FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_93.35100000042ad2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_93.35100000042ad2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190499
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTC51072.6808539677373635No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGT16990.8918681987831957No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCG5810.3049884776298038No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA5320.2792665578296999No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4660.2446207066703762No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4610.24199602097648804No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3610.18950230709872493No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3250.1706045701027302No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA3200.16797988440884204No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2640.13858340463729468No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2510.13175922183318547No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA2380.12493503902907625No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC2110.11076173628208022No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC2060.10813705058819206No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2040.1070871763106368No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA2000.10498742775552627No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA2000.10498742775552627No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA1960.10288767920041575No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC5550.043.77842345
TGGACTA250.002103971835.99558629
GCGGCGT250.002103971835.99558614
GTACGGT250.002103971835.99558614
ATCCTAC352.7963254E-432.1727031
CCTACAC352.7963254E-432.1727033
GGGCAAT352.8136285E-432.1389218
GGTGGAG501.97205E-631.4961411
ATGCCGT7950.030.56229244
TTATAGC602.381621E-730.0278593
GCTGGTG453.4786393E-529.9963258
CAATTAG300.00513968629.99632521
CAGTGGC300.00513968629.99632523
TGTGTCG300.00513968629.99632524
TAGTTCG406.151526E-428.1215539
GAGACCA8800.028.12155324
ACGAGAC8800.028.12155322
CCACGAG8800.028.12155320
AGCAATC8650.028.08904533
CAGCAGC8600.027.99075729
Produced by FastQC (version 0.11.2)