FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_92.35100000042ab8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_92.35100000042ab8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173697
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTC28281.6281225352193764No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGT9490.5463537078936309No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA5340.30743190728682707No Hit
AAAAAGGCTTTGGAAAATATCAACTCCCGTCTTCGTCTTGTTATGAAGAGT4580.2636775534407618No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4070.23431607914932323No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3940.22683178178091734No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3700.2130146174084757No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA3600.207257465586625No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3080.17732027611300138No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCG2780.1600488206474493No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA2710.1560188143721538No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC2700.15544309918996874No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2220.1278087704450854No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2200.12665734008071527No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC2130.12262733380541979No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1930.1111130301617184No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1900.10938588461516319No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA1900.10938588461516319No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA1840.10593159352205277No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA1740.10017444170020208No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3900.042.68520445
TCCCGTC501.0695658E-940.50492525
CCCGTCT552.7175702E-936.8226626
TAGTTCG250.002103913635.994019
ATGCCGT5100.032.6416344
ATATTTA352.8085834E-432.1467671
CCGTCTT651.3873432E-831.15763527
ATATCAA651.391345E-831.14866417
GACCAGC5450.030.55417426
CGAGACC5450.030.55417423
ACGAGAC5450.030.55417422
CCACGAG5450.030.55417420
TATGCCG5450.030.54537843
AGACCAG5400.030.42036425
GTATGCC5500.030.2676942
ATCTCGT5550.030.00364937
CAGCAGC5400.030.00364729
GGATCGA300.00513231830.00364719
GTATATG300.00513231830.0036471
CTCGAGG300.00513231830.00364718
Produced by FastQC (version 0.11.2)