FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_95.35100000042b0a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_95.35100000042b0a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8722
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTC217824.97133684934648No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGT2152.465030956202706No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCG460.5274019720247649No Hit
ATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTCTTC310.355423068103646TruSeq Adapter, Index 4 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCATCAGCATCTCGTATGCCGTCT170.19490942444393486RNA PCR Primer, Index 18 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTCTT120.13758312313689522RNA PCR Primer, Index 4 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTCT120.13758312313689522RNA PCR Primer, Index 36 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGT90.10318734235267141No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2600.040.67307745
ATGCCGT2850.037.10526344
ATCTCCG2900.036.4655210
TCCGAGC2900.036.4655213
AGCCCAC2950.035.84745817
GTATGCC2950.035.84745842
TATGCCG2950.035.84745843
CATCTCC2950.035.8474589
GAGCCCA2950.035.84745816
CACGAGA2950.035.84745821
CCGAGCC2950.035.84745814
TCTCCGA2950.035.84745811
CTCCGAG2950.035.84745812
ACGAGAC2900.035.6896622
CGAGACC2900.035.6896623
GAGACCA2900.035.6896624
ATCTCGT3000.035.25000437
CACATCT3000.035.2500047
GCCCACG3000.035.25000418
CCACGAG3000.035.25000420