FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pcontrolb_1.35100000042b5b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pcontrolb_1.35100000042b5b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84577
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC2818633.325845087908064RNA PCR Primer, Index 27 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGT7100.8394717239911561No Hit
TTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCT6280.7425186516428817RNA PCR Primer, Index 38 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3230.3819005166889343No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3150.37244168036227343No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCG3110.367712262198943No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2270.2683944807690034No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA1980.23410619908485758No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA1810.21400617189070315No Hit
GAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1620.1915414356148835No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA1400.16552963571656598No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1400.16552963571656598No Hit
ATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCTTC1380.16316492663490076RNA PCR Primer, Index 27 (95% over 24bp)
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1370.16198257209406813No Hit
GTATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA1250.14779431760407677No Hit
GTATCAACGCAGAGTACGGGTCGAGGGAGGTCCTAAGAAGAAACGTAAAGT1220.1442472539815789No Hit
GTCCTAAGAAGAAACGTAAAGTTGGTGGCCCAAAAAAAAAAAAAAAAAAAA1180.13951783581824845No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1180.13951783581824845No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1170.13833548127741582No Hit
TATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA1100.13005899949158753No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA1080.1276942904099223No Hit
TATCAACGCAGAGTACGGGTCGAGGGAGGTCCTAAGAAGAAACGTAAAGTT990.11705309954242879No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATAT206.9785566E-445.0230753
TGCCGTC30650.043.23476445
ATGCCGT31650.041.86873244
AGCCCAC32200.041.50293717
TATCTCG32050.041.48658836
ATCTCGT32100.041.4219737
AGACGAA32100.041.4219725
GCTATCT32050.041.4163934
TGCTATC32050.041.4163933
ACGAATT32000.041.41079727
CCCACGA32200.041.36319719
CCACGAG32200.041.36319720
CTCGTAT32150.041.35754839
CTATCTC32150.041.35754835
GAGACGA32100.041.35188324
GACGAAT32050.041.3461926
CGAATTG32000.041.3404928
GAATTGC32000.041.3404929
CACGAGA32300.041.30479421
ACGAGAC32300.041.30479422
Produced by FastQC (version 0.11.2)