FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_10.35100000042c73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_10.35100000042c73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26970
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC383614.223210975157583TruSeq Adapter, Index 12 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT2590.9603262884686689No Hit
TTTTTATACTGATCGTGCTTCTATATCAATTAGAAGCTACATTGAGTAGGC1580.585836114200964No Hit
TAATAAAATTGCTTGAAAGAACAAGGTTTGGGGATACTGGTCTTTGGAAAT1300.482017055988135No Hit
ACTTTCAACAATAGATAGATCATCCAGACAGAAAATCAATAGAAAACACTT1280.4746014089729329No Hit
AGTTATTATGGTGTAGATATTTCGGAAAACTATATTGAAGAGGCTACGAAA1240.45977011494252873No Hit
ATGTACTTGCATATCTATCACCACTATTGTAATCTGCTCAGTAAATAGCGT1220.4523544679273267No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1130.4189840563589173No Hit
AAAGAATACAGTCTATTCTATCAACTCTGAACAAACAACTAAAAGAAATAG1090.40415276232851316No Hit
TATTTAATCAGCATATGTTTCACATTGCTTTAGTGTTTTACTCTTTCTTAC1080.40044493882091214No Hit
ATTTAAGATAGTGATTCTGTAGAAAATCAGGTACATTTGATTGACCTTGTC1020.3781979977753059No Hit
ATGTAAGCTGTTTTTTTAATAACCTTGAGGAATTTATCTTTGTTCCTAGTT990.3670745272525028No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA970.3596588802373007No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.3485354097144976No Hit
ATATGGAACTAAAATGTCAAATCAAGGAAGCAAAAGAAGATTTACAAAAGA930.3448275862068966No Hit
TTTTTCTAGTTCTGTGAAGAATGTCATTGATAGTTTGATGGGAATAGCATT930.3448275862068966No Hit
GTGTATGAACATATTTCTAAGTCAGTGTATCCTCAGCAAATTTATTTCTAC900.33370411568409347No Hit
TTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT890.3299962921764924TruSeq Adapter, Index 12 (95% over 22bp)
ACAGTAAAAGGTATCAACTTAATACCAGATGTTGACTTTACAATCACTGAT880.3262884686688914No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA810.3003337041156841No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA800.29662588060808304No Hit
ATAAATAGTTCATCTAAAGTATGGAACAGGAAAATAGAGATGCTGCTGAGT780.28921023359288095No Hit
GAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA780.28921023359288095No Hit
ACTATTAGGTGCATAGTTTTTCCACTTTTGGTTTGAAATGTTAAAAATTGT760.28179458657767886No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.27437893956247683No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCG720.26696329254727474No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.26325546903967373No Hit
TTATTAATGAGCACTTGTATATTTACAGGAAATGGGATAAGCAACACACAA700.25954764553207266No Hit
GTATGTTTATATCCGGAAAAAGTATAGTCTCCTAAACTGTTGGTATGTATG680.25213199851687057No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.25213199851687057No Hit
GGATACTTATGTCAAAAAAAGAAGATGTAATTGTATTAGACGAAGTCATTG670.24842417500926958No Hit
GACCAAATAGATAACATAGGCCAATAAGTAACCTGATACATCCACTTTAAA670.24842417500926958No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA620.22988505747126436No Hit
GTAGTATGACATAAGGTTCTAAATCGTGTCACCATATTGATTTAATTGTAA620.22988505747126436No Hit
ATATCTCACCACATAGTTGTAGTTTAGGTAATAAGTTAATGTGGTGATATA570.21134593993325915No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA570.21134593993325915No Hit
GTTCATGTATTTGATATTCGTTTTAGATATCGCAGATTAATCTTTGTAAGT570.21134593993325915No Hit
ATTCAATACTAGCTCATTTGTTTACAGAATGGAGAAAATTAAAAAGAAGCT560.20763811642565813No Hit
GTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA550.2039302929180571No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA520.19280682239525399No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA510.18909899888765294No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.17797552836484984No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA480.17797552836484984No Hit
GTAATTAACCATTGTGATGTCACAAGTCAACAATATCATCATAATGCTTAT460.17055988134964778No Hit
ATGTAAGAACTGACACACAATAGAAGCTCATTTCAGTTTGTTTTAAATCTG460.17055988134964778No Hit
CAATTGGTTACAAAAATCTGTATTGAAAAATTTCAACACAATTTAGTATGG460.17055988134964778No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA450.16685205784204674No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTTC450.16685205784204674TruSeq Adapter, Index 12 (95% over 24bp)
GAATCAAATAGAATCATCATTGAACAGAATCAAATTGGATCATCATCGAAT450.16685205784204674No Hit
GGATAAATGCTTAACATGATAGAGACTCCATTTATGCTGATGTGATTATTA450.16685205784204674No Hit
CTCTTATCACTATGTGAAATTTCTTGTAAAATTTGGCTGAATGTATGTAGC450.16685205784204674No Hit
AAACTAATCTTGCTGGAATTCAACACAATTTCATCATTTCAAATCAGGACA430.15943641082684462No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA430.15943641082684462No Hit
ATTAATGAGAGTTAGCTTAATATACAGCAAGTTTTACATTCCTGTATATTA420.1557285873192436No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC420.1557285873192436No Hit
GGATAATATGAATGGGAAAAACTGGAATAAAATTCGTTAATGACATCTATT410.15202076381164256No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.15202076381164256No Hit
CTCCTAACATTCTATAGAAATAAGCCTGGAGAGGAAAAGAATGTTCCTAGA380.14089729328883943No Hit
GTATGTCTTCTAAATATACCCTCCTTTATGAATTGGCTTGGATAATTTTAG370.13718946978123842No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA370.13718946978123842No Hit
GTACAAATGTAAATGGGGAGAAATAGTGAAATCTTTTCTGTTTGTCATCAT370.13718946978123842No Hit
ACACATGCTAGTAGAATTTGTAATTTCTTCGTTTTTATTTAGTTTTGAGTG360.13348164627363737No Hit
TTTAAGGTTAGTTTCAGCATCTTTAATTGAATCTTCAGAAAAATTGATAGG360.13348164627363737No Hit
ATTTTAATGTCTGTAGAATCTGTAGTGATATCATCTCACTCATTACTGATA350.12977382276603633No Hit
ATTCTTTTCAATTCGATTCTTTTCGATTCGAGTCTTTTTGATTCGATTCAT350.12977382276603633No Hit
ATCTTGGAGTTAGAGTTACGATAATAGAAGTTGCAGATGACATTTTATTAA350.12977382276603633No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA340.12606599925843529No Hit
AGTATACACTATCAAAATGAAGAAAGGCTTTTAATGTATCATACTTAAGGC340.12606599925843529No Hit
GGATAATTAGCTAGAAATAACAAAAAAAACAGCAAAAAAGAACAAAACAGA330.12235817575083426No Hit
GACTGACTCAATGAATCCTTAATCTCATCAAAATAAGATAGACGGTCTTCT330.12235817575083426No Hit
GTCTTATAGTTGTTTTTCTTTTTCTCTCTTGCTGTTTTCCTTTGTGTTTCA320.11865035224323323No Hit
GAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.11494252873563218No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA310.11494252873563218No Hit
CAATTCAATTAGATGCATTAATCTCTCTAGTGAATGATGGCAAAGCAAAAG300.11123470522803114No Hit
GTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA300.11123470522803114No Hit
GTACGGGATTCTATTTGAATAAGATCATGAGATTCCACCTATACCTCCACC300.11123470522803114No Hit
GGTTATTACAAATGGACCCAATGGATTTTCTTACAGTTATTTCAATCCTGG300.11123470522803114No Hit
ATAGAAAAGGAAGCACTTTTAAATAGATTGAGGATACATAATAATGACCCT280.10381905821282907No Hit
ATATTAAGCTTGTTCAGGCAGAAAACGCTTTAGCTAATTAATCTATGGAAA280.10381905821282907No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA270.10011123470522804No Hit
GATATGTGATTGACCAAAGGAATAAAAGAAGCAAAATATAAACAAGACAGT270.10011123470522804No Hit
GAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.10011123470522804No Hit
AAATAGTGCTTCTAAAAATATAGAGAAGACTTGTTTCTCAACCTTTGTAAA270.10011123470522804No Hit
ATTTAAAGCACAACACCATTTACAAGTAGCACTCCCCAAAATGAAATACCT270.10011123470522804No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAGTT253.790012E-545.00000412
GATTTAC253.790012E-545.00000439
CTTTTGG206.91124E-445.025
TGGTTTG206.91124E-445.029
ACAAAAG206.91124E-445.044
TGCATAG206.91124E-445.010
GTGCATA206.91124E-445.09
GAAATGT206.91124E-445.035
AAATTGT206.91124E-445.045
ATTAGGT206.91124E-445.04
TAAAATG206.91124E-445.010
GTCAAAT206.91124E-445.016
GGTTTGA206.91124E-445.030
TAGTTTT206.91124E-445.014
ACTTTTG206.91124E-445.024
AGGTGCA206.91124E-445.07
TCAAATC206.91124E-445.017
GCAAAAG206.91124E-445.030
GAACTAA206.91124E-445.06
TTAGGTG206.91124E-445.05
Produced by FastQC (version 0.11.2)