Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_1228_pb_95.35100000042b0a.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 8722 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTC | 2178 | 24.97133684934648 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGT | 215 | 2.465030956202706 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCG | 46 | 0.5274019720247649 | No Hit |
ATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTCTTC | 31 | 0.355423068103646 | TruSeq Adapter, Index 4 (95% over 22bp) |
CTTATACACATCTCCGAGCCCACGAGACCATCAGCATCTCGTATGCCGTCT | 17 | 0.19490942444393486 | RNA PCR Primer, Index 18 (95% over 21bp) |
TATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTCTT | 12 | 0.13758312313689522 | RNA PCR Primer, Index 4 (95% over 21bp) |
TTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTCT | 12 | 0.13758312313689522 | RNA PCR Primer, Index 36 (95% over 21bp) |
CTTAATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGT | 9 | 0.10318734235267141 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 260 | 0.0 | 40.673077 | 45 |
ATGCCGT | 285 | 0.0 | 37.105263 | 44 |
ATCTCCG | 290 | 0.0 | 36.46552 | 10 |
TCCGAGC | 290 | 0.0 | 36.46552 | 13 |
AGCCCAC | 295 | 0.0 | 35.847458 | 17 |
GTATGCC | 295 | 0.0 | 35.847458 | 42 |
TATGCCG | 295 | 0.0 | 35.847458 | 43 |
CATCTCC | 295 | 0.0 | 35.847458 | 9 |
GAGCCCA | 295 | 0.0 | 35.847458 | 16 |
CACGAGA | 295 | 0.0 | 35.847458 | 21 |
CCGAGCC | 295 | 0.0 | 35.847458 | 14 |
TCTCCGA | 295 | 0.0 | 35.847458 | 11 |
CTCCGAG | 295 | 0.0 | 35.847458 | 12 |
ACGAGAC | 290 | 0.0 | 35.68966 | 22 |
CGAGACC | 290 | 0.0 | 35.68966 | 23 |
GAGACCA | 290 | 0.0 | 35.68966 | 24 |
ATCTCGT | 300 | 0.0 | 35.250004 | 37 |
CACATCT | 300 | 0.0 | 35.250004 | 7 |
GCCCACG | 300 | 0.0 | 35.250004 | 18 |
CCACGAG | 300 | 0.0 | 35.250004 | 20 |