGCCTAGAA	101	0.25382624211505117	No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	93	0.23372119323465104	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	88	0.221155537684401	No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	85	0.21361614435425097	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	82	0.20607675102410095	No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	81	0.20356361991405092	No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	77	0.19351109547385087	No Hit
ATACTGAGTCTTTCAATTCATGAACATGGAATAGCTTTCCAGTTTTTATGT	69	0.17340604659345077	No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA	68	0.17089291548340077	No Hit
CATATTGATATACTGGCAAAAGGTATAAAGAAAAAACACTTGAAAGATTTG	66	0.16586665326330075	No Hit
GTATTTTGAAAAACGACTAAACCAATGCTTTTTCACTGAAACTGAAATTTA	63	0.15832725993315072	No Hit
ATCTATACGCATCTTAGATCACTGTATATTCAGTTCTGAAAAGCTTCTAAT	63	0.15832725993315072	No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	61	0.15330099771305067	No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	55	0.13822221105275062	No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	53	0.1331959488326506	No Hit
TACCTTCTTCATACGTCATCATTTTGATATAGGCAAAAGAGATTCCTTGGT	51	0.1281696866125506	No Hit
AGAATATACTTCGTCTAGATCCGAAATACGTTTTTATTTCCCCGGTATTCT	51	0.1281696866125506	No Hit
AATATGTAAAGCAGGGAATTCTTTGCATCCATTAAATCGATGTTCATAAAT	50	0.12565655550250057	No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	47	0.11811716217235053	No Hit
TATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	46	0.11560403106230052	No Hit
AATCATTATACAAAAAGAGACACTTGTACTATGTTTATCACAGCACTATTC	45	0.11309089995225051	No Hit
ATTTATATCCATGTGATAACTTCACAGGAGTAAAGATGAACATTTTGGTGA	43	0.10806463773215047	No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	42	0.10555150662210047	No Hit
ATCATAGATACAGTCACATACTGCATAATAATGTTTTGTTCAACAATGGAC	42	0.10555150662210047	No Hit
AGCATATATATAACAACGGAACCGAAGAAGTAATACGTAGAGGGAAGAAAA	42	0.10555150662210047	No Hit
GTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	41	0.10303837551205047	No Hit
ATCCAGTAATTATGGACCATTGTATTTTCATTTGTCTCCATGCACTTAATT	40	0.10052524440200045	No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA	40	0.10052524440200045	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCTAG	20	6.951325E-4	45.0	43
GCCTAGA	20	6.951325E-4	45.0	44
ATCTAGA	20	6.951325E-4	45.0	8
GATAACA	20	6.951325E-4	45.0	27
TCTATCT	20	6.951325E-4	45.0	5
TATCTAG	20	6.951325E-4	45.0	7
ATTAAGC	20	6.951325E-4	45.0	39
ATTCGGG	20	6.951325E-4	45.0	16
AGAGATT	20	6.951325E-4	45.0	12
TAATTAA	20	6.951325E-4	45.0	37
GATTCGG	20	6.951325E-4	45.0	15
TAGAGAT	20	6.951325E-4	45.0	11
TACTCTA	20	6.951325E-4	45.0	2
AAGCCTA	20	6.951325E-4	45.0	42
AGATTCG	20	6.951325E-4	45.0	14
CCTAGAA	20	6.951325E-4	45.0	45
TTCGGGA	20	6.951325E-4	45.0	17
CTATCTA	20	6.951325E-4	45.0	6
AGATAAC	20	6.951325E-4	45.0	26
TCGGGAG	20	6.951325E-4	45.0	18

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content