FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_94.35100000042aef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_94.35100000042aef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134738
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTC53623.9795751755258353No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGT13420.9960070655642803No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCG6590.4890973593195683No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1900.14101441315738694No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC1800.13359260193857708No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1760.13062387745105317No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1620.12023334174471939No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1570.11652243613531445No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATAC1540.11429589276967152No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACA1490.1105849871602666No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATA1450.10761626267274266No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6050.042.02306445
TATGGCT250.002097123336.0118834
GATTTAA250.002097123336.0118831
AGATAGT250.002100950835.9985166
TGGCTGA458.627121E-734.9985586
ATGCCGT7700.033.0181244
CTGTGTG352.807943E-432.1415339
CACGAGA8150.031.74715821
ACGAGAC8100.031.66536122
CCACGAG8200.031.55357620
CCCACGA8200.031.27919619
CGAGACC8200.031.27919623
GCCCACG8350.030.71729718
CTCGTAT8250.030.27147939
TATGCCG8400.030.2666143
GCAGCAA8350.030.17839631
ACCAGCA8300.030.08911927
GTATGCC8450.030.08751742
GTGAGTA300.005123049530.0099032
GCCGTCT453.4692297E-529.99876445
Produced by FastQC (version 0.11.2)