FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_1228_pb_88.35100000042a32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_1228_pb_88.35100000042a32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11696
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC182615.61217510259918No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT2852.4367305061559508No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCG720.615595075239398No Hit
TATCTGACTTATACAACCTCAGCATTAACTAACTCAGAACAGAAACAGAAA530.4531463748290014No Hit
TTGTAAGGTGTATTCCTGTATACTACTTGAGAATACACACTTAGCTGTGCA510.436046511627907No Hit
TAGATCTCATCCATCATTTGAACAAGAATATTGATGCTGTTCTTTTTATCG440.3761969904240766No Hit
AGTTTAGATTGGTAAAAATGCAGAGCAAGAAAAAGAGAACAATAGTTTCAA420.3590971272229822No Hit
CTTGTAAATTTATTGAAGCTCATCTTATGGCTTAACATATGGTCTATTCTG410.350547195622435No Hit
GAATGGAATCATCGAATAAAATTGTTTGAAATCATCATCGAATGGAATCAA400.3419972640218878No Hit
GGAAGATTTAATCTGAAAAGCAGAGTGCAATTCTCAATGCTAATTTTTCAA390.3334473324213406No Hit
CTATATAGATAGTGTGATGAAACAATGGAATATTCCCGGTTTAGCATTAGG380.32489740082079344No Hit
TGATAACTATGTGAGGTGAAGGATATGTTAATTAGCTTGATTGTGGTAATC370.31634746922024626No Hit
TTATTCACAATAGCAAAAAGATGAAAACAACCCAACTGTCCATTAACAGAT340.29069767441860467No Hit
ACTGAATCTTCATTTGAAAAGACTAAAGCATTTGTACCTTTTAATTCGCTT340.29069767441860467No Hit
GTGTATTATATACTTGGAATTTGCTGAGAGGGTAAATCTCAAATATTCTCA340.29069767441860467No Hit
GGATCTATACCTTCCATAAAAGCTTTGATATACAAACCCGTACCACCTACC310.2650478796169631No Hit
GGGTAAGATTAGTAACCTGTTGTGTAGCTACTTTATAATTATGCATCACAG310.2650478796169631No Hit
TATCAGGGTATTGAAATGTTTACTGCTATGCGTAGATTAAACAAACCTGTA290.24794801641586867No Hit
AGTATGAATAAGCAGACAGTACTGCCAATACAGATATTTTAGGCAGTGAAC280.2393980848153215No Hit
ATACCATGTTCCATTTATTTGCTTCCATTCATTTGCAATGTAGGAATTATC280.2393980848153215No Hit
GTTTCTTTTTGATTCGATTCGATTAGAATCTTATGATTCAAGTCTTTTTGA270.23084815321477428No Hit
ATTTTACCTTTGATTGATAAAACTTGATAGTAGTTTCCTGAAACTTTGCTG270.23084815321477428No Hit
TCTAAAAAGGAAAAAGAAAGTTCTTTGAGGATGGAATTGACTGAACTATTT260.22229822161422708No Hit
ATATAAAGGAATTAGCAAGGGAAACTGAGGTAATATTCTTACGATGTGTTG250.2137482900136799No Hit
GTATTGTAGCTATTATTCTTGTTTTATGGGGAATCAGCTACAAGTTAGAAG230.1966484268125855No Hit
GTTAGAAACAATCTACATAATGAAAAGTCACTTTTGGGGTAAGGAAATGAC220.1880984952120383No Hit
ATGTTATTTGGTACTCAAGTTGTAAATAACGTCTACTACTGTTTATTCCAG220.1880984952120383No Hit
GAATCTACGTTGCAGATTAATAATTCAGGGTTTAAGATTTGATAAGCAGAA220.1880984952120383No Hit
ACGTTTGTATACTTTAACAGCATCTGCAATGTCTACAGTATCTGCTTCAAT210.1795485636114911No Hit
TTCCATATACATCCAAATTGTCCTTCATGAAAGAAGACAGAATGAAAATAT210.1795485636114911No Hit
CTCATGAATAAATCAACAAAAATCTTAAACAACAACAACAACAACAAAAAC210.1795485636114911No Hit
GTAATATTTCTCTTGCTTAGATTGCTTTGTCATTTCATCCAGTGACTTCTC200.1709986320109439No Hit
ATACTGTTGCATTCAGGATTAAGTTTCAACATGAATTTTGGAGAGGGCATA200.1709986320109439No Hit
GATACAATTGAAGCAACAGCTAAATGTTTTACAAATGATTTGTCTATGAGT200.1709986320109439No Hit
ATATTGTATTAGCCTACATCTACTCAAAAAATGCACCAACACCTATCACTA200.1709986320109439No Hit
ATTATTGGGAATCCAAATGAAATATTTGGAACAAAGTTTCCATCCATTACA200.1709986320109439No Hit
GGTTCAGTTATATTACCGCTAAAGAAAAACTTAGAGTCATTCAATTTGCAT190.16244870041039672No Hit
ATCATTAATGATGTAATGCCATTCTCTCCTTTTTCATTAATATCGACTCCC190.16244870041039672No Hit
CAATAATGTAACCAATGGAAACTATACGACCATATAAAATCAATTTTCTGT180.1538987688098495No Hit
TAACTAGTCTTAGTTTTGTTCTATCAGACTTGTCATATCATTTCCCTCCAA180.1538987688098495No Hit
CAAATGGAAAAAATTTGTAAGGGATTAGAGTATGGACTTGATGTTTCATTT170.14534883720930233No Hit
CAGTATATCCTCTTCAGAATCTGTTGTTTCAATACCAACCAATACTAATAA170.14534883720930233No Hit
GAGTTAAAGACTTAAACATATCTTTTTTGGAAGGGAACACAACTCAACCTG170.14534883720930233No Hit
CTATAAACAATTAGAAGGCAAACAAAAAATGGATAAGTATTTGCCACATAC160.13679890560875513No Hit
AATGATCATTTTGTCTGTTTTTAGATTGCAAACGACACATATTTATTGTCA160.13679890560875513No Hit
ACTTAGAGGTAAAGGAACATACAAAACATGTTACAAATCTCCATGTTCTAA160.13679890560875513No Hit
ATTATTGCGAGAGGGTAAAACAAAATTAAACGCAACTTTTACTGTTGATAC160.13679890560875513No Hit
TAATAATTCAAGCCAGATTTGTAATTGTTCCTGTTTCTAGAAGTCTAAAAA150.12824897400820792No Hit
TTTCTTAACAGTATTTTCCTGGGTATGAGGATGAGTATCCTTTCAGTTTTA150.12824897400820792No Hit
ACTATGCACAAGAATATGAATTAAACGACGCATTAGAAATTGTTGAAAAAT150.12824897400820792No Hit
AAAGAATATAATCACAAAATCACTGCTGATCGATAGCTATCAAAAACTAGG150.12824897400820792No Hit
AAATCGAAGATATTTTGGAGATAGACTCTTTGAAAGGCTTATTATTGCTAA150.12824897400820792No Hit
TGTCTGTATCCTTATTACTTAGCATAGTACTTGCCTTCATTAAATGCTTGT150.12824897400820792No Hit
GATTTAATTCGATTCTATTCATTTCGATTCGATTCGATTCGAATTTTTCGA150.12824897400820792No Hit
AAATGATACTTAGAGAAAAGTATCATTTCAGCAACTTCTTTACCAGTTAAT150.12824897400820792No Hit
TATTTGAAGGATAACTTTGCTAGATACATTGTTTTGGGTGACAAATTTATT140.11969904240766074No Hit
TAGATAAACCACTCTACAAATATCTAAGCAAACCAATTTCCGAGTATGAAT140.11969904240766074No Hit
CAATTTTGTTAATCCTTTACTAAAACACATGTCAAAGTTCGATTTTTAGTG140.11969904240766074No Hit
GGAAGAATAATTGTTAATGGTGACACAATATCCTAAATCACAGAAGGTATA140.11969904240766074No Hit
GATGTAGGTCATAATATAGATGGCATTACTGAAATCAATAAACAGTTGGAG140.11969904240766074No Hit
GAACAATGTACACCATACTAGCACATCTGATTCACAAAATACTAATGCGTC140.11969904240766074No Hit
GTATTACCGAAGAAATCAATGCGGGTTATTTTGAACAGTACGCTTTACAAA140.11969904240766074No Hit
TTGAAGAGTTAACCAACAATTGGAACTAATCAATGATTTCAGAAAAGTAGC140.11969904240766074No Hit
AACAAAATGGTGAGAAGCTACAGGTTATTACAGGAAAAAAAAATCAGAACA140.11969904240766074No Hit
GTTTTAATTCCGGTATTTTATCGAACTTAACATTGAATGCCTCTTCATCTT130.11114911080711354No Hit
AAGAAAAAAAACAACACACGTTTTCAACTTGGGCAAGTCAATTGTATTTCA130.11114911080711354No Hit
ATATATGCAGTAAGAGTGGAAATCAGATGATAAAATCTACCAGACTACTAG130.11114911080711354No Hit
GTACATAATTGTAAACATCATCAAAGGTTTCAAAATAGACCAAAGATAACC130.11114911080711354No Hit
ATAAAAGTGTGTCACTTGAGGATTGTTGTATTGACAGAACTGTTTTGTATC130.11114911080711354No Hit
GAAGATAGAGAATTATTCCGATCATTGATGAATGAATTAGATGTTCCGGTA130.11114911080711354No Hit
ATTTTGATGATTTGTCTTACACAATGGCAAGATGTGTTAGATTTGTAACAT120.10259917920656635No Hit
AACTTATTGAAGGAAATTTCACAGAAATAGGAAAGCAGGTAAGTTGGCTTT120.10259917920656635No Hit
ACCTAATTCATATAGGTAGATAGCATAAATGGGATGTTGTTTGCCCAATGA120.10259917920656635No Hit
GTATACAACGATGGTTTGTTTTGAAGACATTTATGATTCAAAAAAGCATTG120.10259917920656635No Hit
AAAACTAGACAGAAGCATTCTCAGAAACTTGTTTGTGATGTGTGTATTCAA120.10259917920656635No Hit
ATCCTATAGTGTTTTCTAGGTAAGGCTTATTGTTTTTATCTCGTTTGACTA120.10259917920656635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1900.041.4473745
ATGCCGT2050.038.4146344
ACGTCTT2150.037.6744227
GAGCCCA2150.037.6744216
AGACGTC2150.037.6744225
ACGAGAC2150.037.6744222
GACGTCT2150.037.6744226
CGAGCCC2150.037.6744215
CGAGACG2150.037.6744223
TCCGAGC2150.037.6744213
TTAGTAT2150.037.6744232
CGTCTTA2150.037.6744228
GAGACGT2150.037.6744224
AGTATCT2100.037.50000434
TAGTATC2100.037.50000433
TCTCGTA2100.037.50000438
TATCTCG2100.037.50000436
CTCGTAT2100.037.50000439
AGCCCAC2200.036.8181817
ATCTCCG2200.036.8181810
Produced by FastQC (version 0.11.2)