FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_96.35100000042b24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_96.35100000042b24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5189
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTC78015.031798034303334No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGT741.4260936596646754No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCG210.40470225476970517No Hit
ATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTCTTC170.32761611100404703TruSeq Adapter, Index 4 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC1000.045.00000417
AATCTCG1000.045.00000436
CAGCAGC1000.045.00000429
GCCCACG1000.045.00000418
CAGCAAT1000.045.00000432
CCACGAG1000.045.00000420
CACGAGA1000.045.00000421
CCCACGA1000.045.00000419
AGCAGCA1000.045.00000430
ACGAGAC1000.045.00000422
CGAGCCC1000.045.00000415
CCGAGCC1000.045.00000414
TCTCCGA1000.045.00000411
CGAGACC1000.045.00000423
AGACCAG1000.045.00000425
TCCGAGC1000.045.00000413
GACCAGC1000.045.00000426
CTCCGAG1000.045.00000412
GAGACCA1000.045.00000424
ACCAGCA1000.045.00000427